Benchmarking cell type annotation methods for 10x Xenium spatial transcriptomics data.

Background: Imaging-based spatial transcriptomics technologies allow us to explore spatial gene expression profiles at the cellular level. Cell type annotation of imaging-based spatial data is challenging due to the small gene panel, but it is a crucial step for downstream analyses. Many good reference-based cell type annotation tools have been developed for single-cell RNA sequencing and sequencing-based spatial transcriptomics data.

Library size confounds biology in spatial transcriptomics data.

Spatial molecular data has transformed the study of disease microenvironments, though, larger datasets pose an analytics challenge prompting the direct adoption of single-cell RNA-sequencing tools including normalization methods. Here, we demonstrate that library size is associated with tissue structure and that normalizing these effects out using commonly applied scRNA-seq normalization methods will negatively affect spatial domain identification. Spatial data should not be specifically corrected for library size prior to analysis, and algorithms designed for scRNA-seq data should be adopted with caution.

Multifunctional packaging film with sustained release behavior triggered by pH microenvironment for efficient preservation of pork.

To tackle microbial contamination and spoilage during pork storage, transportation, and sales, a novel packaging film with exogenously driven controlled release behavior was designed through the demand-directed preparation of the emulsion of shellac-encapsulated cinnamaldehyde nano-capsules (SNCs) and the ingenious integration of chitosan (CS) film matrix. Among them, the SNCs were synthesized using the solvent exchange method, allowing controlled release of cinnamaldehyde (CA) in response to volatile alkaline substances present in the meat. The electrostatic interaction between SNCs and CS molecules improved the thermal stability, water resistance, tensile strength, and viscosity of the film-forming solution, while the CA in SNCs enhanced UV resistance and antimicrobial properties of the film.

Predictive Modeling Analysis for the Quality Indicators of Matsutake Mushrooms in Different Transport Environments.

Matsutake mushrooms, known for their high value, present challenges due to their seasonal availability, difficulties in harvesting, and short shelf life, making it crucial to extend their post-harvest preservation period. In this study, we developed three quality predictive models of Matsutake mushrooms using three different methods. The quality changes of Matsutake mushrooms were experimentally analyzed under two cases (case A: Temperature control and sealing measures; case B: Alteration of gas composition) with various parameters including the hardness, color, odor, pH, soluble solids content (SSC), and moisture content (MC) collected as indicators of quality changes throughout the storage period.

The Joint Effect of Social Comparison and Social Distance on Evaluation of Intertemporal Choice Outcomes in Event-related Potential Studies.

Intertemporal choice plays a crucial role in our daily lives, influencing decisions related to education, health, consumption, and investment. This research proposes an innovative experimental protocol that examines how social comparison and social distance jointly affect the neural processes involved in outcome assessment for intertemporal choices. The study is based on the theoretical framework of cognitive resource competition.

High-accuracy morphological identification of bone marrow cells using deep learning-based Morphogo system.

Accurate identification and classification of bone marrow (BM) nucleated cell morphology are crucial for the diagnosis of hematological diseases. However, the subjective and time-consuming nature of manual identification by pathologists hinders prompt diagnosis and patient treatment. To address this issue, we developed Morphogo, a convolutional neural network-based system for morphological examination.

Joint association of physical activity and sleep duration with risk of all-cause and cause-specific mortality: a population-based cohort study using accelerometry.

Aims: To investigate the joint association of accelerometer-measured physical activity (PA) and sleep duration with mortality risk.

Methods And Results: A 7-day accelerometer recording was performed on 92 221 participants (age 62.4 ± 7.

Association of accelerometer-measured sleep duration and different intensities of physical activity with incident type 2 diabetes in a population-based cohort study.

Purpose: The aim of the current study was to investigate the association of accelerometer-measured sleep duration and different intensities of physical activity (PA) with the risk of incident type 2 diabetes in a population-based prospective cohort study.

Methods: Altogether, 88,000 participants (mean age = 62.2 ± 7.

Microbial β-glucanases: production, properties, and engineering.

Lignocellulosic biomass, which mainly consists of cellulose and hemicellulose, is the most abundant renewable biopolymer on earth. β-Glucanases are glycoside hydrolases (GHs) that hydrolyze β-glucan, one of the dominant components of the plant cell wall, into cello-oligosaccharides and glucose. Among them, endo-β-1,4-glucanase (EC 3.

ARBUSCULAR MYCORRHIZA-INDUCED KINASES AMK8 and AMK24 associate with the receptor-like kinase KINASE3 to regulate arbuscular mycorrhizal symbiosis in Lotus japonicus.

Arbuscular mycorrhizal (AM) symbiosis is a widespread, ancient mutualistic association between plants and fungi, and facilitates nutrient uptake into plants. Cell surface receptor-like kinases (RLKs) and receptor-like cytoplasmic kinases (RLCKs) play pivotal roles in transmembrane signaling, while few RLCKs are known to function in AM symbiosis. Here, we show that 27 out of 40 AM-induced kinases (AMKs) are transcriptionally upregulated by key AM transcription factors in Lotus japonicus.

Integrated metabolomic and transcriptomic analysis reveal the effect of mechanical stress on sugar metabolism in tea leaves () post-harvest.

Sugar metabolites not only act as the key compounds in tea plant response to stress but are also critical for tea quality formation during the post-harvest processing of tea leaves. However, the mechanisms by which sugar metabolites in post-harvest tea leaves respond to mechanical stress are unclear. In this study, we aimed to investigate the effects of mechanical stress on saccharide metabolites and related post-harvest tea genes.

Validation of a wearable forehead sleep recorder against polysomnography in sleep staging and desaturation events in a clinical sample.

Study Objectives: Wearable sleep recording devices may be a helpful alternative method for polysomnography (PSG) due to their higher accessibility and comfort as well as lower cost, but their validities need to be examined. The aim of this study was to evaluate the accuracy of a novel single-channel, electroencephalography-based wearable forehead sleep recorder (UMindSleep) to assess sleep staging and oxygen desaturation.

Methods: Two hundred and three Chinese adults recruited from a sleep medicine center underwent an overnight study wearing UMindSleep and PSG simultaneously.

Human Endogenous Retroviruses and Toll-Like Receptors.

Human endogenous retroviruses (HERVs) are estimated to comprise ∼8% of the entire human genome, but the vast majority of them remain transcriptionally silent in most normal tissues due to accumulated mutations. However, HERVs can be frequently activated and detected in various tissues under certain conditions. Nucleic acids or proteins produced by HERVs can bind to pattern recognition receptors of immune cells or other cells and initiate an innate immune response, which may be involved in some pathogenesis of diseases, especially cancer and autoimmune diseases.

PbI-TiO Bulk Heterojunctions with Long-Range Ordering for X-ray Detectors.

High-performance X-ray detectors are usually based on single crystals, due to the long-range ordering and hence outstanding electronic properties. On the other hand, bulk heterojunctions (BHJs) that can effectively enhance photogenerated exciton dissociation are widely used for photodetectors. The benefits of both spur investigation into how to combine these two strategies to enhance X-ray detection.

Quinoline-cored Poly(Aryl Ether) Dendritic Organogels with Multiple Stimuli-Responsive and Adsorptive Properties.

Functional supramolecular gel materials have potential applications in sensors, optical switches, artificial antennae, drug delivery and so on. In this paper, quinoline-cored poly(aryl ether) dendritic organogelators were designed, synthesized and fully characterized. The gelation behaviour of the dendritic organogelator was tested in organic solvents, mixed solvents and ionic liquids.

Seasonal expression of cytoplasmic creatine kinase in the epididymal epithelium of .

During hibernation of , sperm mature and are stored in the epididymis. We investigated seasonal changes in the morphology of epithelial cells of the epididymis of and changes in expression of cytoplasmic creatine kinase (CK). We found that the epididymal epithelium proliferates rapidly to form multiple layers from June to September, while the epididymal epithelial cells are arranged in a single layer from October to May.

Neuroprotective and Anti-Amyloid β Effect and Main Chemical Profiles of White Tea: Comparison Against Green, Oolong and Black Tea.

White tea (WT) is one of six tea types originally derived from Fujian Province, China. White tea is known for its health-promoting properties. However, the neuroprotective and anti-aggregatory properties of WT against the hallmark toxic Alzheimer's protein, Aβ have not been investigated.

How you act matters: The impact of coordination on 4-year-old children's reasoning about diverse desires.

Cooperation is generally found to have a more positive impact than competition on children's high-level social understanding (e.g., diverse desires, false beliefs); however, recent evidence has shown that competition could also improve such understanding.

Cooperation, but not competition, improves 4-year-old children's reasoning about others' diverse desires.

Three experiments examined whether cooperation or competition affects 4-year-old children's reasoning about other people's desires-which differed from their own-in a gift selection task. Experiment 1 (N=72) found that children's performance in selecting an adult-preferred gift for an adult experimenter was enhanced by a short period of preceding cooperative, but not competitive or individualistic, play with the experimenter. Experiment 2 (N=24) ruled out the alternative explanation that children resisted satisfying their opponent after competition.

Effect of Subcritical Fluid Extraction on the High Quality of Headspace Oil from Jasminum sambac (L.) Aiton.

Subcritical fluid extraction (SFE), as a novel method, was applied to investigate the yield, quality, and sensory evaluation of headspace oil from Jasminum sambac (L.) Aiton in comparison with petroleum ether extraction (PEE). The results indicated that the yield of the headspace oil using SFE was significantly higher (P < 0.

Microfluidic fabrication of cholesteric liquid crystal core-shell structures toward magnetically transportable microlasers.

We report a magnetically transportable microlaser with cholesteric liquid crystal (CLC) core-shell structure, operating in band-edge mode. The dye doped CLC shells as a water-in-oil-in-water (W/O/W) double emulsion were fabricated by microfluidics. Water-dispersible Fe3O4 magnetic nanoparticles were incorporated in the inner aqueous phase by taking advantage of the immiscibility with the middle CLC oil phase.

An Efficient Extraction Method for Fragrant Volatiles from Jasminum sambac (L.) Ait.

The sweet smell of aroma of Jasminum sambac (L.) Ait. is releasing while the flowers are blooming.

Anger and selective attention to reward and punishment in children.

Anger is a negative emotion associated with approach motivation and may influence children's attention preference. Three experiments examined the effect of anger on the attentional biases accompanying reward versus punishment cues in Chinese 5- and 6-year-olds. Experiment 1 tested children who were prone to report angry feelings in an unfair game.