[Capture and detection of human cytomegalovirus (HCMV) IgM by a recombinant multi-epitope chimeric antigen].

To establish a sensitive and specific antibody-capture enzyme-linked immunosorbent assay (AC-ELISA) method to detect serum IgM against human cytomegalovirus (HCMV) by expressing a recombinant HCMV multi-epitope cheimeric antigen through genetic engineering. The dominant epitopes of HCMV were analyzed and selected by computer software A recombinant multi-epitope chimeric antigen expression vector including HCMV DNA was constructed, and transformed into E. coli BL21(DE3).