Metabolic Engineering for the Comprehensive Utilization of ,-Dimethylformamide-Containing Wastewater.

, -dimethylformamide is frequently present in industrial wastewater and is environmentally detrimental. The current study aims to assess the utilization and biodegradation of , -dimethylformamide-containing wastewater to lessen the associated environmental load. Results show that addition of wastewater containing , -dimethylformamide to fermentation media enhances cellulase production and facilitates cellulose hydrolysis.

The novel repressor Rce2 competes with Ace3 to regulate cellulase gene expression in the filamentous fungus Trichoderma reesei.

The filamentous fungus Trichoderma reesei is widely used for industrial cellulase production. In T. reesei, cellulase gene expression is tightly controlled by a regulatory network involving multiple transcription factors.

ACE4, a Novel Transcriptional Activator Involved in the Regulation of Cellulase Genes during Growth on Cellulose.

The filamentous fungus Trichoderma reesei is a model strain for cellulase production. Cellulase gene expression in is controlled by multiple transcription factors. Here, we identified by comparative genomic screening a novel transcriptional activator, ACE4 (ctivator of ellulase xpression 4), that positively regulates cellulase gene expression on cellulose in .

cAMP activates calcium signalling via phospholipase C to regulate cellulase production in the filamentous fungus Trichoderma reesei.

Background: The filamentous fungus Trichoderma reesei is one of the best producers of cellulase and has been widely studied for the production of cellulosic ethanol and bio-based products. We previously reported that Mn and N,N-dimethylformamide (DMF) can stimulate cellulase overexpression via Ca bursts and calcium signalling in T. reesei under cellulase-inducing conditions.

Engineering of for enhanced degradation of lignocellulosic biomass by truncation of the cellulase activator ACE3.

Background: The filamentous fungus is a major workhorse employed to produce cellulase, which hydrolyzes lignocellulosic biomass for the production of cellulosic ethanol and bio-based products. However, the economic efficiency of biorefineries is still low.

Results: In this study, the truncation of cellulase activator ACE3 was identified and characterized in classical mutant NG14 and its direct descendants for the first time.