Bifunctional MOF-Encapsulated Cobalt-Doped Carbon Dots Nanozyme-Powered Chemiluminescence/Fluorescence Dual-Mode Detection of Aflatoxin B1.

A novel bifunctional MOF-encapsulated cobalt-doped carbon dots nanozyme (Co-CD/PMOF) with excellent peroxidase-mimic catalytic activity and fluorescence property was synthesized and employed to fabricate a chemiluminescence/fluorescence (CL/FL) dual-mode immunosensor for AFB1 detection. Co-CD/PMOF could catalyze the luminol/HO system to generate robust and long-lasting CL signals due to the slow diffusion effect and continuous generation of •OH, O, and O species. Differing from traditional flash-type CL emissions, this glow-type CL emission is helpful to fabricate a sensitive and accurate CL sensing platform.