Transcriptomic and metabolomic profiles of Pirata subpiraticus in response to copper exposure.

Copper (Cu) contamination represents a persistent and significant form of heavy metal pollution in agricultural ecosystems, posing serious threats to organisms in current society. Spiders serve as crucial biological indicators for assessing the impact of heavy metals-induced toxicity. However, the specific molecular responses of spiders to Cu exposure and the mechanisms involved are not well understood.

Reversibility and safety of KISS1 metastasis suppressor gene vaccine in immunocastration of ram lambs.

Objective: The aim of this study was to investigate the reversibility and safety of KISS1 metastasis suppressor () gene vaccine in immunocastration.

Methods: Six eight-week old ram lambs were randomly divided into vaccinated and control groups. The vaccine (1 mg/ram lamb) was injected at weeks 0, 3, and 6 of the study.