MiR-148b Caused Liver Injury in Rats with Traumatic Hemorrhagic Shock by Inhibiting SIRT6 Expression.

Background: The purpose of this study was to investigate the role of miR- 148b in liver injury in rats with traumatic hemorrhagic shock (THS) and to elucidate its potential mechanism.

Methods: The levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in the serum of rats were detected by enzyme-linked immune sorbent assay (ELISA), and the injury of rat liver was analyzed by hematoxylin-eosin (H&E) staining. Apoptosis of rat hepatocytes and normal rat liver cell line (BRL3A) was identified by terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL) assay and flow cytometry, respectively.

Groundwater nitrate pollution risk assessment of the groundwater source field based on the integrated numerical simulations in the unsaturated zone and saturated aquifer.

Groundwater pollution risk assessment in the groundwater source field (GSF) is crucial to ensure groundwater quality safety. A systematic method of assessing groundwater pollution in the GSF was established by combining the numerical models of groundwater flow and solute transport in the vadose zone and aquifer. It is featured by revealing the paramount fate of contaminant from the surface to receptor "well (wells)" via the pathway of vadose zone and aquifers.