Inhibition of CRISPR-Cas12a trans-cleavage by lead (II)-induced G-quadruplex and its analytical application.

In this work, the inhibition of clustered regularly interspaced short palindromic repeats (CRISPR) - CRISPR associated protein (Cas) trans-cleavage by Pb-induced G-quadruplex has been firstly explored to detect Pb in tea beverage and milk. In absence of Pb, the Na-induced G-quadruplex can be cleaved by CRISPR-Cas12a. In contrast, Pb can competitively combine with G-quadruplex, resulting in its conformational changes and resistance to trans-cleavage of CRISPR-Cas12a.

Bioactive substances and potentiality of marine microalgae.

Microalgae is one of the most important components in the aquatic ecosystem, and they are increasingly used in food and medicine production for human consumption due to their rapid growth cycle and survival ability in the harsh environment. Now, the exploration of microalgae has been gradually deepening, mainly focused on the field of nutrition, medicine, and cosmetics. A great deal of studies has shown that microalgae have a variety of functions in regulating the body health and preventing disease, such as nitrogen fixation, antitumor, antivirus, antioxidation, anti-inflammatory, and antithrombotic.

Fabrication of Pickering emulsion based on particles combining pectin and zein: Effects of pectin methylation.

Although the stabilization of Pickering emulsions based on the interaction of pectin and zein is known to be effective, the effects of the degree of methylation (DM) of pectin on the stability of Pickering emulsions remains unclear. In the present study, pectin/zein particle dispersions (PZDs) were fabricated by anti-solvent precipitation based on different DM values of pectin (13.70 %-71.

Application of gas sensors for modelling the dynamic growth of Pseudomonas in pork stored at different temperatures.

Pseudomonas have a faster growth rate over other bacteria in chilled meat under aerobic conditions. A non-destructive method for modelling the dynamic growth of Pseudomonas in pork stored at different temperatures using gas sensors was presented in our work. Based on selected gas sensor data, the first-order kinetic equations (Gompertz and Logistic Functions) combined with the secondary model (Square-root Function) effectively simulated Pseudomonas growth in pork at different temperatures with R and RMSE values of 0.

Dynamic changes of proton populations in potato flours during fermentation and their relationship with digestibility and functional properties.

The effects of fermentation on the dynamic state of proton populations, digestibility, and functional properties of potato flours prepared from Atlantic and Kexin No. 1 were investigated. Depending on the changes in moisture content and digestibility of potato flours during fermentation, three proton populations were distinguished.

Effects of Fermentation on Compositions, Color, and Functional Properties of Gelatinized Potato Flours.

This study investigated effects of fermentation on compositions, color, and functional properties of gelatinized potato flours from Atlantic and Kexin No.1 cultivars. Atlantic flour (AF) and Kexin No.

Investigating lignin from Canna edulis ker residues induced activation of α-amylase: Kinetics, interaction, and molecular docking.

The lignin isolated from C. edulis ker residues showed a significant activating effect on α-amylase. Further studies revealed that the isolated lignin formed a 1:1 complex with α-amylase through hydrogen bonding and quenched fluorescence of α-amylase with a static quenching procedure.

Evaluation of lipid oxidation of Chinese-style sausage during processing and storage based on electronic nose.

A portable electronic nose was used for extracting flavour fingerprint map of Chinese-style sausage during processing and storage, in parallel with detection of acid value (AV) and peroxide value (POV) for evaluating lipid oxidation. Sausage samples during processing and storage were divided into three and five quality phases, respectively. After comparison of sensors response to lipid oxidation, optimal sensor array was determined.

Hyperspectral imaging detection of decayed honey peaches based on their chlorophyll content.

Honey peach is a very common but highly perishable market fruit. When pathogens infect fruit, chlorophyll as one of the important components related to fruit quality, decreased significantly. Here, the feasibility of hyperspectral imaging to determine the chlorophyll content thus distinguishing diseased peaches was investigated.

Predicting the growth situation of Pseudomonas aeruginosa on agar plates and meat stuffs using gas sensors.

A rapid method of predicting the growing situation of Pseudomonas aeruginosa is presented. Gas sensors were used to acquire volatile compounds generated by P. aeruginosa on agar plates and meat stuffs.

Growth Simulation and Discrimination of Botrytis cinerea, Rhizopus stolonifer and Colletotrichum acutatum Using Hyperspectral Reflectance Imaging.

This research aimed to develop a rapid and nondestructive method to model the growth and discrimination of spoilage fungi, like Botrytis cinerea, Rhizopus stolonifer and Colletotrichum acutatum, based on hyperspectral imaging system (HIS). A hyperspectral imaging system was used to measure the spectral response of fungi inoculated on potato dextrose agar plates and stored at 28°C and 85% RH. The fungi were analyzed every 12 h over two days during growth, and optimal simulation models were built based on HIS parameters.