[Effect of Bushen Yanggu Decoction on Multidrug Resistance of Multiple Myeloma Cell Line KM3/BTZ].

Objective: To explore the effect of Bushen Yanggu Decoction (BYD) on drug resistance and proliferation of human multiple myeloma-resistant KM3/BTZ cells.

Methods: Human multidrug-resistant KM3/BTZ cells were established by Bortezomib (BTZ) gradient induction. The effects of commonly used chemotherapeutic drugs and serum containing Bushen Yanggu Decoction (BYD) on the proliferation of KM3 cells and KM3/BTZ cells were detected by MTT assay.

[Effect of siRNA-Interfering β-Catenin Expression on MDR of Human Multiple Myeloma Cell Line].

Objective: To investigate the effecr of siRNA-interfering β-catenin expression on drug-resistance of multiple myeloma cells.

Methods: The multiple myeloma cell line RPMI-8226 was cultured in vitro. The maphalan-resistant cell model was established by concentration gradient ascending of durg, then the drug-resistant cell line was instantaneously transfected with β-catenin siRNA, the sensitivity of RPMI 8226 cells to maphalan was detected by CCK-8 meltod before and after the transfection with siRNA; the mRNA and protein expression of β-catenin was detected by qRT-PCR and Western blot respectively, the apoptosis of cells was detected by flow cytometry.

[Icaritin Reverses Multidrug Resistance of Multiple Myeloma Cell Line KM3/BTZ].

Objective: To investigate the reversing effect of icaritin on multidrug resistance of multiple myeloma cell lines KM3/BTZ and its underlying mechanism.

Methods: KM3/BTZ cells were established by a gradually ascending gradient induction of bortezomib (BTZ). The sensitivities of KM3 and KM3/BTZ cells to 7 chemotherapeutic drugs, the inhibition and reversal effects of icaritin on proliferation and drug-resistance of KM3/BTZ cells were analyzed by MTT.

[Effect of Reclaimed Water on Bacterial Community Composition and Function in Urban River Sediment].

In order to study the effect of reclaimed water on bacterial community composition and function in urban river sediment, the changes of bacteria community diversity, composition and function in Mayu wetland upon the supply of reclaimed water were investigated by a range of sophisticated procedures, including Terminal Restriction Fragment Length Polymorphism(T-RFLP), 16S rRNA clone library technology, and Real-time Quantitative PCR Detecting System(qPCR).The results showed that carbon, nitrogen and phosphorus were major factors driving the variation of bacterial diversity and community structure in river sediment, and the bacteria were gradually recovered after purification in downstream under the effect of artificial wetland. In addition, the bacterial community in reclaimed water outfall was mainly constituted by -Proteobacteria, -Proteobacteria, Bacteroidales and Cyanobacteriain, and -Proteobacteria, Chloroflexi and Spirochaetes were unique groups.