[Research progress on the molecular pathogenesis of Trichomonas vaginalis].

Trichomonas vaginalis is one of the most common human sexually transmitted pathogens that colonize the urogenital mucosa. This paper reviews those factors in the molecular pathogenesis of the parasite, including cell adhesin, interaction with fibronectin and laminin, G-proteins, pore-forming protein and proteinases.

[In vitro anti-Trichomonas vaginalis effects of a mixture of dihydroartemisinin and metronidazole].

Objective: To observe the effect of a mixture of dihydroartemisinin and metronidazole on ultrastructure of Trichomonas vaginalis trophozoites in vitro for exploring trichomonacidal mechanism of the drug mixture.

Methods: The trophozoites were cultivated with liver extract solution medium that contained 2.5 x 10(6) parasites/ml.

[Protein analysis of Trichomonas vaginalis treated by Pulsatilla chinensis in vitro].

Water extract of Pulsatilla chinensis (PWE) (0.625 mg/ml) was added to the medium for the culture of Trichomonas vaginalis. After 2 h and 4 h treatment, the content and component of soluble proteins in the parasites were analyzed by SDS-PAGE.

[Killing effect of polymorphonuclear neutrophils on Trichomonas vaginalis].

Objective: To study the killing effect of polymorphonuclear neutrophils (PMNs) on Trichomonas vaginalis.

Methods: The vaginal secretion from a patient with vaginitis was incubated in the liver infusion liquid medium to get T. vaginalis.

[Effect of dihydroartemisinin on the ultrastructure of Trichomonas vaginalis trophozoites in vitro].

Objective: To observe the effect of dihydroartemisinin (DHA) on the ultrastructure of Trichomonas vaginalis cultured in vitro.

Methods: The trophozoites of T. vaginalis were cultivated with liver extract solution medium containing 1 mg/ml dihydroartemisinin, and were then observed by scanning and transmission electron microscopes after the treatment for 2.

[Construction of prokaryotic expression vector of the fusion gene IFN-alpha1b/CSP II and expression in E. coli].

Objective: To Construct the prokaryotic expression vector of the fusion gene IFN-alpha1b/CSP II.

Methods: IFN-alpha1b was amplified from the human genomic DNA by PCR and cloned into prokaryotic expression vector pGEX-4T-1. The recombinant plasmid pGEX-4T-1/IFN-alpha1b was constructed.

[Study on surface adhesion protein 33 gene sequence of different Trichomonas vaginalis isolates].

Objective: To study genetic polymorphism of surface adhesion protein 33 (AP33) gene on the seven isolates of Trichomonas vaginalis.

Methods: PCR technique was performed to amplify AP33 gene from the seven isolates, DNA sequences were obtained from the AP33 gene of the isolates and phylogenetic tree was built. Minimal lethal concentrations (MLC) of metronidazole on the isolates were measured in vitro.

[RAPD analysis on different isolates of Trichomonas vaginalis].

Objective: To study genetic polymorphism of DNA on seven isolates of Trichomonas vaginalis.

Methods: The random amplified polymorphic DNA (RAPD) technique was performed to amplify genomic DNA of the seven T. vaginalis isolates, including Beijing 1, Beijing 2, Chengde, Tangshan, Jiujiang 1, Jiujiang 2 and Jiujiang 3.

[Isoenzyme analysis on different isolates of Trichomonas vaginalis].

Objective: To study the biological types on the seven isolates of Trichomonas vaginalis from Beijing, Hebei-Tangshan, Hebei-Chengde and Jiangxi-Jiujiang in the mainland of China.

Methods: The samples were analyzed by PAGE, isoenzyme stain and cluster analysis.

Results: The isoenzyme systems used in the study included MDH, LDH, G-6-PD, PGI and PGM.