Knockdown of SLC39A14 inhibits glioma progression by promoting erastin-induced ferroptosis SLC39A14 knockdown inhibits glioma progression.

Background: Ferroptosis is a newly classified form of regulated cell death with implications in various tumor progression pathways. However, the roles and mechanisms of ferroptosis-related genes in glioma remain unclear.

Methods: Bioinformatics analysis was employed to identify differentially expressed ferroptosis-related genes in glioma.

Hypermethylation of the -Activated MAPK Signaling Pathway to Promote the Development of Glioma.

Objective: This study aimed to elucidate the molecular mechanism underlying the involvement of abnormal DNA methylation in the development of glioma and identify potential new targets for glioma therapy.

Methods: The GSE79122 chip achieved from the Gene Expression Omnibus (GEO) database containing 69 glioma samples and 9 normal samples was analyzed. Methylation-specific polymerase chain reaction (MS-PCR or MSP), reverse transcription-PCR, and Western blot analysis were used to confirm the methylation level and expression level of the interleukin receptor-associated kinase (3) gene in glioma cells, 36 glioma samples, and the corresponding normal samples.

Stability and osteogenic potential evaluation of micro-patterned titania mesoporous-nanotube structures.

Although titanium dioxide nanotubes (TNTs) had great potential to promote osteogenesis, their weak bonding strength with titanium substrates greatly limited their clinical application. The objective of this study was to maintain porosity and improve the stability of TNT coatings by preparing some micro-patterned mesoporous/nanotube (MP/TNT) structures via a photolithography-assisted anodization technology. The adhesion strength of different coatings was studied by ultrasonic cleaning machine and scratch tester.

Antibacterial and osteogenesis performances of LL37-loaded titania nanopores in vitro and in vivo.

Many studies have shown that the size of nanotube (NT) can significantly affect the behavior of osteoblasts on titanium-based materials. But the weak bonding strength between NT and substrate greatly limits their application.  The objective of this study was to compare the stability of NT and nanopore (NP) coatings, and further prepare antibacterial titanium-based materials by loading LL37 peptide in NP structures.

Development of Reverse Transcription Thermostable Helicase-Dependent DNA Amplification for the Detection of Tomato Spotted Wilt Virus.

A protocol for the reverse transcription-helicase-dependent amplification (RT-HDA) of isothermal DNA was developed for the detection of tomato spotted wilt virus (TSWV). Specific primers, which were based on the highly conserved region of the N gene sequence in TSWV, were used for the amplification of virus's RNA. The LOD of RT-HDA, reverse transcriptase-loop-mediated isothermal amplification (RT-LAMP), and reverse transcriptase-polymerase chain reaction (RT-PCR) assays were conducted using 10-fold serial dilution of RNA eluates.

Characterization of morphology and component of struvite pellets crystallized from sludge dewatering liquor: Effects of total suspended solid and phosphate concentrations.

A lab-scale struvite pellet crystallization system was used to study phosphorus (P) removal and recovery from sludge dewatering liquor (SDL). Influences of total suspended solids (TSS) and phosphate concentrations on P removal as well as the size, morphology, purity, and components of struvite pellets were investigated. The increase in TSS concentration resulted in not only the decreases in phosphate removal efficiency and struvite purity but also the irregular pellet morphology and broken struvite crystals.