Nursing Cost Analysis for Acute Exacerbation of Chronic Obstructive Pulmonary Disease in the Intensive Care Unit.

Objective: To determine nursing costs for intensive care unit (ICU) patients with acute exacerbation of chronic obstructive pulmonary disease (AECOPD), assess the correlation with diagnosis-related group (DRG) payments and identify cost determinants.

Design: Prospective, descriptive and quantitative study.

Methods: From January to December 2022, we selected ICU patients with AECOPD and used time-driven activity-based costing method to calculate the overall nursing costs.

Single-cell and spatial RNA sequencing reveal the spatiotemporal trajectories of fruit senescence.

The senescence of fruit is a complex physiological process, with various cell types within the pericarp, making it highly challenging to elucidate their individual roles in fruit senescence. In this study, a single-cell expression atlas of the pericarp of pitaya (Hylocereus undatus) is constructed, revealing exocarp and mesocarp cells undergoing the most significant changes during the fruit senescence process. Pseudotime analysis establishes cellular differentiation and gene expression trajectories during senescence.

CsMYC2 is involved in the regulation of phenylpropanoid biosynthesis induced by trypsin in cucumber (Cucumis sativus) during storage.

Trypsin has a new activity of scavenging superoxide anion and generating hydrogen peroxide. Trypsin can significantly improve the storage quality of C. sativus.

Structural Characterization, Rheological Properties, Antioxidant and Anti-Inflammatory Activities of Polysaccharides from Zingiber officinale.

The structural characteristics, rheological properties, antioxidant and anti-inflammatory activities of Zingiber officinale polysaccharides (ZOP) and ZOP-1 were studied. The total soluble sugar contents of ZOP and ZOP-1 were 78.6 ± 0.

Transcriptome analyses and virus-induced gene silencing identify HuWRKY40 acting as a hub transcription factor in the preservation of Hylocereus undatus by trypsin.

Trypsin can significantly improve the storage quality of Hylocereus undatus (H. undatus). To verify the hub WRKY gene of H.

EuRBG10 involved in indole alkaloids biosynthesis in Eucommia ulmoides induced by drought and salt stresses.

Alkaloids are natural products with many important medicinal activities. To explore the mechanism of abiotic stress promoting alkaloid biosynthesis in Eucommia ulmoides, transcriptomic analysis and metabonomic analysis were used, virus-induced gene silencing (VIGS) lines of target gene were constructed. The results showed that drought and salt stress caused wilting and blackening of leaves, decreased chlorophyll level, and significantly induced MDA and relative conductivity.

Omics analyses indicate sdhC/D act as hubs of early response of E. coli to antibiotics.

In recent years, the phenomenon of microbial resistance has become increasingly serious. The generation of reactive oxygen species (ROS) during the bactericidal process of antibiotics has attracted great interest, but little research has been done on the generation of ROS in the early stage of antibiotic action. We confirmed the rapid production of ROS by flow cytometry and transmission electron microscopy (TEM).

Lipid metabolism regulated by superoxide scavenger trypsin in Hylocereus undatus through multi-omics analyses.

To analyze the mechanism of the effect of trypsin on the preservation of Hylocereus undatus, the transcriptomic and widely targeted metabolomic profiles of H. undatus after trypsin treatment were evaluated. Among 477 genes related to lipid metabolism, 32 genes had significant expression differences.

Comprehensive evaluation of Loblolly fruit by high performance liquid chromatography four wavelength fusion fingerprint combined with gas chromatography fingerprinting and antioxidant activity analysis.

Loblolly fruit (LBF) is mainly used as raw material for beverage, but there are few researches on its quality evaluation or control. The aim of this study was to develop comprehensive evaluation methods for the quality control of Loblolly fruit. firstly, double wavelength coefficient ratio spectrum was used to identify the purity of chromatographic fingerprint peak.

Impact of the National Centralized Drug Procurement Policy (4 + 7 policy) on the drug expenditures of patients treated in outpatient and emergency departments in a large tertiary level-A hospital in China: A single centre, interrupted time series.

What Is Known And Objective: Like many countries in the world, China is also facing growing drug expenditures year by year. In particular, the rising cost of prescription drugs has been one of the critical factors leading to the serious burden on health insurance programs. The high cost of prescription drugs not only threatens the health budget but also limits the nation's investment in other public sectors.

LsrB, the hub of ABC transporters involved in the membrane damage mechanisms of heavy ion irradiation in .

Background: Ionizing radiation, especially heavy ion (HI) beams, has been widely used in biology and medicine. However, the mechanism of membrane damage by such radiation remains primarily uncharacterized.

Purpose: Transcriptomic profiles of () treated with HI illustrated the response mechanisms of the membrane, mainly ABC transporters, related genes regulated by antibiotics treatment through enrichment analyses of GO and KEGG.

Omics analyses indicate the routes of lignin related metabolites regulated by trypsin during storage of pitaya (Hylocereus undatus).

The storage quality of Hylocereus undatus was significantly improved by trypsin, a novel preservative. The transcriptomic results revealed that antioxidant signal pathways were induced, while lignin catabolic process was impeded by trypsin. In addition, the results of protein-protein interaction (PPI) network networks suggested that flavone 3'-O-methyltransferase 1 (OMT1), ferulic acid 5-hydroxylase 1 (CYP84A1), cellulose synthase isomer (CEV1), and 4-coumarate-CoA ligase 3 (4CL3) act as hubs of peroxidases, lignin related proteins, and proteins involved in the phenylpropanoid metabolism (PLPs) induced by trypsin.

PFOA regulate adenosine receptors and downstream concentration-response cAMP-PKA pathway revealed by integrated omics and molecular dynamics analyses.

As an important pollutant, perfluorooctane acid (PFOA) has been widely concerned and reported by thousands of times, while less is known about the concentration-response pathway of PFOA. The aim of the present work was to reveal the concentration-response mechanism of PFOA in human cells. Omics results showed that calcium-related pathways play key roles in PFOA injury mechanisms.

Catechin gallate acts as a key metabolite induced by trypsin in Hylocereus undatus during storage indicated by omics.

Trypsin is a novel superoxide scavenger. The storage quality of H. undatus was significantly improved by trypsin.

Entirely control the quality consistency of Rong'e Yishen oral liquid by both quantified profiling and quantitative analysis of multi-components by single marker method.

Quantified profiling and quantitative analysis of multi-components by single marker (QAMS) method were combined to control the quality consistency of Rong'e Yishen oral liquid (REYS) in this paper. Firstly, High Performance Liquid Chromatography (HPLC) with diode array detection (DAD) was applied to collect fingerprints and establish a content determination method. Then, two methods of QAMS and the external standard method (ESM) were used for comparative study to investigate the feasibility of the former to accurately control the quality of individual marker.

Hub genes and sub-networks of stoma-related genes in Hylocereus undatus through trypsin treatment during storage revealed by transcriptomic analysis.

To further investigate the preservation mechanisms of trypsin, the synergistic mechanisms of trypsin and stoma-related genes were evaluated in Hylocereus undatus. Trypsin significantly induced the stoma closure and improved the storage quality of H. undatus.

Transcriptomic analysis reveals hub genes and subnetworks related to ROS metabolism in Hylocereus undatus through novel superoxide scavenger trypsin treatment during storage.

Background: It was demonstrated in our previous research that trypsin scavenges superoxide anions. In this study, the mechanisms of storage quality improvement by trypsin were evaluated in H. undatus.

Transcriptomic Analysis Reveals Cu/Zn SODs Acting as Hub Genes of SODs in Induced by Trypsin during Storage.

It has been revealed by us that superoxide scavenging is a new activity of trypsin. In this study, the synergistic mechanisms of trypsin and superoxide dismutases (SODs) were evaluated in (pitaya). Trypsin significantly improved the storage quality of , including weight loss impediment and decrease of cellular injury.

Transcriptomic analysis reveals key genes related to antioxidant mechanisms of Hylocereus undatus quality improvement by trypsin during storage.

It has been revealed in our previous studies that trypsin scavenges superoxide anions. In the current study, the mechanisms of storage quality improvement by trypsin were evaluated in H. undatus.

Ammopiptanthus mongolicus stress-responsive NAC gene enhances the tolerance of transgenic Arabidopsis thaliana to drought and cold stresses.

Drought and cold are the primary factors limiting plant growth worldwide. The Ammopiptanthus mongolicus NAC11 (AmNAC11) gene encodes a stress-responsive transcription factor. Expression of the AmNAC11 gene was induced by drought, cold and high salinity.

Exploring the membrane toxicity of decabromodiphenyl ethane (DBDPE): Based on cell membranes and lipid membranes model.

Decabromodiphenyl ethane (DBDPE) is widely used in industry as an alternative to the decabromodiphenyl ether (BDEs). The large-scale use of DBDPE could lead to rapid growth of the human accumulation level of DBDPE. However, the biophysics of accumulation of DBDPE in cell membranes, as one of determinants of DBDPE metabolism is not clear.

Trypsin Slows the Aging of Mice due to Its Novel Superoxide Scavenging Activity.

Trypsin is an endogenous enzyme that is generally used as a proteinase. Intriguingly, we found that trypsin had superoxide scavenging activity. In the current study, our results showed that trypsin scavenges superoxide in either intracorporal or extracorporal systems.

Comparative transcriptome profiling of a desert evergreen shrub, Ammopiptanthus mongolicus, in response to drought and cold stresses.

Background: The molecular mechanisms involved in plant tolerance to either drought or cold have been extensively studied in many plant species. However, few studies have focused on their comparisons especially using non-model plants with strong tolerance to both stresses. Ammopiptanthus mongolicus (Maxim.