The Effect of Bovine Trypsin on the Adhesion and pH of Dental Plaque Biofilms: An In Vitro Study.

Objective: The aim of this study was to investigate the effect of bovine trypsin on the adhesion and pH of dental plaque biofilms.

Methods: A multispecies dental plaque biofilm model and a single-species dental plaque biofilm model were established in vitro. Three groups were tested: (1) blank control group (aseptic ultrapure water); (2) negative control group (1M Tris-HCl buffer, pH = 7.

The effect of bovine trypsin on dental biofilm dispersion: an in vitro study.

To investigate the degradation effect of bovine trypsin on multispecies biofilm of caries-related bacteria and provide an experimental foundation for the prevention of dental caries. Standard strains of S. mutans, S.

An study on the degradation of multispecies biofilm of periodontitis-related microorganisms by bovine trypsin.

To investigate the degradation effect of bovine trypsin on multispecies biofilm of periodontitis-related bacteria and to provide an experimental reference for exploring new methods for controlling biofilms of periodontitis-related microorganisms, the multispecies biofilm of periodontitis-related microorganisms was established. Standard strains of subsp. , , and were co-cultured to form the biofilm.

Optimization of the UDP-Xyl biocatalytic synthesis from Crassostrea gigas by orthogonal design method.

UDP-Xyl, a nucleotide sugar involved in the biosynthesis of various glycoconjugates, is difficult to obtain and quite expensive. Biocatalysis using a one-pot multi-enzyme cascade is one of the most valuable biotransformation processes widely used in the industry. Herein, two enzymes, UDP-glucose (UDP-Glc) dehydrogenase (CGIUGD) and UDP-Xyl synthase (CGIUXS) from the Pacific oyster Crassostrea gigas, which are coupled together for the biotransformation of UDP-Xyl, were characterized.