Transcriptome Profiling of Developing Testes and First Wave of Spermatogenesis in the Rat.

Spermatogenesis is a complicated course of several rigorous restrained steps that spermatogonial stem cells undergo to develop into highly specialized spermatozoa; however, specific genes and signal pathways, which regulate the amplification, differentiation and maturation of these cells, remain unclear. We performed bioinformatics analyses to investigate the dynamic changes of the gene expression patterns at three time points in the course of the first wave of rat spermatogenesis. Differently expressed genes (DEGs) were identified, and the features of DEGs were further analyzed with GO (Gene Ontology), KEGG (Kyoto Encyclopedia of Genes and Genomes) and Short Time-series Expression Miner (STEM).

[Expression of the MYL2 gene in the development of rat testis tissue].

Objective: To study the expression of the gene of myosin regulatory light chain-2 (MYL2) in the development of rat testis tissue.

Methods: Using real-time PCR and immunohistochemistry, we determined the mRNA transcription level and protein expression of MYL2 in the rat testis.

Results: The mRNA expression of the MYL2 gene changed in an age-dependent manner, reaching the highest value on postnatal day (PND) 2, then dropped rapidly till PND 8, increased slowly on PNDs 10 and 12, decreased on PND 14, rose slightly from PND 15 and rapidly on PNDs 20 and 25, and declined slowly from PND 65.

Hydrothermal growth of ferrous hydroxide terephthalate as a new positive electrode material for supercapacitors.

Ferrous hydroxide terephthalate, Fe(OH)(C8H5O4), is first prepared by an in situ hydrothermal growth method on nickel foam, which can be directly used as the positive electrodes for supercapacitors with superior pseudocapacitive behaviors. It displays high specific capacitance of 5.25 F cm-2 (656.

[Correlation anslysis of sporadic breast cancer and BRCA1 gene plymorphisms in the Han Nationality and the Mongol Nationality of Inner Mongolia Region].

Objective: To study the correlationship between the BRCA1 gene polymorphisms, especially in 2731 loci (rs799917), and sporadic breast cancer in the Han nationality and the Mongol nationality of the Inner Mongolia region.

Methods: Using the prospective study method, 103 cases of patients with sporadic breast cancer (case group) and 103 cases of normal physical examination people (control group) were enrolled. PCR and direct sequencing method were used for analyzing the correlationship of 2731 loci polymorphisms of BRCA1 and sporadic breast cancer in our zone.