Rational development of a human antibody cocktail that deploys multiple functions to confer Pan-SARS-CoVs protection.

Structural principles underlying the composition and synergistic mechanisms of protective monoclonal antibody cocktails are poorly defined. Here, we exploited antibody cooperativity to develop a therapeutic antibody cocktail against SARS-CoV-2. On the basis of our previously identified humanized cross-neutralizing antibody H014, we systematically analyzed a fully human naive antibody library and rationally identified a potent neutralizing antibody partner, P17, which confers effective protection in animal model.

[Construction and toxicity the recombinant SpltMNPV expressing the scorpion toxin gene].

Objective: To develop a high toxic recombinant Spodoptera litura multicapsid nucleopolyhedroviruse (SpltMNPV) insecticide.

Methods: We constructed a recombinant transfer vector that was characterized by disrupting of ecdysterioid UDP-glucosyltransferase (egt) gene and expressing the mature peptide of the Chinese scorpion, B. martensi Karsch (BmK ITal) gene at the control of ie-1 promoter.

Expression of a neurotoxin gene improves the insecticidal activity of Spodoptera litura nucleopolyhedrovirus (SpltNPV).

SpltNPV-C3 is a novel nucleopolyhedrovirus (NPV) screened from 189 wild Spodoptera litura nucleopolyhedrovirus (SpltNPV) clones collected throughout Japan and has high potential insecticidal activity against S. litura. In this study, we constructed two recombinant SpltNPVs, SpltNPV-Δegt and SpltNPV-Δegt-BmK, which were characterized by the disruption of the UDP-glucosyltransferase gene (egt) only and by replacing the egt locus with the Buthus martensi insect-specific toxin gene (BmK ITa1), respectively.