Dynamic changes in the hormones of black-necked cranes during reproduction.

Black-necked cranes () are national first-level protected wild animals in China. Artificial breeding has been adopted by many zoos and reserves to achieve conservation of black-necked cranes, but the breeding rate of the species in cages is low. This study used non-invasive methods combined with behavioural observations to investigate changes in sex hormones and glucocorticoid metabolites in the droppings of black-necked cranes during the breeding cycle, with the results showing that (i) levels of estradiol and testosterone in black-necked cranes increased significantly when they entered the breeding period, and these levels could be used as an important physiological indicator to effectively monitor the physiological status of females and males during the reproductive period, thus providing a theoretical basis for the timing of semen collection; (ii) the level of progesterone in the mid-reproduction stage was significantly higher than that in other stages in female black-necked cranes after successful mating, and this level could be an effective indicator of the mating status of female black-necked cranes; (iii) droppings' glucocorticoid metabolites in the breeding period showed different dynamics between paired and singly caged black-necked cranes, indicating that the physiological phenomenon of reproduction could result in a certain amount of physiological burden on black-necked cranes.

Properties of Slurry Shield Tunnel Sand and Its Application in Large Flow Concrete.

The amount of Slurry shield tunnel slag (SSTS) from the Beijing East Sixth Ring Road renovation project is about 3 million m, and it is mainly fine and silt sand. In order to realize its resource utilization, the properties of SSTS and the performance of concrete with strength grades from C30-C60, which used the mixed sand compound with SSTS and Coarse Manufactured Sand (CMS) as a fine aggregate, were investigated. The results showed that SSTS' fineness modulus is 1.

miR-142-5p in Bone Marrow-Derived Mesenchymal Stem Cells Promotes Osteoporosis Involving Targeting Adhesion Molecule VCAM-1 and Inhibiting Cell Migration.

Osteoporosis is a systemic bone metabolic disease that is highly prevalent in the elderly population, particularly in postmenopausal women, which results in enhanced bone fragility and an increased susceptibility to fractures. However, the underlying molecular pathogenesis mechanisms still remain to be further elucidated. In this study, in a rat ovariectomy- (OVX-) induced postmenopausal osteoporosis model, aberrant expression of a microRNA miR-142-5p and vascular cell adhesion molecule 1 (VCAM-1) was found by RNA sequencing analysis and qRT-PCR.

Downregulation of microRNA-31 inhibits proliferation and induces apoptosis by targeting in human keloid.

microRNAs (miRNAs) play a pivotal role in the regulation of cell proliferation and apoptosis in keloid scarring. Integrative analysis of the previous miRNA microarray revealed miRNA-31 was among the most frequently altered miRNAs in keloid and hypertrophic scar. Using qRT-PCR, we further validated miRNA-31 was increased in keloid tissues and keloid-derived fibroblasts.