Publications by authors named "Xiomara Fernandez-Garibay"
Muscular dystrophies are a heterogeneous group of highly debilitating diseases that result in muscle atrophy and weakness. The lack of suitable cellular and animal models that reproduce specific aspects of their pathophysiology is one of the reasons why there are no curative treatments for these disorders. This highlights a considerable gap between current laboratory models and clinical practice.
View Article and Find Full Text PDFBioengineered human skeletal muscle tissues have emerged in the last years as newsystems for disease modeling. These bioartificial muscles are classically fabricated by encapsulating human myogenic precursor cells in a hydrogel scaffold that resembles the extracellular matrix. However, most of these hydrogels are derived from xenogenic sources, and the culture media is supplemented with animal serum, which could interfere in drug testing assays.
View Article and Find Full Text PDFMyotonic dystrophy type 1 (DM1) is the most common hereditary myopathy in the adult population. The disease is characterized by progressive skeletal muscle degeneration that produces severe disability. At present, there is still no effective treatment for DM1 patients, but the breakthroughs in understanding the molecular pathogenic mechanisms in DM1 have allowed the testing of new therapeutic strategies.
View Article and Find Full Text PDFMuscular dystrophies are a group of highly disabling disorders that share degenerative muscle weakness and wasting as common symptoms. To date, there is not an effective cure for these diseases. In the last years, bioengineered tissues have emerged as powerful tools for preclinical studies.
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- Understanding protein secretion from specific tissues is essential for improving disease detection and treatment methods, but measuring these dynamics in live organisms is challenging.
- The study introduces a platform for near real-time measurement of cytokines from a 3D tissue model, using advanced techniques like a Transwell insert and magnetic microbead detection.
- Demonstrating its effectiveness, the platform can track inflammation-related proteins IL-6 and TNF-α in response to electrical and chemical stimuli in muscle tissues, providing a new and cost-effective way to study metabolic disorders and drug effects.
Article Synopsis
- Despite advancements in organs-on-a-chip technology, integrating real-time sensing for biomarkers in 3D tissues has been limited.
- The study introduces a custom platform for stimulating muscle cells in a fluid environment, connected to a sensitive electrochemical sensing system for monitoring interleukin 6 and tumor necrosis factor alpha.
- This innovative setup allows for precise, cost-effective measurements of biomarker release from 3D muscle microtissues during different stimulations, supporting metabolic research.
New biocompatible materials have enabled the direct 3D printing of complex functional living tissues, such as skeletal and cardiac muscle. Gelatinmethacryloyl (GelMA) is a photopolymerizable hydrogel composed of natural gelatin functionalized with methacrylic anhydride. However, it is difficult to obtain a single hydrogel that meets all the desirable properties for tissue engineering.
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