Comparative Analysis of the Chloroplast Genomes of the (Styracaceae) Species: Providing Insights into Molecular Evolution and Phylogenetic Relationships.

is a member of the Styracaceae family, which is well-known for its remarkable ornamental and medicinal properties. In this research, we conducted comparative analysis of the chloroplast genomes from four samples of representing . The results demonstrated that the chloroplast genome of four samples ranging from 157,103 bp to 158,357 bp exhibited a typical quadripartite structure, including one large single-copy (LSC) region (90,131 bp to 90,342 bp), one small single-copy (SSC) region (18,467 bp to 18,785 bp), and two inverted repeat regions (IRs) (24,115 bp to 24,261 bp).

Intraspecific Differentiation of (Styracaceae) as Revealed by Comparative Chloroplast and Evolutionary Analyses.

is a medicinal and ornamental shrub belonging to the Styracaceae family. To explore the diversity and characteristics of the chloroplast genome of , we conducted sequencing and comparison of the chloroplast genomes of four naturally distributed . The results demonstrated that the four chloroplast genomes (157,914-157,962 bp) exhibited a typical quadripartite structure consisting of a large single copy (LSC) region, a small single copy (SSC) region, and a pair of reverse repeats (IRa and IRb), and the structure was highly conserved.

Photo-induced C(sp)-H difluoroalkylation of anilines.

A photoinduced protocol for the direct difluoroalkylation of C(sp)-H bonds in anilines under catalyst-free reaction conditions is presented. This transformation is characterized by a wide substrate scope, mild reaction conditions, and operational simplicity, and could serve as an alternative tool to established methods for the synthesis of difluoroalkylated anilines. Mechanistic studies suggest the formation of an electron-donor-acceptor (EDA) complex between anilines and difluoroalkyl bromides in this reaction.

Photoinduced Borylation of the Inert C(sp)-O Bond of Alkyl Heteroaryl Ethers.

A photoinduced reductive C-O borylation of alkyl heteroaryl ethers with very negative reduction potential in the presence of 4-dimethylaminopyridine (DMAP) and bis(catecholato)diborane(Bcat) was developed. Despite the high reducing power, various substrates with liable functional groups were well-tolerated as well as ethers derived from natural products and medicinal-relevant compounds. Mechanistic investigation implied that an intra-single electron transfer process in an electron donor-acceptor complex formed from ethers with the adduct of Bcat and DMAP should be involved.

Evaluation of Nonviral and Vector in Functions of CD19chimeric Antigen Receptor T Cells and Their Antitumor Activity for CD19 Tumor Cells.

Nonviral transposon (PB) and (LV) vectors have been used to deliver chimeric antigen receptor (CAR) to T cells. To understand the differences in the effects of PB and LV on CAR T-cell functions, a CAR targeting CD19 was cloned into PB and LV vectors, and the resulting pbCAR and lvCAR were delivered to T cells to generate CD19pbCAR and CD19lvCAR T cells. Both CD19CAR T-cell types were strongly cytotoxic and secreted high IFN-γ levels when incubated with Raji cells.

Chemical Cocktail Induces Hematopoietic Reprogramming and Expands Hematopoietic Stem/Progenitor Cells.

Generation of hematopoietic stem/progenitor cells (HSPCs) via cell expansion or cell reprogramming has been widely achieved by overexpression of transcription factors. Herein, it is reported that without introducing exogenous genes, mouse fibroblasts can be reprogrammed into hemogenic cells based on lineage tracing analysis, which further develop into hematopoietic cells, by treatment of cocktails of chemical compounds. The chemical cocktails also reprogram differentiated hematopoietic cells back into HSPC-like cells.

Flexible Slippery Surface to Manipulate Droplet Coalescence and Sliding, and Its Practicability in Wind-Resistant Water Collection.

A flexible slippery membrane (FSM) with tunable morphology and high elastic deformability has been developed by infusing perfluoropolyether (PFPE) into a fluorinated-copolymer-modified thermoplastic polyurethane (TPU) nanofiberous membrane. To immobilize PFPE in TPU matrix, we synthesized a fluorinated-copolymer poly(DFMA-co-IBOA-co-LMA) with low surface energy, high chemical affinity to PFPE, adequate flexibility, and strong physical adhesion on TPU. Upon external tensile stress, the as-prepared FSM can realize a real-time manipulation of water sliding and coalescence on it.

Durable Antibacterial and Nonfouling Cotton Textiles with Enhanced Comfort via Zwitterionic Sulfopropylbetaine Coating.

A rapid, environment-friendly, and cost-effective finishing method has been developed for cotton textiles by using zwitterionic NCO-sulfopropylbetaine as the antibacterial finishing agent through covalent bond. The sulfopropylbetaine-finished cotton textile exhibits durable broad-spectrum antibacterial and nonfouling activity, improved mechanical properties, and enhanced comfort.

Inhibition of RAC1-GEF DOCK3 by miR-512-3p contributes to suppression of metastasis in non-small cell lung cancer.

MicroRNAs are a class of small non-coding RNAs regulating gene expression. In this study, we demonstrated that retinoic acid (RA) treatment increases the expression of miR-512-3p. Overexpression of miR-512-3p inhibited cell adhesion, migration, and invasion in non-small cell lung cancer (NSCLC) cell lines A549 and H1299.

Connexin 31.1 degradation requires the Clathrin-mediated autophagy in NSCLC cell H1299.

Connexins have relative short half-lives. Connexin 31.1 (Cx31.

In situ generation of electrophilic trifluoromethylthio reagents for enantioselective trifluoromethylthiolation of oxindoles.

An organocatalytic asymmetric trifluoromethylthiolation reaction via in situ generation of active electrophilic trifluoromethylthio species involving trichloroisocyanuric acid and AgSCF3 as a practical and easily handled electrophilic SCF3 source for CSP(3)-SCF3 bond formation was developed. Reactions with this one-pot version strategy occurred in good yields and excellent stereoselectivities to access enantiopure oxindoles bearing a SCF3-substituted quaternary chiral center. The straightforward process described here makes use of simple starting materials and proceeds under mild conditions, which will be useful in medicinal chemistry and diversity-oriented syntheses.

[Modification of the method for preparing rabbit liver VX2 tumor model and its MRI findings].

Objective: To improve the method for preparing rabbit VX2 liver tumor model and observe the magnetic resonance imaging (MRI) features of the implanted tumors.

Methods: Sixteen adult New Zealand white rabbits were assigned randomly into 4 equal groups, and VX2 tumor tissues were implanted into the right and left liver lobes with spiral CT guidance. Plain and contrast-enhanced MR scan and pathological analysis were performed in different stages (14, 18, 22 and 26 days) after tumor implantation.