[Effects of glucocorticoids on tuberculous pleural effusion: experiment with rats].

Objective: To explore the effects of systemic glucocorticoid treatment on tuberculous pleural effusion.

Methods: Ninety Wistar rats were intrapleurally injected with 0.03 mg of standard human Mycobacterium tuberculosis to establish models of tuberculous pleural effusions and then were randomly divided into 2 equal groups both without anti-tuberculosis treatment: glucocorticoids group (GG) undergoing intramuscular injection of 0.

[Analysis on the current situation of Mycobacteria other than tuberculosis during 1994-2003 in the old city area of Guangzhou].

Objective: To analyze the current situation and trend of Mycobacteria other than tuberculosis (MOTT) in the old city area of Guangzhou and to provide information for diagnosis, treatment and policy on tuberculosis (TB) control in the city.

Methods: Relevant data regarding Mycobacteria culture, species identification and drug-resistance from out-patients under suspicion of having pulmonary tuberculosis seen at our TB and Pulmonary Tumor Control Institute, was analyzed retrospectively during 1994-2003.

Results: A total number of 12,634 strains of Mycobacteria were isolated and 794 strains were identified as MOTT which accounted for 6.

[Study on species identification of Mycobacteria by gas chromatography analysis of whole-cell fatty acid].

Objective: To evaluate the accuracy and applicability of species identification method of Mycobacteria by applying gas chromatography analysis of whole-cell fatty acid.

Methods: Species identification of 14 reference strains and 727 clinical isolates of Mycobacteria were performed by using MIDI Sherlock Microbial Identification System (MIS)4.0 based on gas chromatography analysis of whole-cell fatty acid, and the results were compared with those of conventional species identification method.

[A study on the model of tuberculous pleurisy and intrapleural inflammatory and immunological responses in rats].

Objective: To develop a rat model of tuberculous pleurisy and to explore the mechanism of intrapleural inflammatory and immunological responses.

Methods: Fifty Wistar rats were injected intrapleurally with 0.03 mg of standard human mycobacterium tuberculous bacilli H37Rv each.