Three-Dimensional Bioprinting of Decellularized Extracellular Matrix-Based Bioinks for Tissue Engineering.

Three-dimensional (3D) bioprinting is one of the most promising additive manufacturing technologies for fabricating various biomimetic architectures of tissues and organs. In this context, the bioink, a critical element for biofabrication, is a mixture of biomaterials and living cells used in 3D printing to create cell-laden structures. Recently, decellularized extracellular matrix (dECM)-based bioinks derived from natural tissues have garnered enormous attention from researchers due to their unique and complex biochemical properties.

Inhibitory effect of silymarin on CCl-induced liver fibrosis by reducing Ly6C monocytes infiltration.

It has been well established that silymarin has hepatoprotective and anti-fibrotic effects. But the mechanisms are poorly understood. In recent years, the role of Ly6C monocytes in liver fibrosis has been well demonstrated.

Protective effects of curcumin against liver fibrosis through modulating DNA methylation.

Recent research has demonstrated that advanced liver fibrosis in patients could be reversed, but no approved agents are available for the treatment and prevention of liver fibrosis in humans. Curcumin (CUR) is the principal curcuminoid of turmeric. Inhibitory effects of CUR and its underlying mechanisms in liver fibrogenesis have been explored.

Association of serum gamma-glutamyl transferase with treatment outcome in chronic hepatitis B patients.

Aim: To investigate the association of serum gamma-glutamyl transferase (GGT) levels with chronic hepatitis B infection and hepatitis B e antigen (HBeAg) seroconversion.

Methods: A retrospective study was performed on clinical data collected from patients who had been positive for hepatitis B surface antigen for > 6 mo and who were antiviral-treatment naïve (n = 215) attending the Hepatitis Clinic at Nanjing Drum Tower Hospital between August 2010 and December 2013. Healthy individuals without liver disease (n = 83) were included as controls.

[The correlation between expression level of femB and resistance phenotype of methicillin-resistant Staphylococcus aureus (MRSA)].

Objective: To investigate the correlation between the phenotype and expression level of femB of Staphylococcus aureus (MRSA), to discuss the mechanism of different phenotypes in methicillin-resistant Staphylococcus aureus.

Methods: The minimal inhibitory concentrations (MICs) of oxacillin against 71 clinical isolates of Staphylococcus aureus were determined by agar dilution method according to NCCLS. The production of beta-lactamase was identified by Cefinase paper strip method.

[Study on the correlation between resistance phenotype and expression level of femA of Staphylococcus aureus].

Objective: To detect the expression level of femA of Staphylococcus aureus strains with different phenotype.

Methods: 15 strains of the non-beta-lactamase-producing clinical isolates with different phenotype by agar dilution and by nitrocephin paper strip method were chosen as the object of test, in addition to 4 donative strains (BB270, BB308, BB586, COL). Total RNA were extracted and analysed by agarose gel electrophoresis.

[A study on the mechanism of drug resistance in Staphylococcus aureus].

Objective: To inquire into the mechanism of drug resistance in Staphylococcus aureus.

Methods: A total of 198 strains of Staphylococcus aureus were isolated from the samples sent to the Clinical Laboratory of Microbiology,West China Hospital. The resistance of Staphylococcus aureus to Methicillin was assayed with agar dilution.