Publications by authors named "Xingrui Liao"
Nanobodies (Nbs) serve as powerful tools in immunoassays. However, their small size and monovalent properties pose challenges for practical application. Multimerization emerges as a significant strategy to address these limitations, enhancing the utilization of nanobodies in immunoassays.
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- Scientists are using special tiny proteins called nanobodies (Nbs) to detect germs like Salmonella better, but they noticed the small size can make it hard for them to work well.
- To solve this, they created bigger nanobodies called bivalent Nbs, which are better at catching Salmonella accurately.
- The new bivalent Nbs worked much better in tests, detecting much smaller amounts of Salmonella and showing great promise for real-world use.
Nanobodies have gained widespread application in immunoassays. However, their small size presents a significant challenge in achieving effective immobilization and optimal sensitivity. Here, we present a novel "one-for-two"-oriented immobilization platform based on an organism-bispecific nanobody (O-BsNb) scaffold, enabling highly sensitive detection of two bacterial pathogens.
View Article and Find Full Text PDFThe exploitation of stable, high-affinity, and low-cost nanoprobes is essential to develop immunoassays for real-time monitoring of foodborne pathogens, so as to safeguard human health. The possible interaction of the Fc fragment of antibodies with spA protein on will result in unexpected interference. To address this consideration, we described herein for the first time the development of nanobodies that by definition are devoid of the Fc fraction.
View Article and Find Full Text PDFRapid separation and identification of Salmonella enteritidis (S. enteritidis) in food is of great importance to prevent outbreaks of foodborne diseases. Herein, by using O and H antigens as targets, an epitope-based bio-panning strategy was applied to isolate specific nanobodies towards S.
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- The study addresses compatibility issues between dyestuffs and antibodies in ultrafast colorimetric lateral flow immunoassays (cLFIA), utilizing a specific dyestuff called gallocyanine (GC).
- It introduces a novel GC-assisted biogenic "potential scalpel" method using clenbuterol (CLE) as a test case, speeding up detection to 30 minutes while maintaining high sensitivity and low costs.
- The technique was successfully demonstrated in detecting CLE in various food products, showcasing its potential for improving food safety monitoring with dyestuffs.
Rapid and sensitive detection of bacterial pathogens present in food and environmental samples is of crucial importance to ensure human health and safety. Here, we present a one-step label-free colorimetric strategy based on M13 bacteriophage-displayed nanobody (phage-Nb) derived from camelid heavy-chain antibodies specific to Vibrio parahaemolyticus (V. parahaemolyticus).
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