[Effect of Valeriana jatamansi extract on fecal UPLC-MS/MS metabolomics in rats with diarrheal irritable bowel syndrome].

The purpose of this study was to understand the pharmacodynamic effect of Valeriana jatamansi extract in diarrhea predominant irritable bowel syndrome(IBS-D) rat model induced by maternal separation combined with three kinds of stress, and observe the changes of endogenous metabolites in feces after intervention to find potential biomarkers and related metabolic pathways. The animal model of IBS-D was established by maternal separation combined with restraint, ice swimming and tail clamping. The therapeutic effect of each dose group of V.

[Effects of baldrinal of Valeriana jatamansi on expression of CRF, TPH1 mRNA and 5-HT in rats with irritable bowel syndrome].

This study aimed to investigate the effects of baldrinal of Valeriana jatamansi on the expression of corticotropin releasing factor (CRF) and tryptophan hydroxylase 1 (TPH1) mRNA and levels of 5-hydroxytryptamine (5-HT) in colon of rats with irritable bowel syndrome (IBS), and to explain its therapeutic mechanism on IBS through 5-HT pathway. Fifty-four male SD rats were randomly divided into 6 groups: blank group, model group, baldrinal high, medium and low dose groups, and pinaverium bromide group, n=9 in each group. The IBS rat models were established by using unpredictable chronic stress for 3 weeks followed by 1-hour acute restraint stress (CAS) after 7 days of rest and independent feeding.

Phenolic constituents from Rheum nobile and their antioxidant activity.

Although the rhizomes of Rheum nobile Hook. f. et Thoms (Polygonaceae) are widely used in Tibetan medicine, no previous investigations regarding the biological activities and rarely chemical constituents of this plant have been reported.

[Textual research for Tibetan medicine Qumazi].

Qumazi is a commonly used Tibetan medicine. With a long history, it can be found in the Four Medical Tantras written by gYu-thog rNying-ma Yon-tan mGon-po since the 8th century AD. Qumazi grows in mudflats and fields, including species growing in highlands, lowlands, mountains and farmlands.

[Sequence analysis and identification of a chloroplast matK gene in Rhei Rhizoma from different botanical origins].

Rhei Rhizoma is a Chinese medicine with multiple botanical origins. There is a problem to identify it with conventional methods. To compare the characteristics of chloroplast matK gene sequences of different Rheum species and authenticate inspected species, the matK gene sequences of different species from different origins were amplified, cloned, and sequenced.

[Study on quality specification of Rhizoma et Radix Valeriana Jatamansi].

Objective: To provide scientific basis for the utilization and development of Valeriana jatamansi by setting up the quality control specification of V. jatamansi.

Method: The pharmacognostical methods were applied.

[Determination of complanatoside A in semen Astragali complanati by HPLC].

Objective: To establish the determination method for complanatoside A in seeds of Astragalus complanatus.

Method: An HPLC method has been developed to separate complanatoside A on ZORBAX EXTEND-C18 (4.6 mm x 250 mm, 5 microm) column with acetonitrile-water-phosphoric acid (20:80:0.

[Determination of quercetin-3-O-beta-D-glucopyranosyl-7-O-beta-D-gentiobioside in semen descurainiae by HPLC].

Objective: To establish a method for the determination of quercetin-3-O-beta-D-glucopyranosyl-7-O-beta-D-gentiobioside in Semen Descurainiae.

Method: HPLC was used with self-made quercetin-3-O-beta-D-glucopyranosyl-7-O-beta-D-gentiobioside as reference substances.

Result: The average collection was 99.

[Study of cerebral protective effects of naosaitong in animals].

Objective: To investigate the influence of Naosaitong (NST) on the cerebral blood flow (CBF), the infarct areas and blood rheology in animals.

Method: NST's cerebral protective effects were investigated by using middle cerebral artery occlusion (MCAO), bilateral common carotid artery ligation, and carrogeenin-induced thrombus model rats, being administrated with medicine for seven days.

Results: Three dosage groups of NST increased CBF in anesthetized rabbits, reduced the infarct areas in MCAO rats, decreased the physical sign indexes, and water quantities.

[Identification on genuine and pseudo shayuanzi collected from different place by HPLC fingerprint].

Objective: To establish the identification method for genuine and pseudo Shayuanzi by HPLC fingerprint.

Method: The flavone, a kind of active composition in genuine (Astragalus complanatus) and analysed species was analysed by RP-HPLC.

Result: The fingerprints of genuine Shayuanzi collected from different places showed the similar characteristics, but they were different from those of pseudo species A.

[Identification and evaluation of TCM shayuanzi].

Objective: To understand the varieties of Shayuanzi on sale and establish the method for evaluating its quality.

Method: 22 samples collected from all over the country were identified by TLC and the contents of total flavone in them were determined with UV.

Result: All samples could be classified into three varieties, and they could be identified by TLC.