New insights into the interactions between the antibiotic enrofloxacin and fish protein by spectroscopic, thermodynamic, and theoretical simulation approaches.

In this study, myofibrillar proteins (MPs) from crucian carp were utilized as a model to investigate the binding mechanism between fish proteins and antibiotic residues. Fluorescence quenching confirmed the static quenching (K = 1.89 × 10 M s, K = 1.

Bloch-Zener oscillation with engineered Floquet energy bands in synthetic temporal lattices.

Here we experimentally demonstrate the dynamics of Bloch-Zener oscillations (BZOs) in a synthetic temporal lattice formed by the optical pulses in coupled fiber loops. By periodically modulating the phases imposed to the optical pulses in linear driven lattices, a two-band Floquet system with tunable bandgaps is realized, and the related BZOs that occurred in this system are displayed. On this basis, by manipulating the phase difference and coupling angle of the synthetic lattice, the widths of 0-gap and -gap are tuned feasibly so that a wide variety of the interplays between Bloch oscillations and Landau-Zener tunneling (LZT) are exhibited.

Unveiling the Correlation between Protein, Protein Corona, and Target Signal Loss in SERS Detection.

The application of surface-enhanced Raman scattering (SERS) technology is hindered by the protein corona in a protein-rich complex matrix, which is a hot and important issue that needs consideration. However, the impact of the protein corona on SERS detection has not been fully studied. Herein, we selected three proteins, α-lactalbumin (α-La), β-lactoglobulin (β-Lg), and bovine serum albumin (BSA), as models for forming a protein corona, and melamine was employed as the target in SERS detection.

Anxiety caused by chronic exposure to methylisothiazolinone in zebrafish: Behavioral analysis, brain histology and gene responses.

Methylisothiazolinones (MIT) are a class of preservatives and biocides extensively utilized in everyday products, industrial processes, and medical and healthcare applications. However, reports have indicated that MIT may cause skin irritation and neurotoxicity. Given its pervasive use, the neurotoxic potential of MIT has garnered increasing attention.

Decrease of Prolylcarboxypeptidase Dose of Aqueous Humor is Involved in the Pathogenesis of Primary Open-Angle Glaucoma via Finetuning of the Local Ocular Renin-Angiotensin System.

Objective: In this study, we investigated the cause of the AngII dose elevation in aqueous humor of primary open-angle glaucoma (POAG) patients.

Methods: Enzyme-linked immunosorbent assay (ELISA), western blotting were used to detect concentration of Angiotensin Converting Enzyme 2 (ACE2) and Prolylcarboxypeptidase (PRCP). AngII and AngII + Recombinant PRCP were injected into anterior chamber of mouse eye.

Proteomics and Microbiota Conjoint Analysis in the Nasal Mucus: Revelation of Differences in Immunological Function in and .

All eight pangolin species, especially captive , are critically endangered and susceptible to various pathogenic microorganisms, causing mass mortality. They are involved in the complement system, iron transport system, and inflammatory factors. exhibited a higher abundance of opportunistic pathogens, , which potentially evaded complement-mediated immune response by reducing C5 levels and counteracting detrimental effects through transferrin neutralization.

Temporal Goos-Hänchen Shift in Synthetic Discrete-Time Heterolattices.

Experimental demonstration of tunable temporal Goos-Hänchen shift (GHS) in synthetic discrete-time heterolattices with scalar and vector gauge potentials is reported. By using Heaviside-function modulation in two fiber loops, we create a sharp gauge-potential interface and observe temporal GHS for total internal reflection (TIR), which manifests as a time delay rather than a spatial shift. The TIR occurs as the incident mode falls into the band gap of transmitted region with band shifting by scalar and vector potential.

Observation of discrete-light temporal refraction by moving potentials with broken Galilean invariance.

Refraction is a basic beam bending effect at two media's interface. While traditional studies focus on stationary boundaries, moving boundaries or potentials could enable new laws of refractions. Meanwhile, media's discretization plays a pivotal role in refraction owing to Galilean invariance breaking principle in discrete-wave mechanics, making refraction highly moving-speed dependent.

Subtype prediction of intrahepatic cholangiocarcinoma using dynamic contrast-enhanced ultrasound.

Objective: The study aimed to investigate the predictive value of dynamic contrast-enhanced ultrasound (DCE-US) in differentiating small-duct (SD) and large-duct (LD) types of intrahepatic cholangiocarcinoma (ICC).

Methods: This study retrospectively enrolled 110 patients with pathologically confirmed ICC lesions who were subject to preoperative contrast-enhanced ultrasound (CEUS) examinations between January 2022 and February 2023. Patients were further classified according to the subtype: SD-type and LD-type, and an optimal predictive model was established and validated using the above pilot cohort.

Absolute distance meter without dead zone based on free-running dual femtosecond lasers.

Absolute distance measurements based on femtosecond lasers have been extensively studied for precision metrology and advanced manufacturing, with the advantages of traceability, high speed, and nanometer precision. However, in previous studies, the dual femtosecond laser ranging system showed limitations such as system complexity, lower integration, dead zone problems in single optical path detection, and high requirements for laser coherence. It is challenging to achieve a high degree of integration and large-scale continuous measurements using femtosecond lasers, ineluctably limiting practical applications in engineering fields.

A multi-parameter intrahepatic cholangiocarcinoma scoring system based on modified contrast-enhanced ultrasound LI-RADS M criteria for differentiating intrahepatic cholangiocarcinoma from hepatocellular carcinoma.

Purpose: To develop a multi-parameter intrahepatic cholangiocarcinoma (ICC) scoring system and compare its diagnostic performance with contrast-enhanced ultrasound (CEUS) liver imaging reporting and data system M (LR-M) criteria for differentiating ICC from hepatocellular carcinoma (HCC).

Methods: This retrospective study enrolled 62 high-risk patients with ICCs and 62 high-risk patients with matched HCCs between January 2022 and December 2022 from two institutions. The CEUS LR-M criteria was modified by adjusting the early wash-out onset (within 45 s) and the marked wash-out (within 3 min).

HDL-C and creatinine levels at 1 month are associated with patient 12-month survival rate after kidney transplantation.

Background: Many factors affect the survival rate after kidney transplantation, including laboratory tests, medicine therapy and pharmacogenomics. Tacrolimus, mycophenolate mofetil and methylprednisolone were used as an immunosuppressive regimen after kidney transplantation. The primary goal of this study was to investigate the factors affecting the tacrolimus concentrations and mycophenolate mofetil area under the curve of mycophenolic acid AUC-MPA.

Oncological prognosis and morbidity of hepatectomy in elderly patients with hepatocellular carcinoma: a propensity score matching and multicentre study.

Purpose: With increasing life expectancy, the number of elderly patients (≥ 65 years) with hepatocellular carcinoma (HCC) has steadily increased. Hepatectomy remains the first-line treatment for HCC patients. However, the prognosis of hepatectomy for elderly patients with HCC remains unclear.

Quantitative analysis of pre-treatment dynamic contrast-enhanced ultrasound for assessing the response of colorectal liver metastases to chemotherapy plus targeted therapy: a dual-institutional study.

Objectives: To investigate the clinical value of pre-treatment quantitative contrast-enhanced ultrasound (CEUS) in assessing the response of colorectal liver metastases (CRLM) to chemotherapy plus targeted therapy.

Methods: This study retrospectively enrolled 50 CRLM patients from the Zhongshan Hospital, Fudan University as the training cohort and 14 patients from Shanghai Tenth People's Hospital as the testing cohort. Patients underwent the CEUS examination before receiving chemotherapy (CAPOX, FOLFOX, FOLFIRI, or FOLFOXIRI) plus targeted therapy (Bevacizumab or Cetuximab).

Study of Herbs Cortex Moutan, Poria cocos, and Alisma orientale and Periodontitis.

Introduction: The Chinese traditional herbs Cortex Moutan, Poria cocos, and Alisma orientale are considered to have potential to ameliorate periodontitis, although the possible underlying mechanisms remain mostly unknown. Due to the complex formulation of Chinese herbs, it is important to understand the mechanisms of pharmacologic effects of traditional herbs for better application in modern medical treatment.

Methods: Network pharmacology was applied to explore the mechanism of Cortex Moutan, Poria cocos, and Alisma orientale.

Alteromonas Aquimaris sp. nov., Isolated from Surface Seawater.

A Gram-negative, aerobic, motile by flagellum, and rod-shaped bacterium, designated ASW11-7, was isolated from coastal surface seawater sample collected from the Yellow Sea, PR China. Strain ASW11-7 grew optimally at 37℃, 4.0% (w/v) NaCl and pH 7.

Alteromonas salexigens sp. nov., isolated from coastal seawater.

A Gram-negative, aerobic, short rod-shaped bacterium, designated ASW11-19, was isolated from a coastal seawater sample of the Yellow Sea, PR China. Strain ASW11-19 grew optimally at 37 °C, 3.0-5.

A Bionic Compass Based on Multiradicals.

The underlying chemical and physical mechanism of avian navigation is an important issue of broad interest. One of the most famous candidates is the radical-pair mechanism, which shows that the magnetoreception is achieved by detecting the amount of chemical-reaction product from the singlet state. In the hypothesis, the surrounding nuclear spins play an important role as inducing the coherent transition between the singlet and triplet states.

Fluorescent intracellular imaging of reactive oxygen species and pH levels moderated by a hydrogenase mimic in living cells.

The catalytic generation of H in living cells provides a method for antioxidant therapy. In this study, an [FeFe]-hydrogenase mimic [Ru + FeS@F127(80)] was synthesized by self-assembling polymeric pluronic F-127, catalytic [FeS] sites, and photosensitizer Ru(bpy) . Under blue light irradiation, hydrated protons were photochemically reduced to H, which increased the local pH in living cells (HeLa cells).

Deaminative Arylation and Alkenyaltion of Aliphatic Tertiary Amines with Aryl and Alkenylboronic Acids via Nitrogen Ylides.

Transition-metal-catalyzed Suzuki-Miyaura coupling has significantly advanced C-C bond formation and has been well recognized in organic synthesis, pharmaceuticals, materials science and other fields. In this rapid development, cross coupling without transition metal catalyst is a big challenge in this field, and using widely existing tertiary amines as electrophiles to directly couple with boronic acids has great hurdles yet significant application prospects. Herein, we report an efficient and general deaminative arylation and alkenylation of tertiary amines (propargyl amines, allyl amines and 1H-indol-3-yl methane amines) with ary and alkenylboronic acids enabled by difluorocarbene under transition-metal-free conditions.

Small extracellular vesicles derived from mesenchymal stem cell facilitate functional recovery in spinal cord injury by activating neural stem cells the ERK1/2 pathway.

Spinal cord injury (SCI) causes severe neurological dysfunction leading to a devastating disease of the central nervous system that is associated with high rates of disability and mortality. Small extracellular vesicles (sEVs) derived from human umbilical cord mesenchymal stem cells (hucMSC-sEVs) have been explored as a promising strategy for treating SCI. In this study, we investigated the therapeutic effects of the intralesional administration of hucMSC-sEVs after SCI and determined the potential mechanisms of successful repair by hucMSC-sEVs.

Lanthanide Complexes for Tumor Diagnosis and Therapy by Targeting Sialic Acid.

Sialic acid (SA) is overexpressed on cell membranes of tumor cells, and increased serum SA concentration has been observed in tumor-bearing patients. Herein, a series of lanthanide-containing bimetallic complexes (TDA-M-Lns) for targeting SA were prepared via coordination among luminescent lanthanide ions (Ln = Tb, Eu, Dy, or Sm), metal ion quenchers (M = Cu or Co), and the organic ligand 2,2'-thiodiacetic acid (TDA). SA can competitively coordinate with Ln, resulting in the "signal-on" of the Ln.