HPLC-MS analysis of ethanol extract of Corydalis yanhusuo and simultaneous determination of eight protoberberine quaternary alkaloids by HPLC-DAD.

An accurate and simple analysis method by high-performance liquid chromatography (HPLC)-electrospray tandem mass spectrometry (ESI-MS-MS) for the ethanol extract of Corydalis yanhusuo was established in this study. In this method, a C(18) column was used, and the mobile phase was water (0.2% acetic acid, 0.

[Identification and analysis of absorbed components in rat plasma after oral administration of active fraction of Corydalis yanhusuo by LC-MS/MS].

To analyze and identify the constituents in rat plasma after oral administration of the active fraction of Corydalis yanhusuo, a LC-MS/MS method was established. The constituents absorbed into blood, their original crude drugs and their metabolites were identified either by comparing the retention time and mass spectrometry data with that of reference compounds or by mass spectrometry analysis and retrieving the reference literatures. Nine species are the original form in Corydalis yanhusuo, moreover, some metabolites in blood identified as glucuronide were found.

[Studies on chemical constituents in the anti-myocardial ischemia effective fraction of Corydalis yanhusuo].

Objective: To study the chemical constituents in the anti-myocardial ischemia effective fraction of Corydalis yanhusuo.

Methods: Compounds were isolated and purified by a macroporous resin, Sephadex LH-20, ODS column chromatography, and prepared TLC. Their structures were identified by spectral analysis and chemical evidence.

Two new protoberberine quaternary alkaloids from Corydalis yanhusuo.

Two new protoberberine quaternary alkaloids, 13-methyl-palmatrubine (1) and 13-methyl-dehydrocorydalmine (2), were isolated from the tubers of Corydalis yanhusuo W.T. Wang.

[Analysis of chemical constituents of anti-myocardial ischemia fraction of Corydalis yanhusuo].

Objective: To establish the HPLC fingerprint of anti-myocardial ischemia fraction of Corydalis yanhusuo, and to investigate its chemical constituents by LC-MS-MS.

Method: The separation was performed at 30 degrees C on a Dikma Diamonsil C18 column (4.6 mm x 250 mm, 5 microm) by isocratic elution with acetonitrile-water (23:77) (containing 0.