Comparison of the effect of extraction methods on waste cotton (Gossypium hirsutum L.) flowers: metabolic profile, bioactive components, antioxidant, and α-amylase inhibition.

Background: Waste cotton flowers, as a by-product of cotton cultivation, are enriched with bioactive substances that render them a promising natural source of health-promoting benefits. In this study, ultrasound-assisted extraction (UAE), subcritical water extraction (SWE), and conventional extraction (CE) approaches were applied to extract bioactive compounds from waste cotton flowers, and the metabolic profiles, bioactive components, antioxidants, and α-amylase inhibition of different extractions were systematically analyzed and compared.

Results: It was observed that UAE and CE extracts had similar metabolic profiles compared with SWE.

Genome Editing in Cotton with the CRISPR/Cas9 System.

Genome editing is an important tool for gene functional studies as well as crop improvement. The recent development of the CRISPR/Cas9 system using single guide RNA molecules (sgRNAs) to direct precise double strand breaks in the genome has the potential to revolutionize agriculture. Unfortunately, not all sgRNAs are equally efficient and it is difficult to predict their efficiency by bioinformatics.

Genome-wide identification and expression analysis of stress-associated proteins (SAPs) containing A20/AN1 zinc finger in cotton.

Stress-associated proteins (SAPs) containing the A20/AN1 zinc-finger domain play important roles in response to both biotic and abiotic stresses in plants. Nevertheless, few studies have focused on the SAP gene family in cotton. To explore the distributions and expression patterns of these genes, we performed genome-wide identification and characterization of SAPs in tetraploid Gossypium hirsutum L.