Publications by authors named "Xinting Diao"
The fusion of neurotrophic tyrosine receptor kinase (NTRK) is a novel target for cancer therapy and offers hope for patients with gastric cancer (GC). However, there are few studies on the prevalence and detection methods of NTRK fusions in GC. In this study, we used immunohistochemistry (IHC) as a screening method to select cases for molecular testing and evaluated the effectiveness of IHC, fluorescence in situ hybridization (FISH), and next-generation sequencing (NGS).
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- HER2 expression is crucial for breast cancer treatment, especially for patients with HER2-low tumors, as recent therapies have advanced their options.
- This study investigates whether the HER2 expression in archived breast cancer samples decreases over time and how storage affects the detection accuracy.
- Results indicate that HER2 antigen loss increases with longer storage times, but modifying antigen staining protocols can help recover accurate detection in many cases without introducing false positives.
Background: A marked increase in PD1-positive TFH cells in nodal MZL cases (NMZL) was reported previously and could prompt suspicion for a diagnosis of peripheral T-cell lymphoma (PTCL), especially angioimmunoblastic T-cell lymphoma (AITL).
Case Presentation: To demonstrate that the pitfall might exist not only in NMZL but also in transformed splenic MZL (tSMZL), two NMZL cases (70 y/o female with enlarged left cervical lymph node and 75 y/o male with generalized lymphadenopathy) and one case of tSMZL (47 y/o male with nodal and extranodal involvement) with obvious PD1-positive T-cell hyperplasia were described here. Although all their initial diagnoses were prompted to be AITL, they were comprehensively characterized by clinical features, morphologic, immunophenotypic, clonality, and targeted exosome sequencing (TES) findings.
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- This study investigates how genetic abnormalities can aid in diagnosing angioimmunoblastic T-cell lymphoma (AITL) and identifying reliable prognostic factors.
- Researchers analyzed 53 AITL cases, focusing on morphological features, immunophenotypes, and the presence of certain cells, while also conducting targeted exome sequencing for mutations.
- The findings suggest that genetic mutation data can significantly improve diagnosis and indicate that high-density EBER presence is associated with poorer overall and progression-free survival rates.
Survival benefits or symptom alleviation from immune checkpoint blockade therapy can be seen in microsatellite instability-high (MSI-H) cases. However, genetic heterogeneity within a specific subgroup of MSI-H tumors may be associated with poor response and prognosis. We investigated the molecular changes and microsatellite status of the cases with heterogeneous MMR protein staining by polymerase chain reaction (PCR) and next-generation sequencing (NGS).
View Article and Find Full Text PDFAccurate detection of HER2 expression through immunohistochemistry (IHC) is of great clinical significance in the treatment of breast cancer. However, manual interpretation of HER2 is challenging, due to the interobserver variability among pathologists. We sought to explore a deep learning method to predict HER2 expression level and gene status based on a Whole Slide Image (WSI) of the HER2 IHC section.
View Article and Find Full Text PDFBackground: Adenosquamous carcinoma (ASC)with concurrent gastric carcinoma with lymphoid stroma (GCLS) are extremely rare tumors. There are only limited cases reported in the literature. Epstein-Barr virus (EBV) infection was found in the concomitant GCLS, but none in the ASC.
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- PD-L1 is an important biomarker for immunotherapy in non-small cell lung cancer (NSCLC), but its varying levels in different tumor sites present a challenge for clinical testing.
- A study analyzed PD-L1 expression in 131 metastatic NSCLC cases and 972 intrapulmonary cases, revealing that extrapulmonary tumors generally exhibited higher PD-L1 levels than intrapulmonary tumors.
- Specific metastatic sites, like the liver and adrenal glands, showed significantly elevated PD-L1 positivity, while other sites exhibited similar rates to intrapulmonary samples, suggesting the need for tailored detection strategies in advanced lung cancer.
Article Synopsis
- The study investigates the variability of PD-L1 status within core needle biopsies (CNBs) taken from the same tumor in non-small cell lung cancer samples.
- Analysis of 170 surgical specimens revealed that 56% of PD-L1 positive cases had heterogeneous PD-L1 status among their CNBs.
- The research concludes that the CNB with the highest tumor proportion score (TPS) most accurately reflects the overall PD-L1 status compared to other methods, particularly at 1% and 50% cut-off levels.
ALK-positive histiocytosis (APH) is a newly defined entity with specific histological features and a highly recurrent KIF5B-ALK gene fusion. APH is characterized by clonal proliferation of histiocytes and can present as either systemic or localized. It was first described in infants and then expanded to older children and adults.
View Article and Find Full Text PDFBackground/objective: Acral and cutaneous melanomas are usually difficult to accurately diagnose in the early stage, owing to the similarity in clinical manifestations and morphology with those of dysplastic nevus (DN). In this study, we aimed to evaluate the diagnostic value of four-color fluorescence in-situ hybridization (FISH) probes specific to the RREB1,CCND1,and MYB genes, and centromere of chromosome 6, in distinguishing DN and melanoma.
Methods: Fifty one DN and 58 melanoma cases were collected and tested with four-color FISH.