One-step gene knock-out in porcine embryos using recombinant adeno-associated viruses.

Gene-edited pigs have become prominent models for studying human disease mechanisms, gene therapy, and xenotransplantation. CRISPR (clustered regularly interspaced short palindromic repeats)/CRISPR-associated 9 (CRISPR/Cas9) technology is a widely employed tool for generating gene-edited pigs. Nevertheless, delivering CRISPR/Cas9 to pre-implantation embryos has traditionally posed challenges due to its reliance on intricate micromanipulation equipment and specialized techniques, resulting in high costs and time-consuming procedures.

[Composition changes reveal relationship between color and enzymatic reaction of Magnoliae Officinalis Cortex during "sweating" process].

In this study, we employed Q Exactive to determine the main differential metabolites of Magnoliae Officinalis Cortex du-ring the "sweating" process. Further, we quantified the color parameters and determined the activities of polyphenol oxidase(PPO), peroxidase(POD), and tyrosinase of Magnoliae Officinalis Cortex during the "sweating" process. Gray correlation analysis was performed for the color, chemical composition, and enzyme activity to reveal the effect of enzymatic reaction on the color of Magnoliae Officinalis Cortex during the "sweating" process.

Creating rat hepatocyte organoid as an model for drug testing.

Background: Liver organoids have recently been applied as models for liver disease and drug screening, especially when combined with liver-on-a-chip technologies. Compared to hepatocyte-like cells, primary hepatocytes have high functionality but cannot maintain their function when cultured . Mesenchymal stem cells (MSCs) enhance hepatocyte function and maintain hepatocyte metabolism when co-cultured with hepatocytes.

Extremely brilliant GeV γ-rays from a two-stage laser-plasma accelerator.

Recent developments in laser-wakefield accelerators have led to compact ultrashort X/γ-ray sources that can deliver peak brilliance comparable with conventional synchrotron sources. Such sources normally have low efficiencies and are limited to 10 photons/shot in the keV to MeV range. We present a novel scheme to efficiently produce collimated ultrabright γ-ray beams with photon energies tunable up to GeV by focusing a multi-petawatt laser pulse into a two-stage wakefield accelerator.

Efficient generation of relativistic near-single-cycle mid-infrared pulses in plasmas.

Ultrashort intense optical pulses in the mid-infrared (mid-IR) region are very important for broad applications ranging from super-resolution spectroscopy to attosecond X-ray pulse generation and particle acceleration. However, currently, it is still difficult to produce few-cycle mid-IR pulses of relativistic intensities using standard optical techniques. Here, we propose and numerically demonstrate a novel scheme to produce these mid-IR pulses based on laser-driven plasma optical modulation.

Exploration of the structure-activity relationship and druggability of novel oxazolidinone-based compounds as Gram-negative antibacterial agents.

To gain further knowledge of the structure-activity relationship and druggability of novel oxazolidinone-based UDP-3-O-acyl-N-acetylglucosamine deacetylase (LpxC) inhibitors as Gram-negative antibacterial agents, compounds containing the hydrophobic tails with different lengths and terminal substitutions were synthesized and their antibacterial activities against standard and clinically isolated Gram-negative strains were evaluated. We summarized their structure-activity relationships and found that oxazolidinone-based compounds exhibited a narrower antibacterial spectrum compared with threonine-based compounds. Furthermore, we parallelly compared the metabolic stabilities of the compounds with the classic threonine scaffold and the novel oxazolidinone scaffold in liver microsomes.

Ultra-bright γ-ray emission and dense positron production from two laser-driven colliding foils.

Matter can be transferred into energy and the opposite transformation is also possible by use of high-power lasers. A laser pulse in plasma can convert its energy into γ-rays and then e e pairs via the multi-photon Breit-Wheeler process. Production of dense positrons at GeV energies is very challenging since extremely high laser intensity ~10 Wcm is required.

All-optical bright γ-ray and dense positron source by laser driven plasmas-filled cone.

An all-optical scheme for bright γ-rays and dense ee pair source is proposed by irradiating a 10 W/cm laser onto a near-critical-density plasmas filled Al cone. Two-dimensional (2D) QED particle-in-cell (PIC) simulations show that, a dense electron bunch is confined in the laser field due to the radiation reaction and the trapped electrons oscillate transversely, emitting bright γ-rays forward in two ways: (1) nonlinear Compton scattering due to oscillation of electrons in the laser field, and (2) Compton backwardscattering resulting from the bunch colliding with the reflected laser by the cone tip. Finally, the multi-photon Breit-Wheeler process is initiated, producing abundant ee pairs with a density of ∼ 10m.