Alleviation of the Byproducts Formation Enables Highly Efficient Biosynthesis of Rosmarinic Acid in .

Rosmarinic acid as a polyphenolic compound has great values in the pharmaceutical, cosmetic, and food industries. To achieve efficient biosynthesis of rosmarinic acid, the major obstacles such as imbalanced metabolic flux among branching pathways and substrate promiscuity of pathway enzymes should be eliminated. Here, a rosmarinic acid producing strain was constructed by introducing codon optimized d-lactate dehydrogenase gene mutant (), 4-coumarate CoA ligase gene (), and rosmarinic acid synthase gene () into a previously constructed caffeic acid hyper-producer.