Publications by authors named "Xing-Yuan Shi"
Single-cell transcriptomic analysis of radiation-induced lung injury in rat.
Biomol Biomed
September 2024
Radiation-induced lung injury (RILI) frequently occurs as a complication following radiotherapy for chest tumors like lung and breast cancers. However, the precise underlying mechanisms of RILI remain unclear. In this study, we generated RILI models in rats treated with a single dose of 20 Gy and examined lung tissues by single-cell RNA sequencing (scRNA-seq) 2 weeks post-radiation.
View Article and Find Full Text PDFPosttranslational modification dramatically enhances protein complexity, but the function and precise mechanism of novel lysine acylation modifications remain unknown. Chemoresistance remains a daunting challenge to successful treatment. We found that lysine butyrylation (Kbu) is specifically upregulated in chemoresistant tumor cells and tissues.
View Article and Find Full Text PDFAlthough N4-acetylcytidine (ac4C) modification affects the stability and translation of mRNA, it is unknown whether it exists in noncoding RNAs, and its biological function is unclear. Here, nucleotide-resolution method for profiling CTC-490G23.2 ac4C sites and gain- and loss-of-function experiments revealed that N-acetyltransferase 10 (NAT10) is responsible for ac4C modification of long noncoding RNAs (lncRNAs).
View Article and Find Full Text PDFInhibiting the Activity of ABCG2 by KU55933 in Colorectal Cancer.
Recent Pat Anticancer Drug Discov
July 2022
Background: Therapeutic resistance is a frequent problem of cancer treatment and a leading cause of mortality in patients with metastatic colorectal cancer (CRC). Recent insight into the mechanisms that confer multidrug resistance has elucidated that the ATP-binding cassette (ABC) superfamily G member 2 (ABCG2) assists cancer cells in escaping therapeutic stress caused by toxic chemotherapy. Therefore, it is necessary to develop ABCG2 inhibitors.
View Article and Find Full Text PDFArticle Synopsis
- USP22 is linked to poor cancer prognosis, but its specific role in nasopharyngeal carcinoma (NPC) has not been previously studied.
- The study employed various methods, including immunohistochemistry and RT-PCR, to find that USP22 expression is significantly higher in NPC cells compared to non-cancerous cells and tissues.
- Knocking down USP22 in NPC cell lines inhibited their growth and altered the cell cycle, suggesting USP22’s involvement in the AKT/GSK-3/Cyclin signaling pathway and identifying it as a potential target for future cancer treatments.
Article Synopsis
- Higher levels of the transcriptional coactivator p300 were found in nasopharyngeal carcinoma (NPC) tissues compared to normal nasopharyngeal mucosal tissues, indicating its potential role in cancer development.
- The study utilized techniques like RT-PCR, Western blotting, and immunohistochemistry to analyze p300 expression, finding that over 60% of NPC cases exhibited high p300 levels, which correlated with advanced cancer stages and metastasis.
- Ultimately, the research suggests that p300 expression could serve as a significant independent marker for predicting poorer survival outcomes in NPC patients.
Objective: To evaluate the therapeutic effect and toxicity of intensity-modulated radiation therapy (IMRT) or three-dimensional conformal radiotherapy combined with chemotherapy (3-DCRT) with docetaxel and cisplatin in the treatment of locally advanced esophageal carcinoma.
Methods: Sixty patients with locally advanced esophageal carcinoma were randomly assigned in two equal groups to receive IMRT or 3-DCRT, both combined with the chemotherapy with docetaxel and cisplatin. The total dose of radiotherapy was 64 Gy, administered in 30 fractions in 6 weeks.