Hsa_circ_0125356 promotes gemcitabine resistance by modulating WNT canonical and non-canonical pathways via miR-582-5p/FGF9 axis in non-small cell lung cancer.

Background: Non-small cell lung cancer (NSCLC) is the leading cause of cancer morbidity and mortality worldwide. The prognosis of patients has been significantly improved by chemotherapy, but acquired drug resistance remains a major obstacle to NSCLC treatment. Circular RNAs (circRNAs), which act as miRNA or protein sponges, are critically associated with the development and chemotherapy resistance of NSCLC.

Genome analysis of a newly isolated Lysinibacillus fusiformis-YC01 for biodegrading inosine and guanosine.

Hyperuricemia (HUA) caused by high serum uric acid (UA) level can lead to a range of metabolic diseases, such as gout, cardiovascular disease and diabetes. The reduction of crucial UA precursors of both inosine and guanosine is a potential method to control HUA. Here a promising bacterial strain for biodegrading both inosine and guanosine were successfully isolated from Baijiu cellar mud and identified as Lysinibacillus fusiformis-YC01 by ANI analysis.

Infrared Small Target Detection Algorithm Based on Improved Dense Nested U-Net Network.

Infrared weak and small target detection technology has attracted much attention in recent years and is crucial in the application fields of early warning, monitoring, medical diagnostics, and anti-UAV detection.With the advancement of deep learning, CNN-based methods have achieved promising results in general-purpose target detection due to their powerful modeling capabilities; however, CNN-based methods cannot be directly applied to infrared small targets due to the disappearance of deep targets caused by multiple downsampling operations. Aiming at these problems, we proposed an improved dense nesting and attention infrared small target detection method based on U-Net called IDNA-UNet.

Photothermal-Responsive Soluble Microneedle Patches for Meibomian Gland Dysfunction Therapy.

Meibomian gland dysfunction (MGD) is a leading cause of evaporative dry eye disease, presenting a challenge for targeted treatment. Traditional topical ocular drug delivery methods often fail to effectively reach the meibomian glands (MGs). To address this, the study has developed a soluble microneedles (MN) patch comprising poly(vinyl alcohol), cyclodextrin modified polyacrylic acid, and new indocyanine green.

Preclinical and First-In-Human Imaging of Novel [F]F-FAPI-FUSCC-07 Tracer: Comparative Prospective Study with [F]F-FAPI-42 and [F]F-FAPI-74.

This study aimed to develop and evaluate a novel fibroblast activation protein (FAP)-specific tracer, fluorine-18-labeled fibroblast activation protein inhibitor-FUSCC-07 ([F]F-FAPI-FUSCC-07), for use in both preclinical and clinical settings. Preclinical evaluations were conducted to assess the stability and partition coefficient of [F]F-FAPI-FUSCC-07. Experiments involving human glioma U87MG cells demonstrated its cellular uptake and inhibitory properties.

Cytotoxicity of Vanadium(IV) and Vanadium(V) on Caco-2 Cells: The Important Influence of Vanadium Speciation.

Exposure to vanadium (V) occurs through the ingestion of contaminated water, polluted soil, V-containing foods and medications, and the toxicity and absorption during the small intestine phase after oral ingestion play crucial roles in the ultimate health hazards posed by V. In this study, the human colon adenocarcinoma (Caco-2) cells were selected as an intestinal absorption model to investigate the uptake and cytotoxicity of vanadyl sulfate (VOSO) and sodium orthovanadate (NaVO). Our results confirmed the cytotoxic effects of V(IV) and V(V) and revealed a greater toxicity of V(IV) than V(V) towards Caco-2 cells.

Theoretical Study on the High Polymer Molecular Weight of Heteroatom-Substituted Constrained Geometry Catalyst.

This theoretical study investigates the high molecular weight (Mw) production in copolymerization of ethylene and 1-octene using heteroatom-substituted constrained geometry catalysts (CGCs). The research explores the correlation between chain termination reactions and polymer molecular weight, revealing that the Gibbs free energy barrier of the chain termination reactions is positively linked to the molecular weight. Quantitative structure-property relationship models were constructed, indicating that molecular descriptors such as atom charge, orbital energy, and buried volume significantly influence the polymer molecular weight.

Just-in-Time Simulation Training to Augment Overnight ICU Resident Education.

Patients who decompensate overnight experience worse outcomes than those who do so during the day. Just-in-time (JIT) simulation could improve on-call resident preparedness but has been minimally evaluated in critical care medicine (CCM) to date. To determine whether JIT training can improve residents' performance in simulation and if those skills would transfer to better clinical management in adult CCM.

Metabolomic Analysis of Carotenoids Biosynthesis by sp. USTB-05.

Carotenoids belonging to the class of tetraterpenoids have extensive applications in medicine, food, nutrition, cosmetics, and feed. Among them, lutein and zeaxanthin can prevent macular degeneration in the elderly, which is very important for protecting vision. Here, we introduce the first metabolomic analysis of sp.

Engineered Mesenchymal Stromal Cell Exosomes-Loaded Microneedles Improve Corneal Healing after Chemical Injury.

Corneal alkali burns represent a prevalent ophthalmic emergency with the potential to induce blindness. The main contributing mechanisms include excessive inflammation and delayed wound healing. Existing clinical therapies have limitations, promoting the exploration of alternative methods that offer improved efficacy and reduced side effects.

Anion Substitution to Suppress the Voltage Hysteresis of NaMnTi(PO) as a Cathode Material for Sodium-Ion Batteries.

The Mn-based polyanion compound NaMnTi(PO) (NMTP) with a Na superionic conductor (NASICON) structure has attracted incremental attention as a potential cathode material for sodium-ion batteries. However, the occupation of Mn on Na vacancies usually leads to severe voltage hysteresis, which in turn results in significant capacity loss, slow Na diffusion kinetics, and poor cycling stability. Herein, anion-substituted compounds NaMnTi(PO)(SiO) ( = 0.

Enzymatic mechanism of MlrB for catalyzing linearized microcystins by sp. USTB-05.

Microcystins (MCs) are the most widespread cyanobacterial toxins in eutrophic water body. As high toxic intermediate metabolites, linearized MCs are further catalyzed by linearized microcystinase (MlrB) of sp. USTB-05.

Head-to-head comparison of [Ga]Ga-DOTA-FAPI-04 and [F]FDG PET/CT for the evaluation of tonsil cancer and lymph node metastases: a single-centre retrospective study.

Background: This study aimed to compare the diagnostic value of [ Ga]Ga-DOTA-FAPI-04 and [F]FDG PET/CT imaging for primary lesions and metastatic lymph nodes in patients with tonsil cancer.

Method: Twenty-one tonsil cancer patients who underwent [ Ga]Ga-DOTA-FAPI-04 and [F]FDG PET/CT scans within two weeks in our centre were retrospectively enrolled. The maximum standardized uptake value (SUVmax) and tumor-to-background ratio (TBR) of the two tracers were compared by using the Mann‒Whitney U test.

The combination of high-frequency QRS and ST-segment alterations during exercise stress tests enhanced the diagnostic efficacy for coronary artery disease.

Background: High-frequency QRS (HF-QRS) manifests as a novel adjunct electrocardiographic marker with potential utility in coronary artery disease (CAD) detection.

Hypothesis: We hypothesize that HF-QRS analysis may be superior to conventional ST-segment analysis in detecting CAD, and the combination of these two analyses in the exercise stress test may enhance the diagnostic efficacy for CAD.

Methods: The study incorporated a sample of 157 patients (mean age 62   9 years) referred for nonemergent angiography.

Biomimetic microfluidic chips for toxicity assessment of environmental pollutants.

Various types of pollutants widely present in environmental media, including synthetic and natural chemicals, physical pollutants such as radioactive substances, ultraviolet rays, and noise, as well as biological organisms, pose a huge threat to public health. Therefore, it is crucial to accurately and effectively explore the human physiological responses and toxicity mechanisms of pollutants to prevent diseases caused by pollutants. The emerging toxicological testing method biomimetic microfluidic chips (BMCs) exhibit great potential in environmental pollutant toxicity assessment due to their superior biomimetic properties.

Diagnosis and treatment of recurrent syncope in a middle-aged women.

A 50-year-old female patient, presented with repeated syncope for more than 2 years. Prior assessments were conducted at different hospitals, but no definite abnormalities were found. The patient's fear and anxiety about possible future attacks were escalating.

Imaging of Tumor Stroma Using Ga-FAPI PET/CT to Improve Diagnostic Accuracy of Primary Tumors in Head and Neck Cancer of Unknown Primary: A Comparative Imaging Trial.

The low detection rate of primary tumors by current diagnostic techniques remains a major concern for patients with head and neck cancer of unknown primary (HNCUP). Therefore, in this study, we aimed to investigate the potential role of Ga-labeled fibroblast activation protein inhibitor (Ga-FAPI) PET/CT compared with F-FDG PET/CT for the detection of primary tumors of HNCUP. In this prospective comparative imaging trial conducted at Fudan University Shanghai Cancer Center, 91 patients with negative or equivocal findings of a primary tumor by comprehensive clinical examination and conventional imaging were enrolled from June 2020 to September 2022.

Preclinical Evaluation and a Pilot Clinical Positron Emission Tomography Imaging Study of [Ga]Ga-FAPI-FUSCC-II.

Fibroblast activation protein (FAP), a type II integral membrane serine protease, is a promising target for tumor diagnosis and therapy. OncoFAP has been recently discovered for PET imaging procedures for various solid malignancies. In this study, we presented the development of manual radiolabeling procedures for the preparation of OncoFAP-based radiopharmaceuticals for cancer imaging.

Chlorogenic acid regulates the expression of NPC1L1 and HMGCR through PXR and SREBP2 signaling pathways and their interactions with HSP90 to maintain cholesterol homeostasis.

Background: Hypercholesterolemia is widely implicated in the etiology of coronary heart disease, stroke, and dementia. Evidence suggests that chlorogenic acid (CA) reduces the risk of cardiovascular disease.

Purpose: The current study aims to explore the underlying molecular mechanism of CA in lowering cholesterol based on pregnane X receptor (PXR) and sterol regulatory element-binding protein 2 (SREBP2) regulatory pathways and their interactions with heat shock protein 90 (HSP90).

Photocurable and Temperature-Sensitive Bioadhesive Hydrogels for Sutureless Sealing of Full-Thickness Corneal Wounds.

Penetrating corneal wounds can cause severe vision impairment and require prompt intervention to restore globe integrity and minimize the risk of infection. Tissue adhesives have emerged as a promising alternative to suturing for mitigating postoperative complications. However, conventional water-soluble adhesives suffer formidable challenges in sealing penetrating corneal wounds due to dilution or loss in a moist environment.

calcium-binding protein Cs16 causes acute hepatic injury possibly by reprogramming the metabolic pathway of bone marrow-derived monocytes.

Introduction: infection results in various complications in the liver and biliary systems and is a neglected tropical disease in Eastern Asia. In this study, we report that calcium-binding protein Cs16 activates host immune cells and induces immunopathology in liver.

Methods: Immunohistochemistry was used to detect the localization of Cs16 in adult worms.

Biodegradation of Inosine and Guanosine by YD01.

Both inosine and guanosine are precursors of uric acid that may cause the diseases of hyperuricemia and gout in humans. Here, a promising bacterial strain for efficiently biodegrading both inosine and guanosine was successfully isolated from a healthy human intestine and identified as YD01 with 16S rRNA analysis. An initial amount of 49.