Chitosan/Sodium Tripolyphosphate/Sodium Alginate/miRNA-34c-5p Antagomir Scaffolds Promote the Functionality of Rabbit Cranial Parietal Repair.

Purpose: MicroRNA-34c-5p (miR-34c-5p) plays a pivotal role in bone remodeling, yet its therapeutic potential is hindered by challenges such as instability, limited cellular internalization, and immune responses. This study was aimed at developing innovative scaffolds capable of efficiently delivering microRNAs (miRNAs), specifically miR-34c-5p.

Methods: Chitosan (CS)/sodium tripolyphosphate (STPP)/sodium alginate (SA) scaffolds, referred to as CTS scaffolds, were synthesized at a specific ratio and characterized using dynamic light scattering and scanning electron microscopy (SEM).

Sandstorms contribute to the atmospheric microplastic pollution: Transport and accumulation from degraded lands to a megacity.

Surface dust from degraded lands is a major global aerosol source, mobilized by meteorological events like sandstorms. Microplastics (MPs) in dust can be enriched in the atmosphere and transported over long distances to sensitive regions during sandstorms. This study was conducted in a megacity frequently impacted by sandstorms in spring, exploring the influx, characteristics, enrichment mechanism, and transport pathway of sandstorm-derived MPs.

Evaluation of the safety of PD-1/PD-L1 inhibitors for immunotherapy in patients with malignant tumors after COVID-19 infection: A single-center cohort study.

Introduction: An increasing body of evidence suggests a close association between COVID-19 infection and the safety of PD-1/PD-L1 inhibitor therapy in cancer patients. However, the available data concerning these impacts remain limited and occasionally contradictory.

Material And Methods: We conducted a retrospective analysis of cancer patients who received PD-1/PD-L1 inhibitor therapy at the same institution from November 2022 to May 2023.

[Construction of synthetic microbial community and its application in polyhydroxyalkanoate biosynthesis].

Synthetic microbial communities are artificial systems composed of multiple microorganisms with well-defined genetic backgrounds. They are characterized by low complexity, high controllability, and strong stability, thus suitable for industrial production, disease management, and environmental remediation. This review summarizes the design principles and construction methods of synthetic microbial communities, and highlights their application in polyhydroxyalkanoate (PHA) biosynthesis.

Human Exposure to Ambient Atmospheric Microplastics in a Megacity: Spatiotemporal Variation and Associated Microorganism-Related Health Risk.

Microplastics are found in various human tissues and are considered harmful, raising concerns about human exposure to microplastics in the environment. Existing research has analyzed indoor and occupational scenarios, but long-term monitoring of ambient atmospheric microplastics (AMPs), especially in highly polluted urban regions, needs to be further investigated. This study estimated human environmental exposure to AMPs by considering inhalation, dust ingestion, and dermal exposure in three urban functional zones within a megacity.

Periodontal ligament cells-derived exosomes promote osteoclast differentiation via modulating macrophage polarization.

Several studies have demonstrated that exosomes (Exos) are involved in the regulation of macrophage polarization and osteoclast differentiation. However, the characteristics as well as roles of exosomes from human periodontal ligament cells (hPDLCs-Exos) in M1/M2 macrophage polarization and osteoclast differentiation remain unclear. Here, periodontal ligament cells were successfully extracted by method of improved Type-I collagen enzyme digestion.

Potential environmental risks of field bio/non-degradable microplastic from mulching residues in farmland: Evidence from metagenomic analysis of plastisphere.

The plastisphere may act as reservoir of antibiotic resistome, accelerating global antimicrobial resistance dissemination. However, the environmental risks in the plastisphere of field microplastics (MPs) in farmland remain largely unknown. Here, antibiotic resistance genes (ARGs) and virulence factors (VFs) on polyethylene microplastics (PE-MPs) and polybutylene adipate terephthalate and polylactic acid microplastics (PBAT/PLA-MPs) from residues were investigated using metagenomic analysis.

Induction of apoptosis and autophagy by dichloromethane extract from Patrinia scabiosaefolia Fisch on acute myeloid leukemia cells.

Patrinia scabiosaefolia Fisch (PS), a perennial herb belonging to the genus Pinus in the family Pinnacle Sauce, has been previously known for its analgesic, anti-inflammatory, antibacterial, and antitumor properties. However, the specific mechanism behind its antileukemic effect remains unknown. This study focused on the cytotoxicity and potential modes of action of the dichloromethane extract from PS (DEPS) in acute myeloid leukemia (AML) cells.

Microplastics in the atmosphere: Adsorb on leaves and their effects on the phyllosphere bacterial community.

Phyllosphere is the largest interface between the atmosphere and terrestrial ecosystems and serves as a major sink for atmospheric microplastics (MPs). It is also a unique habitat for microbiota with diverse ecological functions. This field study investigated the characteristics of atmospheric MPs adsorbed on leaves with automatic technology, and found their abundance was 3.

Differential fungal assemblages and functions between the plastisphere of biodegradable and conventional microplastics in farmland.

The heterogeneity of plastisphere and soil can lead to variation in microbiome, potentially impacting soil functions. Current studies of the plastisphere have mainly focused on bacterial communities, and fungal communities are poorly understood. Biodegradable and conventional microplastics may recruit specific microbial taxa due to their different biodegradability.

Facile synthesis of multicolor emitting sulfur quantum dots and their applications in light blocking field, anti-counterfeiting and sensing.

Sulfur quantum dots (SQDs) have aroused widespread interest from researchers in a wide range of fields due to their excellent photoluminescent properties. Ethylenediamine, diaminopropane and butanediamine were used as precursor amine raw materials to interact with sublimated sulfur to synthesize SQDs with blue, cyan and green fluorescence emission, respectively. Multicolour emitting SQDs were first prepared via sulfur-amine interactions.

Localized electron-accepted yellow-emission carbon dots encapsulated in UiO-66 for efficient visible-light driven photocatalytic activity.

Metal organic frameworks (MOFs) possess a large surface area, inherent porosity and high crystallinity. Nevertheless, they lack electron acceptors, which limit the exploitation of their photocatalytic properties. Carbon dots (CDs) known for excellent optical properties can serve as localized electron acceptors.

Plastic substrate and residual time of microplastics in the urban river shape the composition and structure of bacterial communities in plastisphere.

The widespread secondary microplastics (MPs) in urban freshwater, originating from plastic wastes, have created a new habitat called plastisphere for microorganisms. The factors influencing the structure and ecological risks of the microbial community within the plastisphere are not yet fully understood. We conducted an in-site incubation experiment in an urban river, using MPs from garbage bags (GB), shopping bags (SB), and plastic bottles (PB).

Platinum-Cobalt Nanowires for Efficient Alcohol Oxidation Electrocatalysis.

The compositions and surface facets of platinum (Pt)-based electrocatalysts are of great significance for the development of direct alcohol fuel cells (DAFCs). We reported an approach for preparing ultrathin PtCo nanowire (NW) catalysts with high activity. The PtCo NW alloy catalysts synthesized by single-phase surfactant-free synthesis have adjustable compositions and (111) plane and strain lattices.

Periodontal ligament fibroblasts-derived exosomes induced by PGE inhibit human periodontal ligament stem cells osteogenic differentiation via activating miR-34c-5p/SATB2/ERK.

Several studies have confirmed that exosomes containing microRNAs (miRNAs) from the aseptic inflammatory microenvironment play an important role in bone remodeling. But the mechanism that induces changes in the osteogenic ability of periodontal ligament stem cells (PDLSCs) is still unclear. In the present study, the osteogenic function of periodontal ligament fibroblasts-derived exosomes induced by PGE on PDLSCs was detected by real-time PCR, alizarin red assay and alkaline phosphatase staining.

Spectrins and human diseases.

Spectrin, as one of the major components of a plasma membrane-associated cytoskeleton, is a cytoskeletal protein composed of the modular structure of α and β subunits. The spectrin-based skeleton is essential for preserving the integrity and mechanical characteristics of the cell membrane. Moreover, spectrin regulates a variety of cell processes including cell apoptosis, cell adhesion, cell spreading, and cell cycle.

Advances in polysaccharide nanocrystals as pharmaceutical excipients.

Polysaccharide nanocrystals (PNs) are attractive pharmaceutical excipients due to their abundant surface hydroxyl groups, high surface charges, prominent mechanical properties, excellent fluidity, and good swelling properties. In this review, we summarize three kinds of PNs, including cellulose nanocrystals (CNCs), starch nanocrystals (SNCs), and chitin nanocrystals (ChNCs). We introduce the applications of PNs as stabilizers, adsorbents, film-forming materials, gel materials, disintegrants, and ointment matrices.