[Prognostic value of PSA kinetics in locally advanced prostate cancer treated by maximal androgen blockade combined with brachytherapy].

Objective: To evaluate the effect of post-treatment PSA kinetics on the prognosis of prostate cancer (PCa).

Methods: We retrospectively reviewed the clinical data of 114 cases of locally advanced PCa treated by maximal androgen blockade (MAB) combined with brachytherapy, and analyzed the association of the changes in PSA kinetics with the prognosis of the patients.

Results: The median survival time of the patients was 81 (15 - 144) months, with 1-, 3- and 5-year survival rates of 91.

[Long-term outcomes of prostate cancer patients treated with combined brachytherapy and maximal androgen blockade].

Objective: To explore the prognostic factors of prostate cancer (PCa) patients and evaluate the effect of brachytherapy on survival time.

Methods: A total of 289 PCa were recruited to collect their clinical and survival data. And their possible prognostic factors were analyzed.

Cells susceptible to epithelial-mesenchymal transition are enriched in stem-like side population cells from prostate cancer.

Accumulating evidence suggests that epithelial-mesenchymal transition (EMT) acts as an important factor for the promotion of tumor progression. Strategies for suppressing EMT remain the subject of ongoing research. In the present study, fluorescence-activated cell sorting (FACS) was used to isolate side population (SP) cells from human prostate cancer (PCa) cell lines and xenograft tissues.

[Epithelial-mesenchymal transition of prostate cancer: cancer stem cells or bulk cancer cells].

Objective: To test the hypothesis that epithelial-mesenchymal transition (EMT) of prostate cancer is most likely to occur in cancer stem cells (CSC).

Methods: The isolation of CSC from LNCaP cell line was performed by flow cytometry based on side-population (SP) phenotype. After SP sorting, LNCaP/SP and LNCaP/NSP were used for further transfection of hypoxia-inducible factor-1α (HIF-1α).

[Sorting and identification of cancer stem cells in human prostate cancer cell lines].

Objective: To sort and identify side population (SP) cancer stem cells (CSC) in human prostate cancer (PCa) cell lines.

Methods: Stem-like cells were isolated from five PCa cell lines Du145, IA8, LNCaP, TSU-Pr and PC-3 using FACS based on CD133+ CD44+ immunophenotype and SP in Hoechst staining. The in vitro growth pattern and tumorigenicity of SP stem cells were verified by soft agar colony-formation trial.

[Role of beta-catenin signaling pathway in EMT of human prostate cancer induced by HIF-1alpha].

Objective: Epithelial-mesenchymal transition (EMT) is an important process in tumor development. Several studies suggest that the beta-catenin signal pathway may play an important role in EMT. However, there is no direct evidence showing that this pathway actually determines the EMT induced by exogenous signal.

[Expressions of E-cadherin and beta-catenin in LNCaP and ARCaP cell lines and their significance].

Objective: To observe the expressions of E-cadherin and beta-catenin in LNCaP and ARCaP cell lines and explore their relationship with the metastasis of human prostate cancer.

Methods: The expressions and distribution of E-cadherin and beta-catenin in LNCaP and ARCaP cell lines (IF11 and IA8) were detected by Western blot and immunofluorescent staining.

Results: The expression of E-cadherin was high in LNCaP, but absent in IF11 and IA8, while beta-catenin was expressed highly in IF11 and LA8, but lowly in LNCaP.

[Identification of Wnt/beta-catenin signal pathway in human prostate cancer cell lines and its significance].

Objective: To determine the Wnt/beta-catenin signal pathway in different human prostate cancer cell lines and explore its role in epithelial-mesenchymal transition (EMT) in human prostate cancer.

Methods: We detected the expressions of beta-catenin, t-GSK3beta and p-GSK3beta in several prostate cancer cell lines (LNCaP, C4, C4-2, C4-2B, IF11, IA8, PC-3 and DU145) with different characteristics of epithelial-mesenchymal transition (EMT) by Western blotting.

Results: There were remarkable differences in the expressions of beta-catenin and p-GSK3beta among the cell lines, with beta-catenin and p-GSK3beta highly expressed in LNCaP, C4, C4-2, C4-2B, IF11 and IA8, lowly expressed in PC-3 and DU145, but no difference was observed in the expressions of t-GSK3beta in all the cell lines.