[Glycoside compounds from Chrysanthemum indicum and their antioxidant activity].

Thirteen glycoside compounds were isolated and purified from 95% ethanol extract of Chrysanthemum indicum by normal and reversed phase silica gels, glucan gel, and high performance liquid chromatography, and their chemical structures were identified by spectroscopic data and physicochemical properties. These compounds were(2S,3R)-2,3-dihydro-3-hydroxymethyl-7-methoxy-2-benzodioxole-5-benzofuranpropanol-10-O-β-D-glucopyranoside(1), 4,6,8-decatriynyl-β-D-glucopyranoside(2), 5-(β-D-glucopyranosyloxy)-2-hydroxy benzoic acid methyl ester(3), citrusin C(4), cuminyl β-D-glucopyranoside(5), benzyl-6-[(2E)-2-butenoate]-β-D-glucopyranoside(6), benzyl β-D-glucoside(7), viridoside(8), phenylethyl β-D-glucopyranoside(9), syringaresinol-4'-O-β-D-glucoside(10), pinoresinol-4'-O-β-D-glucoside(11), isoeucommin A(12), and eucommin A(13). Among them, compound 1 was a new compound.

[Analysis of vocal fold movement and voice onset behavior in patients with laryngopharyngeal reflux based on high speed laryngeal high-speed videoendoscopy].

Patients with Laryngopharyngeal Reflux（LPR） have chronic inflammation of the laryngeal mucosa leading to a high response state in the larynx, which may make the vocal fold movement too fast. This paper discusses the characteristics of vocal fold movement and voice onset by analyzing laryngeal high-speed videoendoscopy in patients with LPR. Forty patients with LPR were enrolled as LPR group.

Explore the Pattern of Biomechanical Alterations in Vocal Fold Scar and Its Objective Quantitative Assessment Method.

Objectives: This research aims to discern the evolving nature of the biomechanical properties of vocal fold scarring by calculating Young's modulus for the vocal fold cover layer, the body layer, and the structure as a whole. The study also investigates the potential of diffusion tensor imaging (DTI) for determining these biomechanical characteristics quantitatively.

Methods: A total of six adult female Beagles were divided into two groups (A and B groups) for the creation of unilateral vocal fold scar models, each group containing three subjects.

Deciphering the role of post-translational modifications in fanconi anemia proteins and their influence on tumorigenesis.

Fanconi anemia (FA) is an autosomal or X-linked human disease, characterized by bone marrow failure, cancer susceptibility and various developmental abnormalities. So far, at least 22 FA genes (FANCA-W) have been identified. Germline inactivation of any one of these FA genes causes FA symptoms.

[Analysis of vocal fold movement characteristics in patients with laryngeal neurogenic injury].

To analyze the characteristics of vocal fold movement and glottic closure in patients with laryngeal neurogenic injury. A total of 185 patients with vocal fold paralysis diagnosed by laryngeal electromyography as neurogenic damage to cricothyroid muscle, thyreoarytenoid muscle and posterior cricoarytenoid muscle were enrolled, they were divided into unilateral vocal fold paralysis group and bilateral vocal fold paralysis group, respectively, and superior laryngeal paralysis group, recurrent laryngeal nerve paralysis group and vagal nerve paralysis group according to nerve injury. The characteristics of vocal fold movement and glottic closure were analyzed under strobe laryngoscope.

Engineering Assembly of Plasmonic Virus-Like Gold SERS Nanoprobe Guided by Intelligent Dual-Machine Nanodevice for High-Performance Analysis of Tetracycline.

Accurate detection of trace tetracyclines (TCs) in complex matrices is of great significance for food and environmental safety monitoring. However, traditional recognition and amplification tools exhibit poor specificity and sensitivity. Herein, a novel dual-machine linkage nanodevice (DMLD) is proposed for the first time to achieve high-performance analysis of TC, with a padlock aptamer component as the initiation command center, nucleic acid-encoded multispike virus-like Au nanoparticles (nMVANs) as the signal indicator, and cascade walkers circuit as the processor.

Targeted-activation superparamagnetic spherical nucleic acid nanomachine for ultrasensitive SERS detection of lysozyme based on a bienzymatic-mediated in situ amplification strategy.

Lysozyme (LYS) is a widely used bacteriostatic enzyme. In this paper, we built a sensitive and accurate Raman biosensing platform to detect trace amounts of LYS. The method is based on magnetic spherical nucleic acid formed by a combination of LYS aptamer (Apt) and magnetic beads (MBs).

Vocal Vibration Opening Onset Position Abnormality in Patients With Adductor Spasmodic Dystonia by Using High-Speed Endoscopy.

Objective: To analyze vocal fold vibration onset in patients with adductor laryngeal dystonia (ADLD) by analyzing vocal vibration opening onset position (VVOOP).

Study Design: Case-control study SETTING: A voice center.

Methods: Eleven patients with ADLD diagnosed in our voice center were enrolled in the ADLD group.

Polyhedral Au Nanoparticle/MoO Heterojunction-Enhanced Ultrasensitive Dual-Mode Biosensor for miRNA Detection Combined with a Nonenzymatic Cascade DNA Amplification Circuit.

A novel homologous surface-enhanced Raman scattering (SERS)-electrochemical (EC) dual-mode biosensor based on a 3D/2D polyhedral Au nanoparticle/MoO nanosheet heterojunction (PAMS HJ) and target-triggered nonenzyme cascade autocatalytic DNA amplification (CADA) circuit was constructed for highly sensitive detection of microRNA (miRNA). Mixed-dimensional heterostructures were prepared by growth of polyhedral Au nanoparticles (PANPs) on the surface of MoO nanosheets (MoO NSs) via a seed-mediated growth method. As a detection substrate, the resulting PAMS HJ shows the synergistic effects of both electromagnetic and chemical enhancements, efficient charge transfer, and robust stability, thus achieving a high SERS enhancement factor (EF) of 4.

Increased F-FDG Uptake in the Posterior Region of Contralateral Vocal Folds in Beagle Dogs With Unilateral Vocal Fold Immobility Disorders.

Objective: To explore the glycolytic metabolism of contralateral vocal fold compensation by examining the glycolytic metabolism of the posterior region of vocal folds in beagles with unilateral vocal fold immobility disorders and its correlation with acoustic parameters.

Study Design: Prospective animal study.

Setting: Department of Voice METHODS: Ten adult beagles were divided randomly into three groups: a unilateral cricoarytenoid joint dislocation (UCAJD) group (n = 4), a unilateral vocal fold paralysis (UVFP) group (n = 4), and a control group (n = 2).

Using Diffusion Tensor Imaging to Explore the Changes in the Microstructure of Canine Vocal Fold Scar Tissue.

Objective: To apply diffusion tensor imaging (DTI) in measurement of the diffusion characteristics of water molecules in vocal fold scar tissue, combined with the analysis of textural characteristics of collagen fibers in the cover layer of the vocal folds to explore the feasibility of DTI in the qualitative and quantitative diagnosis of vocal fold scars and the evaluation of microstructural changes of vocal fold scar tissue.

Methods: A unilateral injury was created using micro-cup forceps in the left vocal fold of six beagles. The contralateral normal vocal fold was used as a self-control.

The Effects of Hard Voice Onset on Objective Voice Function in Patients With Laryngopharyngeal Reflux.

Objective: Laryngopharyngeal reflux (LPR) causes laryngopharyngeal hypersensitivity and laryngeal muscle hyperfunction, which may result in hard voice onset in patients with LPR. The purpose of this study is to examine the incidence of hard voice onset in patients with LPR and the effects of hard voice onset on objective voice function in patients with LPR.

Methods: Forty patients with confirmed LPR were enrolled in the LPR group, and 40 healthy subjects were enrolled in the non-LPR group.

Munro Pressure Ulcer Risk Assessment Scale in Adult Patients Undergoing General Anesthesia in the Operating Room.

Pressure ulcers are a common complication of immobility and frequently occur in surgical patients. The occurrence of pressure ulcers is affected by many factors, such as operation time and position, anesthesia method, and postoperative nursing. The aim of this study was to investigate the Munro Pressure Ulcer Risk Assessment Scale's value in predicting acute pressure ulcers in general anesthesia patients.

The Influence of Voice Training on Vocal Learners' Supraglottal Activities and Aerodynamic Evaluation.

Objective: Among music majors with only 18 months of training, supraglottal activities and aerodynamic parameters were studied to facilitate understanding of the influence of voice training on characteristics of voice production.

Methods: Twenty-three students at the Xiamen Music School were examined over the course of 18 months of singing training. Only 17 students completed all data collection sessions.

Fanconi anemia proteins participate in a break-induced-replication-like pathway to counter replication stress.

Fanconi anemia (FA) is a devastating hereditary disease characterized by bone marrow failure (BMF) and acute myeloid leukemia (AML). As FA-deficient cells are hypersensitive to DNA interstrand crosslinks (ICLs), ICLs are widely assumed to be the lesions responsible for FA symptoms. Here, we show that FA-mutated cells are hypersensitive to persistent replication stress and that FA proteins play a role in the break-induced-replication (BIR)-like pathway for fork restart.

The Influence of Voice Training on Vocal Learner's Objective Acoustic Voice Components.

Objective: Acoustic parameters of voice were studied in music majors throughout 18 months of training to understand the influence of voice training on voice.

Methods: Twenty-three students from Xiamen Music School between 12 and 15 years old were enrolled. Acoustic examination was performed three times- every 6 months for 18 months.

To Explore the Changes and Differences of Microstructure of Vocal Fold in Vocal Fold Paralysis and Cricoarytenoid Joint Dislocation by Diffusion Tensor Imaging.

Objective: The diffusion characteristics of water molecules were measured in the vocal folds of canines exhibiting unilateral vocal fold paralysis and unilateral cricoarytenoid joint dislocation. These characteristics were used in conjunction with a histological examination of the microstructural changes of vocal fold muscle fibers to explore the feasibility of diffusion tensor imaging (DTI) in distinguishing unilateral vocal fold paralysis and unilateral cricoarytenoid joint dislocation as well as evaluating microstructural changes.

Methods: Ten beagles were randomly divided into three groups: four in the unilateral vocal fold paralysis group, four in the unilateral cricoarytenoid joint dislocation group, and two in the normal group.

Application of 4D-CT Scanning in Differential Diagnosis of Arytenoid Subluxation and Vocal Fold Paralysis.

Objective: To differentiate arytenoid subluxation and vocal fold paralysis by CT cine mode scanning combined with three-dimensional (3D) reconstruction image.

Methods: Seventy-six patients with suspected vocal fold dyskinesia were collected. All patients were examined being asked to inhale deeply and then make "Yi" sound continuously during CT scanning with cine mode.

A Fast and Cost-Effective Transfer Printing of Liquid Metal Inks for Three-Dimensional Wiring in Flexible Electronics.

The fluidity and high electrical conductivity of a liquid metal make it an excellent material for preparing flexible electronics. Here, we established a method utilizing a widespread laser-printing technique to manufacture 3D flexible electronics. Through digital constructing of liquid alloy-philic smooth toner on liquid alloy-phobic rough substrates, we have the liquid metal selectively adhered to the designed pattern.

SMC5/6 acts jointly with Fanconi anemia factors to support DNA repair and genome stability.

SMC5/6 function in genome integrity remains elusive. Here, we show that SMC5 dysfunction in avian DT40 B cells causes mitotic delay and hypersensitivity toward DNA intra- and inter-strand crosslinkers (ICLs), with smc5 mutants being epistatic to FANCC and FANCM mutations affecting the Fanconi anemia (FA) pathway. Mutations in the checkpoint clamp loader RAD17 and the DNA helicase DDX11, acting in an FA-like pathway, do not aggravate the damage sensitivity caused by SMC5 dysfunction in DT40 cells.

Compensatory Movement of Contralateral Vocal Folds in Patients With Unilateral Vocal Fold Paralysis.

Objectives: Previous studies of subjects with unilateral vocal fold paralysis (UVFP) as observed in a positron emission tomography-computed tomography (PET-CT) examination have demonstrated false positive results in the contralateral cricoarytenoid, in which the metabolism may be higher. This area may also be the site of contralateral compensatory movement in these patients. In this study, we compared the adduction speed of the contralateral vocal folds in patients with UVFP and in healthy subjects as measured by the stroboscopic laryngoscope frame rate.

Evaluation of Diffusional Characteristics and Microstructure in Unilateral Vocal Fold Paralysis Using Diffusion Tensor Imaging.

Objective: To investigate the value of diffusion tensor imaging (DTI) in the evaluation of vocal fold tissue microstructure after recurrent laryngeal nerve (RLN) injury.

Methods: Six canines were divided into 2 groups: a unilateral vocal fold paralysis group (n = 4) and a control group (n 2). The RLN was cut in the unilateral vocal fold paralysis group, and no intervention was applied in the control group.

Study on Normal Laryngeal Electromyography of Thyroarytenoid Muscle, Cricothyroid Muscle, and Posterior Cricoarytenoid Muscle.

Objective: The aim of this study was to investigate the physiological activity of intrinsic laryngeal muscle under different functional states of larynx by measuring the normal laryngeal electromyography parameters.

Methods: Laryngeal electromyography (EMG) was performed in 112 patients with unilateral vocal cord movement disorder. The duration and amplitude of the motor unit potential (MUP) of the thyroarytenoid muscle (TA), posterior cricoarytenoid muscle (PCA), and cricothyroid muscle (CT) were measured when patients were asked to make a deep inspiration and phonate /i/.

Quantitative Measurement of the Three-dimensional Structure of the Vocal Folds and Its Application in Identifying the Type of Cricoarytenoid Joint Dislocation.

Objective: The objective of this study was to quantitatively measure the three-dimensional (3D) structure of the vocal folds in normal subjects and in patients with different types of cricoarytenoid dislocation. We will analyze differences in parameters between the groups and also determine if any morphologic parameters possess utility in distinguishing the type and the degree of cricoarytenoid dislocation.

Study Design: This retrospective study was conducted using university hospital data.