[Diversity of the Microbial Community in Rice Paddy Soil with Biogas Slurry Irrigation Analyzed by Illumina Sequencing Technology].

In order to explore the variation in soil microbial community diversity in paddy fields with different irrigation periods, we collected in situ rice field soils during different biogas irrigation periods and analyzed the microbial community structures of these soils by high-throughput sequencing. The results showed that as the biogas irrigation period increased, the soil pH decreased gradually, while organic matter, nitrate nitrogen, phosphate, and other nutrients were accumulated. Years of continued biogas irrigation was not conducive to improving rice yields.

Psc-AFP from Psoralea corylifolia L. overexpressed in Pichia pastoris increases antimicrobial activity and enhances disease resistance of transgenic tobacco.

Psc-AFP, isolated from the seeds of Psoralea corylifolia L., is an antimicrobial protein with trypsin inhibitor activity. Its encoding gene was cloned by 3'- rapid amplification of cDNA ends (RACE) combined with Y-shaped adaptor-dependent extension (YADE) method.

Cloning and characterization of a balsam pear class I chitinase gene (Mcchit1) and its ectopic expression enhances fungal resistance in transgenic plants.

A balsam pear (Momordica charantia L.) chitinase (Mcchit1) was purified and sequenced at the N-terminal. The genomic and cDNA coding sequences of Mcchit1 were cloned by rapid amplification of 3' cDNA ends (3'-RACE) and the Y-shaped adaptor dependent extension (YADE) method.

Isolation and characterization of a carboxylic transport protein JEN1 and its promoter from Metarhizium anisopliae.

Based on the flanking sequence of T-DNA of a T-DNA insertion mutant of Beauveria bassiana, T12, the full length cDNA of carboxylic transport protein, designated MaJen1, was cloned from Metarhizium anisopliae. MaJen1 is 1,695 bp long and contained a 1,524 bp ORF which predicted a protein of 508 amino acid. The amino acid sequence of the gene showed 69% and 31% identity to the carboxylic transport protein of Neurospore crassa and Saccharomyces cerevisiae, respectively.

[Cloning and characterization of a homologous gene of plant class V chitinase from balsampear, Momordica charantia Linn].

Balsampear (M. charantia Linn.) is a vegetable crop, highly resistant to pathogens.

[Cloning and characterization of cuticle degrading enzyme CDEP-1 from Beauveria bassiana].

Beauveria bassiana extracellular subtilisin-like serine endoprotease is a potential virulence factor by virtue of its activity against insect cuticles. A cDNA library was constructed using mRNA from mycelia of Beauveria bassiana grown on cuticle/chitin cultures. A cDNA clone of the protease, designated CDEP-1, was isolated from cDNA library.