Attention Mask R-CNN with edge refinement algorithm for identifying circulating genetically abnormal cells.

Recent studies have suggested that circulating tumor cells with abnormalities in gene copy numbers in mononuclear cell-enriched peripheral blood samples, such as circulating genetically abnormal cells (CACs), can be used as a non-invasive tool to detect patients with benign pulmonary nodules. These cells are identified through fluorescence signals counting by using 4-color fluorescence in situ hybridization (FISH) technology that exhibits high stability, sensitivity, and specificity. When FISH data are analyzed, the overlapping cells and fluorescence noise is a great challenge for identifying of CACs, thereby seriously affecting the efficiency of clinical diagnosis.