Preliminary study on the preparation of lyophilized acellular nerve scaffold complexes from rabbit sciatic nerves with human umbilical cord mesenchymal stem cells.

Background: The gold standard of care for patients with severe peripheral nerve injury is autologous nerve grafting; however, autologous nerve grafts are usually limited for patients because of the limited number of autologous nerve sources and the loss of neurosensory sensation in the donor area, whereas allogeneic or xenografts are even more limited by immune rejection. Tissue-engineered peripheral nerve scaffolds, with the morphology and structure of natural nerves and complex biological signals, hold the most promise as ideal peripheral nerve "replacements".

Aim: To prepare allogenic peripheral nerve scaffolds using a low-toxicity decellularization method, and use human umbilical cord mesenchymal stem cells (hUC-MSCs) as seed cells to cultivate scaffold-cell complexes for the repair of injured peripheral nerves.

Lyophilized bovine acellular tendon linear fiber material for the reconstruction of attachment structure of paraspinous muscles: an animal in vivo study.

Low back pain is common after lumbar spine surgery and the injury from extensive detachment of paraspinal muscles during the surgery may play a vital role. Previously, we prepared a bovine acellular tendon fiber (ATF) material through lyophilization and proved that it could retain its original fibrillar structure and mechanical properties. The objective of this study is to evaluate the effectiveness of this new fiber material used for attachment structure reconstruction of paraspinal muscle.

Aponeurosis discission, a low-detergent method for tissue-engineered acellular ligament scaffolds.

Detergent treatment is the most commonly used method for the decellularization of ligaments and tendon grafts. However, it is well recognized that detergent treatment can also adversely affect the extracellular matrix. This study found that discission into the aponeurosis layer of the patellar tendon (PT) before decellularization is conducive to extracting cells from the PT using a low quantity of detergent in a short time period.

Consensus statement on diagnosis and treatment of cervical ossification of posterior longitudinal ligament from Asia Pacific Spine Society (APSS) 2020.

Purpose: The study aimed to develop an evidence-based expert consensus statement on diagnosis and treatment of cervical ossification posterior longitudinal ligament (OPLL).

Method: Delphi method was used to perform such survey, and the panel members from Asia Pacific Spine Society (APSS) 2020 were invited to answer the open-ended questions in rounds 1 and 2. Then the results were summarized and developed into a Likert-style questionnaire for voting in round 3, and the level of agreement was defined as 80%.

A novel revision surgery for treatment of cervical ossification of the posterior longitudinal ligament after initial posterior surgery: preliminary clinical investigation of anterior controllable antidisplacement and fusion.

Background: Cervical ossification of the posterior longitudinal ligament (OPLL) is a progressive disease. Posterior decompression surgery is reported to be an effective and comparatively safe procedure with few complications for treatment of patients with myelopathy caused by OPLL. However, some patients require revision surgery because of late neurological deterioration due to OPLL progression or kyphotic changes in cervical alignment.

Evaluation of Measuring Methods of Spinal Canal Occupation Rate in Thoracic Ossification of Ligamentum Flavum.

Objective: The degree of hypertrophy of thoracic ossification of ligamentum flavum (TOLF) is related to the severity of the myelopathy. There is no uniform measuring method to calculate the spinal canal occupation ratio (COR) of TOLF simply and effectively. The study was to determine an appropriate measuring method to calculate the COR of TOLF.

Imaging grading system for the diagnosis of dural ossification based on 102 segments of TOLF CT bone-window data.

Thoracic ossification of the ligamentum flavum (TOLF) complicated with dural ossification (DO) is a severe clinical disease. The diagnosis of DO preoperatively remains challenging. The current study retrospectively analyzed imaging features of 102 segments with TOLF from 39 patients and proposed a grading system for evaluating DO risk.

Rapid prototyping technology and its application in bone tissue engineering.

Bone defects arising from a variety of reasons cannot be treated effectively without bone tissue reconstruction. Autografts and allografts have been used in clinical application for some time, but they have disadvantages. With the inherent drawback in the precision and reproducibility of conventional scaffold fabrication techniques, the results of bone surgery may not be ideal.

Fibroblast growth factor 2 induces mesenchymal stem cells to differentiate into tenocytes through the MAPK pathway.

Mesenchymal stem cells (MSCs) differentiate into numerous different cell types and thus have therapeutic potential for tissue engineering, anti‑inflammatory and immunomodulatory purposes. FGF2 may affect the biological behavior of MSCs. MSCs were transduced with either adenovirus‑null vector/green fluorescent protein (GFP) or a vector encoding for the overexpression of FGF2/GFP.

A universal pedicle screw and V-rod system for lumbar isthmic spondylolysis: a retrospective analysis of 21 cases.

Objective: To investigate the surgical outcome of a universal pedicle screw-V rod system and isthmic bone grafting for isthmic spondylolysis.

Methods: Twenty-four patients with isthmic spondylolysis at L5 and grade 0-I spondylolisthesis (Meyerding classification) received isthmic bone graft and stabilization using the universal pedicle screw-V rod system. Back pain was evaluated using the visual analog scale (VAS) and time to bone healing, improvement in spondylolisthesis and intervertebral space height at L5/S1 and L4/L5 were assessed.

Identification of risk factors for respiratory complications in upper cervical spinal injured patients with neurological impairment.

Objective: Clinical features of respiratory complications in patients with upper cervical spine injuries (CSI) are unique due to the complex regional anatomy in the region. The objective of this study was to identify the risk factors for respiratory complications in the patients with upper CSI and cervical spinal cord injuries (C-SCI).

Methods: Ninety-two patients (out of 1593 spine injured patients) who met the inclusion criteria of upper CSI were admitted to our hospital from 1992 to 2010.

[Clinical observation and evaluation of reasons for pseudarthrosis after anterior cervical fusion].

Objective: To explore the causes of pseudarthrosis and evaluate the clinical neurological function and neck subaxial symptoms after anterior cervical fusion.

Methods: A total of 412 patients were followed up with an average of 5.4 years.

[Short-term clinical results of interspinous dynamic fixation of Coflex for the prevention of adjacent segment degeneration after lumbar fusion].

Objective: To observe the short-term clinical results of the adjacent segment degeneration after the implantation of Coflex system at the interspinous space of adjacent segment to lumbar fusion.

Methods: Fifty patients with grade III disc (Thompson MRI classification) of adjacent segment to lumbar fusion were included and divided alternately into two groups according to the order of hospitalization from January to November 2009. Coflex system was implanted at the interspinous space of adjacent segment to lumbar fusion in 25 patients as Coflex group, the other 25 patients did not have any surgical treatment were as control group.

En bloc resection of lamina and ossified ligamentum flavum in the treatment of thoracic ossification of the ligamentum flavum.

Objective: We performed a retrospective patient chart review to determine the feasibility and safety of en bloc resection of lamina and ossified ligamentum flavum in the treatment of thoracic ossification of ligamentum flavum (OLF).

Methods: From January 2000 to June 2006, 36 patients with thoracic OLF underwent en bloc resection of lamina and ossified ligamentum flavum by a burr-grinding technique. The range of resection included one lamina superior and one lamina inferior to the diseased segments.

The genetic association of vitamin D receptor polymorphisms and cervical spondylotic myelopathy in Chinese subjects.

Background: The association of vitamin D receptor (VDR) gene polymorphisms to the lumbar degenerative disc disease has been previously studied; however, the role of VDR gene polymorphisms in cervical spondylosis remains unknown.

Methods: One hundred fifty four patients with cervical spondylotic myelopathy (CSM) and 156 controls were enrolled. The clinical characteristics were collected and the severity of cervical spondylotic myelopathy was evaluated by magnetic resonance imaging (MRI).

The clinical features and surgical treatment of degenerative lumbar scoliosis: a review of 112 patients.

Objective: To investigate the clinical features, radiological characteristics and surgical results of degenerative lumbar scoliosis (DLS).

Methods: One hundred and twelve cases of DLS treated surgically from June 2001 to February 2006 were retrospectively reviewed for clinical features, characteristics of nerve root compression and imaging presentations. According to the preoperative clinical manifestations and imaging findings, different surgical modalities were performed, including simple nerve decompression and decompression with short or long posterior fusion (less or more than three segments, respectively).

Clinical significance of cervical vertebral flexion and extension spatial alignment changes.

Study Design: Radiographic review.

Objective: To study changes in alignment and curvature of the cervical vertebral column in the sagittal plane, and to provide references for the orthopedic treatment of cervical kyphosis.

Summary And Background Data: There are few reports analyzing changes of the vertebral body itself or changes in their relationships between separate vertebrae.

[Sagittal diameters measurements on MR of the cervical spinal cord in normal subjects].

Objective: To offer normal reference of diameter of the cervical spinal cord and available diameter of cervical spinal canal and to screen scientific radiographic criteria to define and quantify cervical spinal cord disease.

Methods: The magnetic resonance images of 120 normal people had been measured. The data of diameters of cervical spinal cord, CSF, M, the ratio of diameters of cord and CSF, and the ratio of diameters of cord and M had been collected and statistical analysis was made.

[Clinical characteristics and diagnosis of rheumatoid arthritis of upper cervical spine: analysis of 71 cases].

Objective: To study the clinical characteristics and diagnosis of rheumatoid arthritis (RA) in the upper cervical spine.

Methods: The clinical data of 71 patients with RA in the upper cervical spine, 18 males and 53 females, aged 46.2 (23-76), with a mean duration of RA of 18.

[Anterior radical decompression for severe ossification of the posterior longitudinal ligament in the cervical spine].

Objective: To report the outcome of anterior radical decompression for the treatment of severe ossification of the posterior longitudinal ligament (OPLL) with an average occupying ratio exceeding 50% in the cervical spine.

Methods: From July 2002 to February 2006, 26 patients with cervical OPLL occupying ratio of the spinal cord exceeding 50% underwent anterior decompression and fusion. There were 18 males and 8 females.

[Time of early death after cervical spinal cord injury].

Objective: To investigate the distribution of time of early death in the patients with cervical spinal cord injury (CSCI).

Methods: The clinical data of 63 CECI patients, including demographics, mechanism of injury, cervical spinal cord injury level and severity, associated injury, radiographs, management, and the causes of death, the time from injury to hospitalization and the time from injury to death were retrospectively analyzed so as to detect the time and cause of early death.

Results: The 63 CDCI patients died in early stage.

[Surgical strategy for severe cervical spine dislocations].

Objective: To evaluate the rate of open reduction and surgical strategy of severe cervical dislocation.

Methods: From March 2001 to March 2006, the data of 92 cases of cervical dislocation over 1/2 were retrospectively studied. Garden Well traction with 1 - 3 kg weight were performed before operation.

[Diagnosis and treatment for the injury of cervical disc and longitudinal ligament].

Objectives: To study the diagnosis and treatment for the injury of cervical disc and longitudinal ligament.

Methods: From 2001 to 2005, the clinical data of sixty-three patients with cervical disc and longitudinal ligament injury were studied. Early treatment was done based on spinal cord injuries and spinal stabilities by X rays and MRI.

[Surgical treatment and its related factors of cervical spondylotic myelopathy].

Objective: To observe the results and its related factors of surgical treatment of cervical spondylotic myelopathy (CSM).

Methods: Totally 365 CSM patients were reviewed. All patients were treated with anterior cervical decompression and fusion with autogenous iliac bone or titanium mesh cages with local bone graft.

[Key points about atlanto-axial internal-fixation and fusion using Gallie's technique].

Objective: To study the clinical problems about posterior atlanto-axial internal-fixation and fusion for atlanto-axial instability or dislocation.

Methods: Surgical treatments of 138 cases with atlanto-axial instability or dislocation were reviewed. There were 62 cases of odentoid malformation, 54 cases of odentoid fracture or rupture of transverse ligament, 22 cases of subluxation and rotation.