Integrated global analysis in spider flowers illuminates features underlying the evolution and maintenance of C photosynthesis.

The carbon concentrating mechanism-C photosynthesis-represents a classic example of convergent evolution, but how this important trait originated and evolved remains largely enigmatic. The spider flower is a valuable leafy vegetable crop and medicinal plant that has also been recognized as a C model species. Here we present a high-quality chromosome-scale annotated genome assembly of through a combination of Oxford Nanopore Technology (ONT), HiFi and Hi-C technology.

A new method based on dispersive liquid-liquid microextraction for fat-soluble vitamin determination in serum by LC-MS/MS.

A green and inexpensive pretreatment known as dispersive liquid-liquid microextraction (DLLME) was developed in this assay coupled with the LC-MS/MS method for routine analysis of fat soluble vitamins (FSVs). The technique was performed with methanol as the dispersive solvent and dichloromethane as the extraction solvent. The extraction phase containing FSVs was evaporated to dryness and reconstituted in a mixture of acetonitrile and water.

An intrinsically disordered region-containing protein mitigates the drought-growth trade-off to boost yields.

Drought stress poses a serious threat to global agricultural productivity and food security. Plant resistance to drought is typically accompanied by a growth deficit and yield penalty. Herein, we report a previously uncharacterized, dicotyledon-specific gene, Stress and Growth Interconnector (SGI), that promotes growth during drought in the oil crop rapeseed (Brassica napus) and the model plant Arabidopsis (Arabidopsis thaliana).

Stress-induced higher vein density in the C3-C4 intermediate Moricandia suffruticosa under drought and heat stress.

The evolution of C4 photosynthesis involved multiple anatomical and physiological modifications, yet our knowledge of the genetic regulation involved remains elusive. In this study, systematic analyses were conducted comparing the C3-C4 intermediate Moricandia suffruticosa and its C3 relative Brassica napus (rapeseed). We found that the leaves of M.

Genome-Wide Identification and Evolutionary Analysis of the Fruit-Weight 2.2-Like Gene Family in Polyploid Oilseed Rape ( L.).

The fruit-weight 2.2 () locus, which was first described in tomato, is known for controlling up to 30% of fruit mass. The functions of its homologs, the FW2.

Three BnaIAA7 homologs are involved in auxin/brassinosteroid-mediated plant morphogenesis in rapeseed (Brassica napus L.).

BnaIAA7 crosstalk with BR signaling is mediated by the interaction between BnaARF8 and BnaBZR1 to regulate rapeseed plant morphogenesis. Auxin (indole-3-acetic acid, IAA) and brassinosteroids (BRs) are essential regulators of plant morphogenesis. However, their roles in rapeseed have not been reported.

Important photosynthetic contribution of silique wall to seed yield-related traits in Arabidopsis thaliana.

In plants, green non-foliar organs are able to perform photosynthesis just as leaves do, and the seed-enclosing pod acts as an essential photosynthetic organ in legume and Brassica species. To date, the contribution of pod photosynthesis to seed yield and related components still remains largely unexplored, and in Arabidopsis thaliana, the photosynthetic activity of the silique (pod) is unknown. In this study, an Arabidopsis glk1/glk2 mutant defective in both leaf and silique photosynthesis was used to create tissue-specific functional complementation lines.

Genome-Wide Association Study Reveals Candidate Genes for Control of Plant Height, Branch Initiation Height and Branch Number in Rapeseed ( L.).

Plant architecture is crucial for rapeseed yield and is determined by plant height (PH), branch initiation height (BIH), branch number (BN) and leaf and inflorescence morphology. In this study, we measured three major factors (PH, BIH, and BN) in a panel of 333 rapeseed accessions across 4 years. A genome-wide association study (GWAS) was performed via Q + K model and the panel was genotyped using the 60 k Infinium SNP array.

Identification of stable QTLs for seed oil content by combined linkage and association mapping in Brassica napus.

Seed oil content is an important agricultural trait in rapeseed breeding. Although numerous quantitative trait locus (QTL) have been identified, most of them cannot be applied in practical breeding mainly due to environmental instability or large confidence intervals. The purpose of this study was to identify and validate high quality and more stable QTLs by combining linkage mapping and genome-wide association study (GWAS).

Natural variation in ARF18 gene simultaneously affects seed weight and silique length in polyploid rapeseed.

Seed weight (SW), which is one of the three major factors influencing grain yield, has been widely accepted as a complex trait that is controlled by polygenes, particularly in polyploid crops. Brassica napus L., which is the second leading crop source for vegetable oil around the world, is a tetraploid (4×) species.

Effects of specific organs on seed oil accumulation in Brassica napus L.

Seed oil content is an important agricultural characteristic in rapeseed breeding. Genetic analysis shows that the mother plant and the embryo play critical roles in regulating seed oil accumulation. However, the overwhelming majority of previous studies have focused on oil synthesis in the developing seed of rapeseed.