[Prevalence of Enterobius vermicularis infection of children and its influencing factors in Chongqing City].

Objective: To understand the infection status of Enterobius vermicularis of children aged 3-12 years and its influencing factors in Chongqing City.

Methods: One rural county and 1 urban county were selected as investigation areas. The children aged 3-12 years of 5 towns from 5 different directions of each county were investigated.

[Influence factors of Enterobius vermicularis infection among pupils in Chongqing City].

Objective: To investigate Enterobius vermicularis infection among primary school students and its influence factors in Chongqing.

Methods: Beibei and Changshou were selected as investigated points from October to December 2011. One primary school was randomly chosen from each of the 5 different directions in every investigated point.

[Construction and expression of recombinant plasmid pET28alpha-Sj26GST of Schistosoma japonicum in Escherichia coli BL21].

Objective: To construct and express recombinant plasmid pET28a-Sj26GST of Schistosoma japonicum (Sj) in Escherichia coli BL21 (DE3).

Methods: The total RNA was extracted from Sj adult worms by ultrasound-breaking. The Sj26GST antigen gene was amplified by RT-PCR from the total RNA, and then cloned into prokaryotic expression plasmid pET28alpha and transformed into E.