[Seroepidemiological survey of infection among employees in a pork food processing enterprise].

Objective: To understand the situation of seroepidemiology of infection among employees in a pork food processing enterprise in Dongtai City.

Methods: Totally 200 employees from a pork food processing enterprise in Dongtai City were selected as the research objects. The venous blood of the selected population was extracted and the IgG antibody against in serum was detected by ELISA, and the results were analyzed.

[Effect of strategy of control and prevention of intestinal nematodiasis in Dongtai City, Jiangsu Province].

Objective: To explore the strategy of intestinal nematodiasis control in Dongtai City, Jiangsu Province.

Methods: The strategy of mass drug administration assisted with health education was applied in Dongtai City, which was a high endemic area of intestinal nematodiasis, and then the health education and promotion assisted with drug administration of focus population and other integrated control measures, such as the improvement of drinking water, lavatories, and environment, was applied to consolidate the achievement.

Results: The infection rate of intestinal nematodes reduced from 79.

[Surveillance and risk assessment system of schistosomiasis in Jiangsu Province. II. Surveillance pattern and effect of Oncomelania hupensis snail status in Dongtai City, Jiangsu Province].

Objective: To explore the method of Oncomelania hupensis snail surveillance in transmission-interrupted regions of schistosomiasis, so as to provide a new pattern for the surveillance of snail status in the transmission-interrupted areas.

Methods: In Dongtai County, north of Jiangsu Province where the transmission of schistosomiasis was interrupted, the surveillance of snail status was performed by means of the village-level general survey, village-level sampling survey, snail survey in key settings of township and county, and snail reporting by residents. In addition, quality-control snail sites were set up for quality control.

A "GC-rich" method for mammalian gene expression: a dominant role of non-coding DNA GC content in regulation of mammalian gene expression.

High mammalian gene expression was obtained for more than twenty different proteins in different cell types by just a few laboratory scale stable gene transfections for each protein. The stable expression vectors were constructed by inserting a naturally-occurring 1.006 kb or a synthetic 0.

[Immunogenicity of single-dose HBsAg-PLGA controlled release microspheres in mice].

Aim: To investigate the level of immune response and the immune mechanism of the single-dose hepatitis B surface antigen (HBsAg)-poly (d, l)-lactide-co-glicolide acid (PLGA) microspheres in BALB/c mice.

Methods: Three kind of HBsAg-PLGA microspheres, HBsAg-PLGA50/50-COOH microspheres, HBsAg-PLGA75/25 microspheres and HBsAg-PLGA50/50 microspheres, were prepared by double emulsion microencapsulation technique used three kinds of PLGA with different L/G ratio. The single-dose of HBsAg-PLGA microspheres was subcutaneously injected into BALB/c mice at the dose of 7.

Pharmaceutical and immunological evaluation of a single-dose hepatitis B vaccine using PLGA microspheres.

The objective of the study was to investigate the feasibility of a single-dose hepatitis B vaccine based on three kinds of poly (D, L)-lactide-co-glicolide acid (PLGA) microspheres. PLGA microspheres loaded with recombinant hepatitis B surface antigen (HBsAg) were formulated using a double emulsion microencapsulation technique. The pharmaceutical characteristics of size, surface morphology, protein loading efficiency, antigen integrity, release of HBsAg-loaded PLGA microspheres and degradation of the polymer in vitro were evaluated.

[Preparation, release and immunogenicity evaluation of HBsAg-PLGA microspheres].

Objective: To investigate microencapsulation technique, release of HBsAg-PLGA microspheres and degradation of the polymer in vitro, and the level of immune response after the single-dose HBsAg-PLGA microspheres subcutaneously injected (sc) to BALB/c mice.

Methods: HBsAg-PLGA microspheres were prepared by double emulsion microencapsulation technique with orthogonal experiments. The pharmaceutical characteristics of size and surface morphology, antigen loading efficiency, release of HBsAg-PLGA microspheres and degradation of the polymer in vitro, and the level of immune response after single sc of PLGA microspheres in BALB/c mice were investigated.

[Transplantation of free latissimus dorsi myocutaneous flaps for craniomaxillofacial reconstruction].

Objective: This clinical study was to improve the surgical treatment to craniomaxillofacial tissue defects.

Methods: Since 1997, eight cases with severe craniomaxillofacial defects were treated using free latissimus dorsi myocutaneous flaps. In the operation, nerve anastomosis was performed.