Immunogenicity and safety of an Escherichia coli-produced human papillomavirus (types 6/11/16/18/31/33/45/52/58) L1 virus-like-particle vaccine: a phase 2 double-blind, randomized, controlled trial.

The Escherichia coli-produced human papillomavirus (HPV) 16/18 bivalent vaccine (Cecolin) has received prequalification by the World Health Organization based on its high efficacy and good safety profile. We aimed to evaluate the immunogenicity and safety of the second-generation nonavalent HPV 6/11/16/18/31/33/45/52/58 vaccine (Cecolin 9) through the randomized, blinded phase 2 clinical trial. Eligible healthy women aged 18-45 years were randomly (1:1) allocated to receive three doses of 1.

Effects of long-term fertilization patterns on bacterial community structure and soil nutrients in dryland of yellow soil.

Understanding the responses of soil bacterial community to long-term fertilization in dryland of yellow soil could provide theoretical basis for establishing scientific fertilization system and cultivating healthy soil. Based on a 25-year long-term fertilization experiment on yellow soil, we collected soil samples from 0-20 cm layer under different fertilization treatments: no fertilization (CK), balanced application of N, P and K fertilizers (NPK), single application of organic fertilizer (M), combined application of constant organic and inorganic fertilizer (MNPK), and 1/2 organic fertilizer instead of 1/2 chemical fertilizer (MNP). Illumina MiSeq high-throughput sequencing technology was used to examine the effects of different fertilization patterns on soil bacterial community structure and soil nutrient content.

Transmissibility of hand, foot, and mouth disease in 97 counties of China.

Hand, foot, and mouth disease (HFMD) is a serious disease burden in the Asia-Pacific region, including China. This study calculated the transmissibility of HFMD at county levels in Jiangsu Province, China, analyzed the differences of transmissibility and explored the possible influencing factors of its transmissibility. We built a mathematical model for seasonal characteristics of HFMD, estimated the effective reproduction number (R), and compared the incidence rate and transmissibility in different counties using non-parametric tests, rapid cluster analysis and rank-sum ratio in 97 counties in Jiangsu Province from 2015 to 2020.

[Responses of soil microbial biomass C and P to different long-term fertilization treatments in the yellow paddy soil].

To explore the rational fertilization mode to improve the availability of soil phosphorus (P), we analyzed the changes and coupling characteristics of soil carbon (C) and P, microbial biomass C (MBC) and P (MBP) under different fertilization modes with a successive 22-year field experiment in yellow paddy soil. The experiment had 10 treatments, including no fertilization (CK), single application of nitrogen (N), combination of phosphorus and potassium (PK), combination of nitrogen and potassium (NK), combination of nitrogen and phosphorus (NP), combination of nitrogen, phosphorus and potassium (NPK), single application of organic fertilizer (M), and three organic-inorganic fertilizer combinations (1/4M+3/4NP, 0.5MNP, MNPK).

[Effect of long-term application of NPK fertilizer on maize yield and yellow soil nutrients sustainability in Guizhou, China].

A long-term fertilization field experiment was conducted to investigate the effect of nitrogen (N), phosphorus (P), and potassium (K) fertilizer on maize relative yield, yield-increasing effect and the changes of nutrients in yellow soil in Guizhou Province. Five fertilizer combinations were evaluated, including balanced fertilization (NPK) and nutrient deficiency treatments (N, NK, NP, and PK). The maize relative yield, contribution efficiency of N, P, K fertilizer application, sustainability index of soil N, P, K nutrients, and other indicators were measured.