Publications by authors named "Xingyu Mou"

Modifying transmembrane channels is essential for enhancing functionality. Current modification methods often require chemical reactions or protein engineering, which can increase technical complexity and workload. The inner transmembrane region of MscS can bind lipid molecules, referred to as pore lipids, offering an opportunity for fine-tuning channel properties and improving sensing performance.

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Itaconate is a key anti-inflammatory/antibacterial metabolite in pathogen-macrophage interactions that induces adaptive changes in Pseudomonas aeruginosa-exposed airways. However, the impact and mechanisms underlying itaconate metabolism remain unclear. Our study reveals that itaconate significantly upregulates the expression of pyoverdine in P.

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The regulation of carbon metabolism and virulence is critical for the rapid adaptation of pathogenic bacteria to host conditions. In Pseudomonas aeruginosa, RccR is a transcriptional regulator of genes involved in primary carbon metabolism and is associated with bacterial resistance and virulence, although the exact mechanism is unclear. Our study demonstrates that PaRccR is a direct repressor of the transcriptional regulator genes mvaU and algU.

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Point-of-care monitoring of small molecules in biofluids is crucial for clinical diagnosis and treatment. However, the inherent low degree of recognition of small molecules and the complex composition of biofluids present significant obstacles for current detection technologies. Although nanopore sensing excels in the analysis of small molecules, the direct detection of small molecules in complex biofluids remains a challenge.

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Tetracycline repressor (TetR) family regulators (TFRs) are the largest group of DNA-binding transcription factors and are widely distributed in bacteria and archaea. TFRs play vital roles in controlling the expression of various genes and regulating diverse physiological processes. Recently, a TFR protein Pseudomonas virulence regulator A (PvrA), was identified from Pseudomonas aeruginosa as the transcriptional activator of genes involved in fatty acid utilization and bacterial virulence.

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Pseudomonas aeruginosa, a versatile bacterium, has dual significance because of its beneficial roles in environmental soil processes and its detrimental effects as a nosocomial pathogen that causes clinical infections. Understanding adaptability to environmental stress is essential. This investigation delves into the complex interplay of two-component system (TCS), specifically ParRS and CprRS, as P.

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Article Synopsis
  • β--acetylhexosaminidases from the GH20 family are crucial enzymes for modifying oligosaccharides, with significant roles in biology and biotechnology.
  • The study focused on Am2136, a specific β--acetylhexosaminidase from an anaerobic gut bacterium, demonstrating its ability to cleave mucin glycans and highlighting its generalist nature by hydrolyzing β-linkages of various substrates.
  • The research also uncovered that the enzyme's activity is enhanced by nucleotides, suggesting a novel regulatory mechanism linked to structural interactions between enzyme domains, which could facilitate the development of targeted glycan-modifying catalysts.
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Hyperuricemia is involved in the risk of chronic kidney disease (CKD). However, whether urate-lowering therapy (ULT) can influence the progression of kidney function in patients with asymptomatic hyperuricemia is still controversial. We conducted a systematic review and meta-analysis to evaluate the effect of ULT on the progression of kidney function in asymptomatic hyperuricemia patients.

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Current tools for dNTP analysis mainly rely on expensive fluorescent labeling, mass spectrometry or electrochemistry. Single-molecule assay by protein nanopores with an internal diameter of ca. 1-3.

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Article Synopsis
  • - Bacterial type II toxin-antitoxin (TA) systems are important genetic elements that help regulate various physiological processes by producing an antitoxin to neutralize a corresponding toxin.
  • - Research focused on the antitoxin HigA showed that its absence led to increased expression of pathogenic proteins and influenced the expression of virulence-related secretion systems (T3SS and T6SS) by interacting with their promoter regions.
  • - The study suggests that HigA plays a crucial role in bacterial infections, indicating that targeting the HigBA TA system could be a potential strategy for developing new antibacterial treatments.
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Bacterial Mip-like FK506-binding proteins (FKBPs) mostly exhibit peptidyl-prolyl-cis/-isomerase (PPIase) and chaperone activities. These activities are associated with various intracellular functions with diverse molecular mechanisms. Herein, we report the gene-encoded crystal structure of the PAO1's Mip-like protein PaFkbA.

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AlgW, a membrane-bound periplasmic serine protease belonging to the HtrA protein family, is a key regulator of the regulated intramembrane proteolysis (RIP) pathway and is responsible for transmitting the envelope stress signals in The AlgW PDZ domain senses and binds the C-terminal of mis-localized outer membrane proteins (OMPs) or periplasmic protein MucE, leading to catalytic activation of the protease domain. While AlgW is functionally well studied, its exact activation mechanism remains to be elucidated. Here, we show that AlgW is a novel HtrA protease that can be biochemically activated by both peptide and lipid signals.

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Article Synopsis
  • ScPrx1 is a yeast mitochondrial enzyme that protects cells from oxidative stress by utilizing reducing agents like ScTrx3 and glutathione, but its substrate recognition mechanism still needs further investigation.
  • The study determined the structure and oligomeric state of ScPrx1, revealing it exists as a homodimer with specific mutations enhancing or reducing its catalytic activity.
  • The findings suggest that changes in the C-terminal segment and certain loop regions of ScPrx1 are crucial for its catalytic function and its ability to use various reductants.
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MucA and MucB are critical negative modulators of sigma factor AlgU and regulate the mucoid conversion of Pseudomonas aeruginosa. Previous studies have revealed that lipid signals antagonize MucA-MucB binding. Here we report the crystal structure of MucB in complex with the periplasmic domain of MucA and polyethylene glycol (PEG), which unveiled an intermediate state preceding the MucA-MucB dissociation.

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