Versatile laccase-mimicking enzyme for dye decolorization and tetracyclines identification upon a colorimetric array sensor.

In this study, the laccase-mimicking enzyme MnO/Cu-BDC-His was synthesized by a facile procedure, and was applied in tetracycline antibiotics (TCs) identification and dye degradation. The MnO/Cu-BDC-His nanozymes effectively recognized phenolic hydroxyl groups in TCs and catalyzed the generation of colored oxidation products with different characteristic absorbance peaks at 350 nm, 525 nm and 600 nm. Different TCs mixtures produced different absorbance intensities at the above wavelengths and exhibited cross-color responses.

Dual-signal biosensor based on G-quadruplex/hemin DNAzyme and zinc-doped molybdenum disulfide quantum dots for ultrasensitive detection of tumor biomarker.

Herein, a dual-mode fluorometric and colorimetric biosensor for Pax-5a gene was developed based on zinc-doped molybdenum disulfide quantum dots (Zn-MoS QDs) by coupling exonuclease-assisted recycling amplification and peroxidase-mimic DNAzyme. In the presence of Pax-5a gene, the exonuclease III can cleave the duplexes formed by Pax-5a gene and the hairpin DNA (HP), releasing the output DNA (oDNA). G-rich DNA and magnetic beads (MBs) labeled with capture DNA (cDNA) can hybridize with oDNA to form the MBs-cDNA/oDNA/G-rich DNA sandwich complex.

Nanowire-like phosphorus modulated carbon-based iron nanozyme with oxidase-like activity for sensitive detection of choline and dye degradation.

Choline is mainly supplemented through food intake, lack of choline would result in diseases like liver cirrhosis, hardening of the arteries, or neurodegenerative disorders. The accurate detection of choline is important for human health. Herein, a novel nanowire-like phosphorus/nitrogen co-doped carbon-based iron nanozyme (Fe-NPC) with outstanding oxidase-like activity was synthesized, and the influence of phosphorus doping on oxidase-like activity was explored.

A highly sensitive dual-mode detection platform based on the novel copper/molybdenum bimetallic nanoclusters and Co-Fe layered doubled hydroxide nanozyme for butyrylcholinesterase activity sensing.

Herein, a novel copper/molybdenum bimetallic nanoclusters (Cu/Mo NCs) with intense blue emission were synthesized by using polyvinylpyrrolidone (PVP) as template and ascorbic acid as reducing agent. Owing to the synergistic effect between Cu and Mo, the fluorescence intensity of Cu/Mo NCs was significantly improved about 6-time than monometallic copper nanoclusters. A novel and sensitive ratiometric fluorescence and colorimetric dual-mode sensing platform for monitoring butyrylcholinesterase (BChE) was strategically constructed by the integration of Cu/Mo NCs with excellent optical properties and Co-Fe layered doubled hydroxide (CoFe-LDH) with superior peroxidase-like activity for the first time.

High-performance colorimetric sensor based on PtRu bimetallic nanozyme for xanthine analysis.

The identification and quantification of xanthine are crucial for assessing the freshness and quality of food products, particularly in the seafood industry. Herein, a new approach was developed, involving the in-situ controllable growth of PtRu nanoparticles on graphitic carbon nitride to yield PtRu@CN catalytic materials. By integrating PtRu@CN with the xanthine/xanthine oxidase (XOD) enzyme catalytic system, a nanozyme-enzyme tandem platform was obtained for the quantification analysis of xanthine.

Construction of the fluorescence sensing platform with a bifunctional Cu@MOF nanozyme for determination of alkaline phosphatase and its inhibitor.

In this work, a novel and sensitive fluorescence sensing system for alkaline phosphatase (ALP) was constructed using a bifunctional copper metal-organic framework (Cu@MOF) nanozyme, which had excellent oxidase-mimetic activity and fluorescence properties. Owing to the presence of 2-amino-1,4-benzenedicarboxylic acid (1,4-BDC-NH) ligand, Cu@MOF displays excellent fluorescence performance at 444 nm. Additionally, Cu endows the oxidase-like activity of Cu@MOF, which could trigger p-phenylenediamine (PPD) to be oxidized to a brown product (PPDox) and quench the photoluminescence of Cu@MOF through the inner filtration effect (IFE).

Fabrication of superior laccase-mimicking enzyme with catalytic oxidative and photothermal properties for anti-bacterial and dual-mode glutathione S-transferase monitoring.

A novel laccase mimic enzyme Cu-Mn with excellent photothermal properties was firstly prepared via a combination of hydrothermal and in situ synthesis. Cu-Mn nanozymes could catalyze the typical laccase substrate 2,4-dichlorophenol (2,4-DP) to generate the red quinone imine. Further, loading the MnO nanosheets with photothermal properties, Cu-Mn nanozymes possessed not only excellent laccase catalytic activity, but also high photothermal conversion efficiency.

A novel robust hydrogel-assisted paper-based sensor based on fluorescence UiO-66-NH@ZIF-8 for the dual-channel detection of captopril.

Captopril (CP) is commonly used as an active enzyme inhibitor for the treatment of coronary heart disease, hypertension and angina pectoris. The development of sensitive and efficient method for CP analysis is of great importance in biomedical research. Herein, we fabricated a sensitive and robust hydrogel-assisted paper-based sensor based on fluorescence UiO-66-NH@ZIF-8 and Co, N-doped carbon nanozymes with oxidase-mimicking activity for accurate monitoring of captopril.

Boron-doped g-CN supporting Cu nanozyme for colorimetric-fluorescent-smartphone detection of α-glucosidase.

Background: Due to that the higher activity of nanozymes would bring outstanding performance for the nanozyme-based biosensing strategies, great efforts have been made by researchers to improve the catalytic activity of nanozymes, and novel nanozymes with high catalytic activity are desired. Considering the crucial role in controlling blood glucose level, strategies like colorimetric and chemiluminescence to monitor α-glucosidase are developed. However, multi-mode detection with higher sensitivity was insufficient.

Construction of a dual-signal readout platform for effective glutathione S-transferase sensing based on polyethyleneimine-capped silver nanoclusters and cobalt-manganese oxide nanosheets with oxidase-mimicking activity.

A novel dual-mode fluorometric and colorimetric sensing platform is reported for determining glutathione S-transferase (GST) by utilizing polyethyleneimine-capped silver nanoclusters (PEI-AgNCs) and cobalt-manganese oxide nanosheets (CoMn-ONSs) with oxidase-like activity. Abundant active oxygen species (O) can be produced through the CoMn-ONSs interacting with dissolved oxygen. Afterward, the pink oxDPD was generated through the oxidation of colorless N,N-diethyl-p-phenylenediamine (DPD) by O, and two absorption peaks at 510 and 551 nm could be observed.

A two-dimensional iron-doped carbon-based nanoenzyme with catalase-like activity for the detection of alkaline phosphatase and ascorbate oxidase.

Herein, we successfully synthesized two-dimensional iron-doped carbon-based nanosheets (Fe-N CS) with catalase-like activity through doping Fe into Zn MOF and introducing graphitic CN (g-CN). The interaction of the Fe-N CS with hydrogen peroxide could generated abundant reactive oxygen species (ROS) and further oxidize o-Phenylenediamine (OPD) to 2,3-diaminophenazine (DAP) which has constant fluorescence at 560 nm. Ascorbic acid (AA) could be generated via the hydrolysis reaction between alkaline phosphatase (ALP) and ascorbic acid 2-phosphate (AAP).

Hydrogel-involved portable colorimetric sensor based on oxidase mimic Fe/Co-NC for acetylcholinesterase detection and pesticides inhibition assessment.

Herein, we synthesized a novel N-doped carbon layer encapsulated Fe/Co bimetallic nanoparticles (Fe/Co-NC), which exhibited superior oxidase-like activity due to the facilitation of electron penetration and the formation of metal-nitrogen active sites. Fe/Co-NC could catalyze the oxidation of 3,3,5,5-tetramethylbenzidine (TMB) to blue oxTMB. Acetylcholinesterase (AChE) could catalyze the hydrolysis of thioacetylcholine to produce reducing thiocholine, which prevented TMB from oxidation.

Self-Assembled Integrated Nanozyme Cascade Biosensor with Dual Catalytic Activity for Portable Urease Analysis.

In this work, a novel nanozyme (Cu@Zr) with all-in-one dual enzyme and fluorescence properties is designed by simple self-assembly. A nanozyme cascade sensor with disodium phenyl phosphate (PPDS) as substrate was first established by exploiting the dual enzymatic activities of phosphatase and laccase. Specifically, phosphatase cleaves the P-O bond of PPDS to produce colorless phenol, which is then oxidized by laccase and complexed with the chromogenic agent 4-aminoantipyrine (4-AP) to produce red quinoneimine (QI).

A novel self-assembled dual-emissive ratiometric fluorescent nanoprobe for alkaline phosphatase sensing.

Background: Alkaline phosphatase (ALP) is widely found in various organs and tissues of the human body which could assist in the verification of the presence of various diseases through its content in the blood. In the past few years, many analytical methods for ALP activity assays have been explored. However, a simple and economical method with high sensitivity and specificity also remains great challenge.

A dual-mode sensing system based on carbon quantum dots and Fe nanozymes for the detection of α-glucosidase and its inhibitors.

In this research, a sensitive fluorometric and colorimetric dual-mode sensing platform based on nitrogen-doped carbon quantum dots (NCDs) and magnetic Fe nanoparticles with peroxidase-like activity (Fe nanozymes, Fe NZs) was established, and was further applied for the detection of α-glucosidase (α-glu) and its inhibitors. The ⋅OH that produced by HO catalyzed by Fe NZs can oxidize the colorless diammonium 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) (ABTS) to green oxABTS, and a noticeable absorption peak at 417 nm appeared. Simultaneously, oxABTS can quench the fluorescence of NCDs at 402 nm via fluorescence resonance energy transfer (FRET).

Multi-signal aptasensor for thrombin detection based on catalytically active gold nanoparticles and fluorescent silicon quantum dots.

A multi-signal aptasensor for thrombin determination is proposed based on catalytically active gold nanoparticles (AuNPs) and fluorescent silicon quantum dots (SiQDs). Yellow 4-Nitrophenol (4-NP) could be converted to colorless 4-Aminophenol (4-AP) by catalytically active aptamer-modified AuNPs (S1-AuNPs). The SiQDs emitted strong blue fluorescence at 455 nm at the excitation wavelength of 367 nm.

A highly sensitive fluorescence "on-off-on" sensing platform for captopril detection based on AuNCs@ZIF-8 nanocomposite.

Here, a novel fluorescent sensing strategy is established for the detection of captopril (CP) sensitively on the basis of a nanocomposite of gold nanoclusters (AuNCs) and metal-organic framework (AuNCs@ZIF-8). The aggregation-induced emission (AIE) effect will be triggered when AuNCs is encapsulated by metal-organic framework (MOF) which served as a carrier since it limits the molecular motion of AuNCs, and the fluorescence of AuNCs greatly enhanced about 5-time after forming the nanocomposites of AuNCs@ZIF-8. The strong orange-emission at 562 nm was quenched in the presence of mercury ions through dynamic quenching.

Construction of a Label-Free Ratiometric Biosensor Based on Target Recycling Amplification and Hg-ZnSe QDs for Assay of BChE and OPs.

Herein, we constructed a label-free ratiometric fluorescence biosensing strategy for the determination of butyrylcholinesterase (BChE) activity and organophosphorus (OPs) concentration. BChE promoted the hydrolysis of iodized -butyrylthiocholine (BTCh) into a reducing substance thiocholine, which can decompose CoOOH nanosheets (CoOOH NSs) to Co. Subsequently, the single-stranded DNA (ssDNA) on the surface of CoOOH NSs was released.

High performance boron doped peroxidase-like nanozyme Cu/B-NC for detection of epinephrine and catalase.

Herein, a novel peroxidase-like (POD-like) nanozyme Cu/B-NC was synthesized. The Cu and B co-doped nanozyme Cu/B-NC has competitive POD-like activity but negligible oxidase-like (OXD-like) activity, which is proved to partly benefit from the doping of boron atom. The catalytic activity of Cu/B-NC is high with great affinity for TMB and HO and high reaction velocity.

Accurate Capture and Identification of Exosomes: Nanoarchitecture of the MXene Heterostructure/Engineered Lipid Layer.

Recently, exosome detection has become an important breakthrough in clinical diagnosis. However, the effective capture and accurate identification of cancer exosomes in a complex biomatrix are still a tough task. Especially, the large size and non-conductivity of exosomes are not conducive to highly sensitive electrochemical or electrochemiluminescence (ECL) detection.

A sensing platform for on-site detection of glutathione S-transferase using oxidized Pi@Ce-doped Zr-based metal-organic frameworks(MOFs).

The development of point-of-care testing (POCT) for glutathione S-transferase (GST) is an effective way to establish the mechanism of targeted monitoring of cancer chemotherapy drug metabolism. Assays for GST with high sensitivity as well as on-site screening have been urgently required to monitor this process. Herein, we synthesized oxidized Pi@Ce-doped Zr-based metal-organic frameworks (MOFs) by electrostatic self-assembly between phosphate and oxidized Ce-doped Zr-based MOFs.

A dual-signal fluorometric and colorimetric sensing platform based on gold-platinum bimetallic nanoclusters for the determination of β-galactosidase activity.

Herein, a novel dual-signal sensing system for the determination of β-galactosidase (β-Gal) activity was established, which was based on a dual-emission probe assembled from gold-platinum bimetallic nanoclusters (Au-Pt NCs) and rhodamine B. Under the catalysis of β-Gal, 4-nitrophenyl β-D-galactopyranoside (PNPG) was rapidly hydrolyzed to generate p-nitrophenol (PNP), which has an obvious UV absorption peak at 400 nm. The hydrolyzed product PNP can quench the fluorescence of Au-Pt NCs effectively by inner filter effect (IFE), and PNP had no impact on the fluorescence of rhodamine B, which will change the emission intensity ratio of Au-Pt NCs and rhodamine B.

Cascade reaction biosensor based on gold nanocluster decorated iron-cobalt oxide nanosheets as a superior peroxidase mimic for dual-mode detection of α-glucosidase and its inhibitor.

Herein, we have synthesized a novel kind of gold nanoclusters decorated iron-cobalt oxide nanosheets (His-AuNCs@FeCo-ONSs) assembled by electrostatic interaction, which possessed both outstanding peroxidase-like activity and fluorescence property. Taking advantage of our bifunctional hybrid nanozyme and enzyme cascade reactions, a sensitive dual-mode (colorimetric/fluorescent) detection method for α-glucosidase was constructed. The detection limits for α-glucosidase were 2.

A ratiometric fluorescence method based on nitrogen-doped carbon quantum dots for the determination of the activity of alkaline phosphatase.

Herein, a simple and sensitive ratiometric fluorescence sensing platform to detect alkaline phosphatase (ALP) activity is developed on the basis of yellow fluorescent nitrogen-doped carbon quantum dots (YNCDs). The hydrolysis of ascorbic acid 2-phosphate (AAP) into ascorbic acid (AA) is catalyzed by ALP. Then, AA will react with o-phenylenediamine (OPD) to form 3-(1,2-dihydroxyethyl)furo[3,4b]-quinoxaline (QXD) which is a blue fluorescent quinoxaline derivative with emission at 435 nm in the presence of Cu.

Co, N co-doped porous carbon-based nanozyme as an oxidase mimic for fluorescence and colorimetric biosensing of butyrylcholinesterase activity.

A Co, N co-doped porous carbon-based nanozyme (Co-N-C nanozyme) has been fabricated. Taking advantages of the excellent oxidase catalytic activity and significant stability of Co-N-C nanozyme, we propose a fluorescence and colorimetric system based on Co-N-C nanozyme and red-emitting carbon quantum dots (RCDs) for butyrylcholinesterase (BChE) sensing. As the chromogenic substrate 3,3',5,5'-tetramethylbenzidine (TMB) was catalyzed and oxidized by Co-N-C nanozyme, the generated oxTMB had a new absorption peak at 652 nm, which resulted in the significant quenching of the fluorescence of the carbon quantum dots at 610 nm.