Structural characterization of a novel glucan from Achatina fulica and its antioxidant activity.

A novel glucan designated AFPS-IB was purified from Achatina fulica (China white jade snail) by anion-exchange and gel-permeation chromatography. Chemical composition analysis indicated AFPS-IB was composed of glucose, fucose, rhamnose, mannose, and galactose in a molar ratio of 189:2:1:1:2 and with an average molecular weight of 128 kDa. Its structural characteristics were investigated by Fourier transform infrared spectroscopy (FTIR), high performance liquid chromatography (HPLC), gas chromatography mass spectrometry (GC-MS), methylation analysis, nuclear magnetic resonance (NMR) spectroscopy ((1)H,( 13)C, H-H COSY, HSQC, TOCSY, and NOESY), and atomic force microscopy (AFM).

Depolymerization of fucosylated chondroitin sulfate from sea cucumber, Pearsonothuria graeffei, via 60Co irradiation.

A method for depolymerization of a novel fucosylated chondroitin sulfate from Pearsonothuria graeffei (fCS-Pg) using (60)Co irradiation in water solution was developed in the current study. Fragments with varying molecular weights were obtained by (60)Co irradiation at different dosages and sample concentrations. The chemical compositions and structures of these fragments were further investigated using high-performance liquid chromatography (HPLC), infrared spectroscopy (IR) and nuclear magnetic resonance spectroscopy (NMR).