[Determination of quercetin in human serum by liquid chromatography-mass spectrometry].

Objective: To establish LC-MS method to determine quercetin in human serum.

Methods: Serum samples were extracted by acetone- methanol and hydrolyzed by β- glucuronidase. The Agilent Poroshell 120 EC C_(18)( 50 mm × 4.

[Effects of theanine on monoamine neurotransmitters and related genes in cerebral ischemia-reperfusion injury rats].

Objective: To study the effects of theanine on dopamine (DA), 5-hydroxy tryptamine (5-TH) and glutamate receptor 2 (GluR2) mRNA, phospholipase-γ1 (PLC-γ1) mRNA in cerebral ischemia-reperfusion injury rats and explore the mechanism of protective effects of theanine on the induced brain injury by ischemia-reperfusion in rats.

Methods: According to random number table, a total of 56 sprague-dawley rats in SPF grade about six-week old and 100 - 120 grams weighting were divided into five groups according to the body weight levels: model group (n = 12), sham-operation group (n = 8), low theanine group (10 mg/kg), middle theanine group (30 mg/kg) and high theanine group (90 mg/kg). There were 12 rats in each of the theanine group.

[Effects of theanine on cerebral ischemia-reperfusion injury in rats].

Objective: To study the protective effect of theanine on cerebral ischemia-reperfusion injury induced by focal cerebral ischemia in rats.

Method: Sprague-Dawley rats were randomly assigned into five groups: model group, shame (SH) control group and 3 theanine groups (Th-L,Th-M and Th-H). The rats in model and SH groups were given distilled water, and the rats in Th groups were given theanine solution (10, 30 and 90 mg/kg respectively) intragastrically for 15 days.

[Effect of lutein on relieving oxidative stress in mice induced by D-galatose].

Objective: To study the effect of lutein on relieving oxidative stress in the liver of mice induced by D-galactose(D-gal).

Methods: Forty eight Kunming strain mice were randomized into 4 groups: model group, low lutein group (LL 10 mg/(kg x d)), high lutein group (HL 40 mg/(kg x d)) and normal control group. The content of reactive oxygen species (ROS), nitric oxide (NO) and activity of total nitric oxide synthase (TNOS), inducible nitric oxide synthase (iNOS) and mitochondrial Na(+)-K(+)-ATPase, Ca(2+)-ATPase in liver tissue were detected 6 weeks later in the experiment.

[Effects of lycopene on cerebral ischemia-reperfusion injury in rats].

Objective: To study the protective effects of lycopene (LP) on cerebral ischemia-reperfusion injury and oxidative stress in SD rats and the mechanism of them.

Methods: The rats were divided into five groups: normal control group, model control group, sham group and two LP groups (fed with 5 mg/kg bw or 20 mg/kg bw of lycopene daily for 15 days). The model for cerebral ischemia-reperfusion injury was established by middle cerebral artery occlusion (MCAO).

[The effects of lycopene on reactive oxygen species and anoxic damage in ischemia reperfusion injury in rats].

Objective: To study the protective effects of lycopene (LP) on cerebral ischemia-reperfusion injury induced by focal cerebral ischemia and oxidative stress in rats.

Methods: 48 male Sprague-Dawley (SD) rats were randomly assigned into five groups: A (20 mg/kg LP), B (5 mg/kg LP), C (salad oil), D (salad oil) and E (basic feed control). A, B and C groups were given LP or salad oil orally for 15 d, then cerebral ischemia-reperfusion injury was established by middle cerebral artery occlusion (MCAO) and D group was used as fake surgery control.

[Mechanism of protective effects of procyanidins on liver injury induced by alcohol in mice].

Objective: To study the mechanism of protective effects of procyanidins on liver injury model induced by alcohol in mice.

Methods: Mice were divided into 3 groups to set up the model of liver injury induced by alcohol in mice: the group of liver injury induced by alcohol, the group of procyanidins with low doses (100mg/kg), the group of procyanidins with high doses(300mg/kg). Then established normal group of control.

[Effects of grape seed proanthocyanidins extracts on experimental diabetic nephropathy in rats].

Objective: To study the effects of grape seed proanthocyanidins extracts(GSPE) and its mechanism on early renal lesions of diabetic rats.

Methods: Diabetic rats induced by alloxan were given GSPE intragastrically for 6 weeks, then the antioxidative indexes and NO content, NOS activity in kidney and serum were measured, and the renal function indexes were tested as well.

Results: Compared with the diabetic group, the urinary protein/24h, levels of blood urea nitrogen(BUN)and serum creatinine(SCr), creatinine clearance rate(CCr) and the ratio of kidney weight/body weight were decreased, the SOD activity in kidney was raised while MDA content was fall in the GSPE group(high dose), and the differences were all significant.

[Green tea extracts protected against carbon tetrachloride-induced chronic liver damage and cirrhosis].

Objective: Using the carbon tetrachloride liver cirrhosis rat model, the protective effect of the green tea extractive (GTE) on the liver cirrhosis was studied.

Methods: Male SD rats were randomly divided into three groups: normal group, GTE group and cirrhosis group. The GTE group and the cirrhosis group were injected subcutanuously 2 times/wk over 9 weeks with 40% CCl(4).