Contrasting Dynamics of Intracellular and Extracellular Antibiotic Resistance Genes in Response to Nutrient Variations in Aquatic Environments.

The propagation of antibiotic resistance in environments, particularly aquatic environments that serve as primary pathways for antibiotic resistance genes (ARGs), poses significant health risks. The impact of nutrients, as key determinants of bacterial growth and metabolism, on the propagation of ARGs, particularly extracellular ARGs (eARGs), remains poorly understood. In this study, we collected microorganisms from the Yangtze River and established a series of microcosms to investigate how variations in nutrient levels and delivery frequency affect the relative abundance of intracellular ARGs (iARGs) and eARGs in bacterial communities.

Adaptation of the anammox process for high ammonium photovoltaic wastewater treatment.

Tunnel Oxide Passivating Contacts (TOPcon) battery in the photovoltaic industry generates high ammonium wastewater during the production process, the adaptability of using the anaerobic ammonia oxidation (Anammox) process for photovoltaic wastewater (PVW) treatment is a research hotspot. Based on the analysis of photovoltaic wastewater quality, the effectiveness of nitrogen removal, sludge characteristics and microbial communities were examined. The results showed that when the influent NH-N concentration of PVW was lower than 150 mg·L, the nitrogen removal efficiency (NRE) was almost 100 %.

Functional effects of drugs and toxins interacting with Na1.4.

Na1.4 is a voltage-gated sodium channel subtype that is predominantly expressed in skeletal muscle cells. It is essential for producing action potentials and stimulating muscle contraction, and mutations in Na1.

The Evolution, Expression Patterns, and Domestication Selection Analysis of the Annexin Gene Family in the Barley Pan-Genome.

Plant annexins constitute a conserved protein family that plays crucial roles in regulating plant growth and development, as well as in responses to both biotic and abiotic stresses. In this study, a total of 144 annexin genes were identified in the barley pan-genome, comprising 12 reference genomes, including cultivated barley, landraces, and wild barley. Their chromosomal locations, physical-chemical characteristics, gene structures, conserved domains, and subcellular localizations were systematically analyzed to reveal the certain differences between wild and cultivated populations.

Review of the phenotypes and genotypes of Bardet-Biedl syndrome from China.

To analyze the phenotypes, genotypes, and the relationship of phenotypes and genotypes for Chinese patients with Bardet-Biedl syndrome (BBS). The Chinese Wanfang and Weipu data, and PubMed were searched up to December 2022. Patients with detailed clinical feature data were involved in the analysis.

The Inhibitory Effect of Magnolol on the Human TWIK1 Channel Is Related to G229 and T225 Sites.

TWIK1 (K2P1.1/KCNK1) belongs to the potassium channels of the two-pore domain. Its current is very small and difficult to measure.

Differential dose-response patterns of intracellular and extracellular antibiotic resistance genes under sub-lethal antibiotic exposure.

Although antibiotics are one of the most significant factors contributing to the propagation of antibiotic resistance genes (ARGs), studies on the dose-response relationship at sub-lethal concentrations of antibiotics remain scarce, despite their importance for assessing the risks of antibiotics in the environment. In this study, we constructed a series of microcosms to investigate the propagation of intracellular (iARGs) and extracellular (eARGs) ARGs in both water and biofilms when exposed to antibiotics at various concentrations (1-100 μg/L) and frequencies. Results showed that eARGs were more abundant than iARGs in water, while iARGs were the dominant ARGs form in biofilms.

Spatial Cognition of the Visually Impaired: A Case Study in a Familiar Environment.

Objectives: This paper aims to explore the factors influencing the spatial cognition of the visually impaired in familiar environments.

Background: Massage hospitals are some of the few places that can provide work for the visually impaired in China. Studying the spatial cognition of the visually impaired in a massage hospital could be instructive for the design of working environments for the visually impaired and other workplaces in the future.

Surface Curvature Dominated Guest-Induced Nonequilibrium Deformations of Single Covalent Organic Framework-300 Particles.

Understanding the guest-induced dynamic deformation process of covalent organic frameworks (COFs) is vitally important to further increase their stimulus-response performances. Here we report on the dark-field microscopic (DFM) imaging approach to in situ monitor the guest-induced deformation evolution of individual COF-300 crystals in real time. We observe not only transient and nonequilibrium intermediate deformation states but also local surface curvature-driven diverse adsorption behaviours of single COF-300 particles for dichloromethane (DCM), undergoing one, two, and multiple expansion-contraction deformations as well as contraction-to-expansion transition.

A feasibility investigation of a pilot-scale bioelectrochemical coupled anaerobic digestion system with centric electrode module for real membrane manufacturing wastewater treatment.

The large-scale application of bioelectrochemical coupled anaerobic digestion (BES-AD) is limited by the matching of electrode configuration and the applicability of real wastewater. In this study, a pilot-scale BES-AD system with an effective system volume of 5 m and a 1 m volume of a carbon fiber brush electrode module was constructed and tested for treatment of the membrane manufacturing wastewater. The results showed that the BOD/COD of the wastewater was increased from 0.

Promising therapeutic aspects in human genetic imprinting disorders.

Genomic imprinting is an epigenetic phenomenon of monoallelic gene expression pattern depending on parental origin. In humans, congenital imprinting disruptions resulting from genetic or epigenetic mechanisms can cause a group of diseases known as genetic imprinting disorders (IDs). Genetic IDs involve several distinct syndromes sharing homologies in terms of genetic etiologies and phenotypic features.

The microbial synergy and response mechanisms of hydrolysis-acidification combined microbial electrolysis cell system with stainless-steel cathode for textile-dyeing wastewater treatment.

Microbial electrolysis cell (MEC) has been existing problems such as poor applicability to real wastewater and lack of cost-effective electrode materials in the practical application of refractory wastewater. A hydrolysis-acidification combined MEC system (HAR-MECs) with four inexpensive stainless-steel and conventional carbon cloth cathodes for the treatment of real textile-dyeing wastewater, which was fully evaluated the technical feasibility in terms of parameter optimization, spectral analysis, succession and cooperative/competition effect of microbial. Results showed that the optimum performance was achieved with a 12 h hydraulic retention time (HRT) and an applied voltage of 0.

Dysregulated adipose tissue expansion and impaired adipogenesis in Prader-Willi syndrome children before obesity-onset.

Objective: Prader-Willi syndrome (PWS) is a rare genetic imprinting disorder resulting from the expression loss of genes on the paternally inherited chromosome 15q11-13. Early-onset life-thriving obesity and hyperphagia represent the clinical hallmarks of PWS. The noncoding RNA gene SNORD116 within the minimal PWS genetic lesion plays a critical role in the pathogenesis of the syndrome.

Combination of variational mode decomposition and coherent factor for ultrasound computer tomography.

Background: Ultrasound computed tomography (USCT) is a promising technique for improving the detection of breast cancer. Image quality of USCT has a major impact on the breast cancer diagnosis.

Objective: This paper investigates the combination of variational mode decomposition (VMD) and coherent factor method for USCT image quality enhancement.

CircZNF652 promotes the goblet cell metaplasia by targeting the miR-452-5p/JAK2 signaling pathway in allergic airway epithelia.

Background: Circular RNAs (circRNAs) play potentially important roles in various human diseases; however, their roles in the goblet cell metaplasia of asthma remain unknown.

Objective: We sought to investigate the potential role and underlying mechanism of circZNF652 in the regulation of allergic airway epithelial remodeling.

Methods: The differential expression profiles of circRNAs were analyzed by transcriptome microarray, and the effects and mechanisms underlying circZNF652-mediated goblet cell metaplasia were investigated by quantitative real-time PCR, RNA fluorescence in situ hybridization, Western blot, RNA pull-down, and RNA immunoprecipitation analyses.

Bioelectrochemical systems with a cathode of stainless-steel electrode for treatment of refractory wastewater: Influence of electrode material on system performance and microbial community.

The large-scale application of the bioelectrochemical system (BES) is limited by the cost-effective electrode materials. In this study, five kinds of stainless-steel materials were used as the cathode of the BES coupled with anaerobic digestion (BES-AD) for the treatment of diluted N, N-dimethylacetamide (DMAC) wastewater. Compared with a carbon-cloth cathode, BES-AD with a stainless-steel cathode had more engineering due to its low cost, although the operating efficiencies were slightly inferior.

Visual colorimetric detection of triacetone triperoxide based on a Fe(II)-promoted thermal decomposition process.

Triacetone triperoxide (TATP) explosives, a popular choice for terrorists, have been used in many violent terrorist attacks all over the world. However, simple, rapid, and on-site detection methods of TATP are still lacking. Herein, we present a visual colorimetric method for on-site and rapid detection of TATP based on a Fe(II)-promoted thermal decomposition process of TATP.

Combining electrochemical nitrate reduction and anammox for treatment of nitrate-rich wastewater: A short review.

Treatment of nitrate-rich wastewater is important but challenging for the conventional biological denitrification process. Here, we propose combining the electrochemical reduction and anaerobic ammonium oxidation (anammox) processes together for treatment of nitrate-rich wastewater. This article reviews the mechanism and current research status of electrochemical reduction of nitrate to ammonium as well as the mechanism and applicability of the anammox process.

β-aminobutyric acid induces priming defence against Botrytis cinerea in grapefruit by reducing intercellular redox status that modifies posttranslation of VvNPR1 and its interaction with VvTGA1.

Either NPR1 or TGA1 serve as master redox-sensitive transcriptional regulators for the transcription of PR genes in plants. The redox modification of the two co-activators involved in BABA-induced priming resistance against Botrytis cinerea in grapes was examined in this study. The results showed that 10 mmol L BABA could effectively trigger a priming defense in grapes as manifested by augmented expression levels of PR genes upon inoculation with B.

[Correlation of phosphorylated S6 protein expression in blood and brain tissue in mice and rats with kainic acid-induced seizure].

Objective: To determine the correlation of phosphorylated ribosomal S6 protein (P-S6) content in blood and brain tissue in mice and rats with seizure.

Methods: Seizure models were induced by intraperitoric injection of kainic acid (KA) in C57BL/mice and SD rats. Flow cytometry was used to detect the content of P-S6 in blood; Western blot was used to detect the expression of P-S6 in brain tissues.