[Expression of miR-140-5p and prediction of its target gene in human mesenchymal stem cells during adipogenic differentiation].

Objective: To screen the differentially expressed miRNAs and their target genes in adipogenic differentiation of human bone marrow mesenchymal stem cells (hMSCs) to better understand the mechanism for regulating the balance between osteoblast and adipocyte differentiation.

Methods: Cultured hMSCs were induced for adipogenic differentiation, and at 0, 7, 14, and 21 days of induction, the cells were examined for miRNA and mRNA expression profiles using miRNA chip and transcriptome sequencing (RNA-seq) techniques. Correlation analysis was carried out for the miRNAs and mRNAs of potential interest.

A potential association between Toxoplasma gondii infection and schizophrenia in mouse models.

Schizophrenia is a serious neuropsychiatric disease of uncertain etiology, which causes human mental disorder and affects about 1% of the population. In recently years, some studies showed that some cases of schizophrenia may be associated with Toxoplasma gondii infection. In order to investigate a potential association between Toxoplasma infection and schizophrenia, we investigated the relative clinical symptom of schizophrenia such as learning and memory capability, depression and stereotypy to find some useful information by behavioral test in mouse models.

[Study on the processing technique of enzyme hydrolysis Eupolyphaga sinensis peptide and innunoregulatory effect].

Objective: Using papain to prepare polypeptide from Eupolyphaga sinensis, then study the immune function of polypeptide from Eupolyphaga sinensis in vivo.

Methods: Used hydrolysis degree as index, pH value, enzyme dosage, thermometer reaction time were optimized. Studied the influence of polyeptide on the mice immune functions through mice immune organs index, phagocytic function and the level of IL-2.

[Effects of neonatal isolation stress on hyperlocomotion of complexin II knockout mouse].

The aim of this study was to investigate the influence of neonatal isolation stress on hyperlocomotion in complexin II knockout mouse (Cplx2(-/-)). The mice were randomly divided into 4 groups: Cplx2(-/-) with stress, Cplx2(+/+) with stress, Cplx2(-/-) without stress and Cplx2(+/+) without stress. Isolation stress was employed on the pups of stress groups from the 2nd day after the postnatal to the 21st day.

[Study on purification and property of polysaccharide from Scolopendra subspinipes mutilans].

Objective: To obtain polysaccharide from Scolopendra subspinipes mutilans and study its partial properties.

Methods: A raw polysaccharide was isolated from Chinese medicine Scolopendra subspinipes mutilans, and three groups were isolated by DEAE-52. The thin layer chromatography and gel filtration chromatography were used to detect the main monosaccharide composition and molecular weight of the group I.