Effects of TolC on the pathogenicity of porcine extraintestinal pathogenic .

Extraintestinal pathogenic (ExPEC) is a well-known critical pathogenic zoonosis that causes extraintestinal infections in humans and animals by affecting their immune organs. Recently, research on the outer membrane protein of , tolerant colicin (TolC), a virulent protein in the formation of the ExPEC efflux pump, has been an attractive subject. However, the pathogenic mechanisms remain unclear.

EPA and DHA confer protection against deoxynivalenol-induced endoplasmic reticulum stress and iron imbalance in IPEC-1 cells.

This study assessed the molecular mechanism of EPA or DHA protection against intestinal porcine epithelial cell line 1 (IPEC-1) cell damage induced by deoxynivalenol (DON). The cells were divided into six groups, including the CON group, the EPA group, the DHA group, the DON group, the EPA + DON group and the DHA + DON group. RNA sequencing was used to investigate the potential mechanism, and qRT-PCR was employed to verify the expression of selected genes.

Kinetics of changes in gene and microRNA expression related with muscle inflammation and protein degradation following LPS-challenge in weaned piglets.

To test the dynamic changes of the expression of genes and microRNA in the gastrocnemius muscle after LPS challenge, 36 piglets were assigned to a control group (slaughtered 0 h after saline injection) and LPS groups (slaughtered at 1 h, 2 h, 4 h, 8 h, and 12 h after LPS treatment, respectively). After LPS treatment, the mRNA expression of IL-1β, IL-6, and TNF-α reached maximal levels at 1 h, 2 h, and 1 h, respectively ( < 0.05), and mRNA expression of TLR4, NODs, muscle-specific ring finger 1, and muscle atrophy F-box peaked at 12 h ( < 0.

Analysis of microRNA expression profiles in porcine PBMCs after LPS stimulation.

In the present study, we used microRNA (miRNA) sequencing to discover and explore the expression profiles of known and novel miRNAs in 1000 ng/ml LPS stimulated for 8 h vis-à-vis non-stimulated (i.e. control) PBMCs isolated from the blood of healthy pigs.

[Preparation of a novel graphite electrode and its effect on phenol degradation].

In order to find an effective, stable, environmentally compatible electrode for the elimination of organic pollutants, preparation of a novel graphite electrode (NGE) was explored using graphite, epoxy resin, curing agent and acetone as materials. Water, ethanol aqueous solution, acetone, ultrasound-acetone and electrochemical method were used to pretreat the electrode. The first three methods obtained poor effect.

Adsorption of p-nitroaniline from aqueous solutions onto activated carbon fiber prepared from cotton stalk.

Activated carbon fiber prepared from cotton stalk was used as an adsorbent for the removal of p-nitroaniline (PNA) from aqueous solutions. Liquid phase adsorption experiments were conducted and the maximum adsorptive capacity was determined. The effect of experimental parameters such as pH, salinity and temperature on the adsorption was studied.

Equilibrium, kinetic and thermodynamic studies on the adsorption of 2-nitroaniline onto activated carbon prepared from cotton stalk fibre.

Activated carbon prepared from cotton stalk fibre has been utilized as an adsorbent for the removal of 2-nitroaniline from aqueous solutions. The influence of adsorbent mass, contact time and temperature on the adsorption was investigated by conducting a series of batch adsorption experiments. The equilibrium data at different temperatures were fitted with the Langmuir, Freundlich, Tempkin, Redlich-Peterson and Langmuir-Freundlich models.

[Diterpenoids from bulbus of Fritillaria monanth].

To study the chemical constituents of Fritillaria monanth Migo, the constituents were separated and purified by column chromatography on silica gel, and the structures were identified by NMR, MS spectral data. Six compounds were isolated and identified as ent-kauran-15-en-17-ol (I), entkauran-15-en-3alpha, 17-diol (II), fritillaziebinol (III), ent-kauran-16a, 17-diol (IV), ent-kauran-3alpha, 16alpha,17-triol (V), ent-16,17-epoxy-kauran-3alpha-ol (VI). All the compounds were isolated from this plant for the first time, and VI is named as ent-16,17-epoxy-kauran-3alpha-ol, which is a new compound.

[Progress on electrokinetic remediation and its combined methods for POPs from contaminated soils].

Electrokinetic remediation (EK) and its combined methods are emerging technologies to remove POPs from contaminated soils in situ in last decade. This paper reviews the five major kinds of electrokinetic remediation methods for POPs from contaminated soils: (1) Classic electrokinetic method; (2) Electrokinetic-Fenton method; (3) Electrokinetic remediation combining with bioremediation method; (4) Electrokinetic remediation combining with surfactants/cosolvents method; (5) Electrokinetic remediation combining with ultrasonic method. The basic principle, characteristics, application areas and research developments of those five methods are discussed respectively.