Carteolol triggers senescence via activation of β-arrestin-ERK-NOX4-ROS pathway in human corneal endothelial cells in vitro.

Carteolol is a commonly-used topical medication for primary open-angle glaucoma. However, long-term and frequent ocular application of carteolol entails its residuals at low concentration in the aqueous humor for a long duration and may exert latent toxicity in the human corneal endothelial cells (HCEnCs). Here, we treated the HCEnCs in vitro with 0.

Ultraviolet B irradiation induces senescence of human corneal endothelial cells in vitro by DNA damage response and oxidative stress.

The human corneal endothelial cells (HCEnCs) play a vital role in the maintenance of corneal transparency and visual acuity. In our daily life, HCEnCs are inevitably exposed to ultraviolet B (UVB) radiation leading to decreases of visual acuity and corneal transparency resulting in visual loss eventually. Therefore, understanding the UVB-induced cytotoxicity in HCEnCs is of importance for making efficient strategies to protect our vision from UVB-damage.

Chitosan/PLGA shell nanoparticles as Tylotoin delivery platform for advanced wound healing.

Wound treatment remains one of the most prevalent healthcare issues. Tylotoin is a skin repair peptide identified from salamander (Tylototriton verrucosus) and exhibits skin wound healing properties. Noticeably, the easy degradation and frequent administration limit its application in wound healing.

Collagen-based biocomposites inspired by bone hierarchical structures for advanced bone regeneration: ongoing research and perspectives.

Bone is a hard-connective tissue composed of matrix, cells and bioactive factors with a hierarchical structure, where the matrix is mainly composed of type I collagen and hydroxyapatite. Collagen fibers assembled by collagen are the template for mineralization and make an important contribution to bone formation and the bone remodeling process. Therefore, collagen has been widely clinically used for bone/cartilage defect regeneration.

Homogeneous deacetylation and degradation of chitin in NaOH/urea dissolution system.

Since being discovered, alkali/urea has been widely used in the dissolution of natural polysaccharides and the preparation of functional materials such as hydrogels, fibers, films and nanoparticles. This work will focus on verifying the structural stability, homogeneous degradation and deacetylation of chitin in alkali-soluble systems. The chitin was dissolved in NaOH/urea solution and stored at different temperature.

Establish an Cell Model to Explore the Impacts of UVA on Human Corneal Endothelial Wound Healing.

Purpose: To provide scientific data for clinical practice in making strategies for accelerating corneal endothelial wound healing, we investigated the impact of UVA on the corneal endothelial wound healing process and the underlying mechanism using an cell model.

Materials And Methods: An cell model for corneal endothelial wound healing was established by scratching the cultured human corneal endothelial cell (HCEnC) confluent layer. Then, we investigated the impacts of UVA irradiation and Ascorbic acid-2-phosphate (Asc-2p) on the wound healing process of the HCEnC model by examining wound-healing index, F-actin rate, Ki-67 rate, and ROS production.

Phenylephrine induces necroptosis and apoptosis in corneal epithelial cells dose- and time-dependently.

In the present study, the toxicity of phenylephrine, a selective α1-adrenergic receptor agonist, in corneal epithelial cells and its underlying mechanisms were investigated using an in vitro model of human corneal epithelial cells (HCEPCs) and an in vivo model of New Zealand white rabbit corneas. The HCEPCs treated with phenylephrine at concentrations from 10% to 0.078125% displayed abnormal morphology, decline of cell viability and elevation of plasma membrane permeability time- and dose-dependently.

Expression pattern of Zinc finger protein 185 in mouse testis and its role in regulation of testosterone secretion.

Zinc finger protein 185 (ZNF185) belongs to the ZNF family and is involved in cell proliferation and differentiation. To the best of our knowledge, the association between ZNF185 and male reproduction is unknown. In the present study, the expression and localization of ZNF185 in mouse testis, as well as its role in testosterone secretion, cell cycle progression and apoptosis of mouse Leydig cells were investigated.

[Expression and localization of zinc finger protein 185 in sperm cells, Leydig cells and Sertoli cells of mouse testis].

Objective To investigate the expression of zinc finger protein 185 (ZNF185) in the sperm cells, Leydig cells and Sertoli cells of the mouse testis. Methods The localization of ZNF185 in the sperm cells, Leydig cells, Sertoli cells and mouse testis tissue was detected by immunofluorescence assay. The mRNA and protein expression levels of ZNF185 in the three kinds of cells were detected by quantitative real-time PCR (qRT-PCR) and Western blotting.