Photoinduced Bartoli Indole Synthesis by the Oxidative Cleavage of Alkenes with Nitro(hetero)arenes.

Given the unique charm of dipole chemistry, intercepting N-O=C dipoles precisely generated by designed processes to develop novel reactivity has become a seminal challenge. The polar fragmentation of 1,3,2-dioxazolidine species generated through the radical addition of excited nitro(hetero)arenes to alkenes represents a significantly underappreciated mechanism for generating N-O=C dipoles. Herein, we present a photoinduced Bartoli indole synthesis by the oxidative cleavage of alkenes with nitro(hetero)arenes.

Hierarchical magneto-colorimetric labels for immediate lateral flow immunoassay of chlorothalonil residues.

Quantitative detection of pesticide residues in food and environmental samples using an improved lateral flow immunoassay (LFIA) is of considerable importance for real-time analysis. This paper proposes a highly sensitive LFIA platform based on a hierarchical magneto-colorimetric compact. This compact serves as both the target magnetic enrichment substrate and a photosensitive label.

Boronic functionalized aptamer macroarrays with dual-recognition and isothermal amplification of lipopolysaccharide detection.

A highly sensitive dual-recognition fluorescence amplification method is presented for lipopolysaccharide (LPS) detection based on boronic functionalized aptamer macroarrays with dual-recognition and isothermal amplification. The surface of the polystyrene microplate was firstly carboxylated, and then, 3-aminophenylboronic acid was conjugated to the carboxyl groups through EDC/NHS reaction, creating boronic acid groups as the capture moiety for LPS. A recognition DNA aptamer labeled with the fluorescent dye 6-FAM, which exhibits specificity towards LPS, was selected as the signal reporting moiety.

3D-Bioprinted Hepar-on-a-Chip Implanted in Graphene-Based Plasmonic Sensors.

Precise three-dimensional (3D) bioprinting designs enable the fabrication of unique structures for 3D-cell culture models. There is still an absence of real-time detection tools to effectively track in situ 3D-cell performance, hindering a comprehensive understanding of disease progression and drug efficacy assessment. While numerous bioinks have been developed, few are equipped with internal sensors capable of accurate detection.

Design of Selective PARP-1 Inhibitors and Antitumor Studies.

Designing selective PARP-1 inhibitors has become a new strategy for anticancer drug development. By sequence comparison of PARP-1 and PARP-2, we identified a possible selective site (S site) consisting of several different amino acid residues of α-5 helix and D-loop. Targeting this S site, 140 compounds were designed, synthesized, and characterized for their anticancer activities and mechanisms.

Small Molecule SHP2 Inhibitor LXQ-217 Affects Lung Cancer Cell Proliferation in Vitro and in Vivo.

Background: SHP2 is highly expressed in a variety of cancer and has emerged as a potential target for cancer therapeutic agents. The identification of uncharged pTyr mimics is an important direction for the development of SHP2 orthosteric inhibitors.

Methods: Surface plasmon resonance analysis and cellular thermal shift assay were employed to verify the direct binding of LXQ-217 to SHP2.

Triangle Cl-Ag -Cl Sites for Superior Photocatalytic Molecular Oxygen Activation and NO Oxidation of BiOCl.

BiOCl photocatalysis shows great promise for molecular oxygen activation and NO oxidation, but its selective transformation of NO to immobilized nitrate without toxic NO emission is still a great challenge, because of uncontrollable reaction intermediates and pathways. In this study, we demonstrate that the introduction of triangle Cl-Ag -Cl sites on a Cl-terminated, (001) facet-exposed BiOCl can selectively promote one-electron activation of reactant molecular oxygen to intermediate superoxide radicals (⋅O ), and also shift the adsorption configuration of product NO from the weak monodentate binding mode to a strong bidentate mode to avoid unfavorable photolysis. By simultaneously tuning intermediates and products, the Cl-Ag -Cl-landen BiOCl achieved >90 % NO conversion to favorable NO of high selectivity (>97 %) in 10 min under visible light, with the undesired NO concentration below 20 ppb.

Identification of functional genes regulating gastric cancer progression using integrated bioinformatics analysis.

Background: Gastric cancer (GC) is one of the most common cancers and has a poor prognosis. Treatment of GC has remained unchanged over the past few years.

Aim: To investigate the potential therapeutic targets and related regulatory biomarkers of GC.

Locally Asymmetric BiOBr for Efficient Exciton Dissociation and Selective O Activation toward Oxidative Coupling of Amines.

Two-dimensional (2D) layered photocatalysts with highly ordered out-of-plane symmetry usually display robust excitonic effects, thus being ineffective in driving catalytic reactions that necessitate unchained charge carriers. Herein, taking 2D BiOBr as a prototype model, we implement a superficial asymmetric [Br-Bi-O-Bi] stacking in the out-of-plane direction by selectively stripping off the top-layer Br of BiOBr. This local asymmetry disrupts the diagnostic confinement configuration of BiOBr to urge energetic exciton dissociation into charge carriers and further contributes to the emergence of a surface dipole field that powers the subsequent separation of transient electron-hole pairs.

Combinations of Digital Breast Tomosynthesis and Full-field Digital Mammography for Different Density Types of Breasts.

Background: The combination of FFDM and DBT can significantly improve the diagnostic efficiency of breast cancer, but with the increase of breast radiation absorbed dose.

Objectives: To compare and analyze the radiation dose and diagnostic performance of different mammography positions combinations of digital breast tomosynthesis (DBT) and full-field digital mammography (FFDM) for different density types of breasts.

Methods: This retrospective study involved 1,195 patients who underwent simultaneous breast DBT and FFDM.

Structural insights into caspase ADPR deacylization catalyzed by a bacterial effector and host calmodulin.

Programmed cell death and caspase proteins play a pivotal role in host innate immune response combating pathogen infections. Blocking cell death is employed by many bacterial pathogens as a universal virulence strategy. CopC family type III effectors, including CopC from an environmental pathogen Chromobacterium violaceum, utilize calmodulin (CaM) as a co-factor to inactivate caspases by arginine ADPR deacylization.

Analysis of Maternal and Infant Outcomes and Related Factors of Vaginal Delivery of Second Pregnancy after Cesarean Section.

Objective: To explore the clinical effect of different delivery methods and the safety of vaginal delivery of second pregnancy after cesarean section and analyze the related factors.

Methods: A total of 738 eligible pregnant women who underwent cesarean section from September 2018 to August 2020 were randomly selected from our hospital. Among them, 527 pregnant women successfully delivered vaginally were selected as the observation group, and 211 pregnant women who failed vaginal delivery were selected as the control group.

Clinical Application of Peritoneal Dialysis Catheterization without Capsular Puncture Technique.

Objective: To summarize the advantages of peritoneal dialysis (PD) catheters without capsular puncture (only one pneumoperitoneum needle) puncture technique conducted by our center.

Methods: The study examines the clinical data of PD patients (including the general situation of patients, intraoperative and postoperative characteristics, and complications) undergoing pneumoperitoneum needle catheterization from January 2019 to May 2021 in the Department of Nephrology at the First Affiliated Hospital of Hebei North University (the largest peritoneal dialysis center in Zhangjiakou).

Results: A total of 153 surgical cases were collected.

A Short-Term Hybrid TCN-GRU Prediction Model of Bike-Sharing Demand Based on Travel Characteristics Mining.

This paper proposes an accurate short-term prediction model of bike-sharing demand with the hybrid TCN-GRU method. The emergence of shared bicycles has provided people with a low-carbon, green and healthy way of transportation. However, the explosive growth and free-form development of bike-sharing has also brought about a series of problems in the area of urban governance, creating a new opportunity and challenge in the use of a large amount of historical data for regional bike-sharing traffic flow predictions.

Modeling eye movement in dynamic interactive tasks for maximizing situation awareness based on Markov decision process.

For complex dynamic interactive tasks (such as aviating), operators need to continuously extract information from areas of interest (AOIs) through eye movement to maintain high level of situation awareness (SA), as failures of SA may cause task performance degradation, even system accident. Most of the current eye movement models focus on either static tasks (such as image viewing) or simple dynamic tasks (such as video watching), without considering SA. In this study, an eye movement model with the goal of maximizing SA is proposed based on Markov decision process (MDP), which is designed to describe the dynamic eye movement of experienced operators in dynamic interactive tasks.

Community centered public safety resilience under public emergencies: A case study of COVID-19.

During public emergencies, the level of public safety will be resilient and follow a process from decline to rise. Regarding the concept and influencing factors of public safety resilience, a three-level public safety resilience framework that includes personal, community, and government levels was proposed in this study. It provided the overall metrics that used the resistance and recovery ability to describe the dynamic characteristics of public safety resilience as well as the resilience assessment indexes on three levels.

Pathogen hijacks programmed cell death signaling by arginine ADPR-deacylization of caspases.

Caspases are evolutionarily conserved cysteine proteases that are essential for regulating cell death and are involved in multiple development and disease processes, including immunity. Here, we show that the bacterial type III secretion system (T3SS) effector CopC (Chromobacterium outer protein C) from the environmental pathogen Chromobacterium violaceum attacks caspase-3/-7/-8/-9 by ADPR-deacylization to dysregulate programmed cell death, including apoptosis, necroptosis, and pyroptosis. This modification involves ADP-ribosylation- and deamination-mediated cyclization on Arg207 of caspase-3 by a mechanism that requires the eukaryote-specific protein calmodulin (CaM), leading to inhibition of caspase activity.

Arginine GlcNAcylation and Activity Regulation of PhoP by a Type III Secretion System Effector in .

type III secretion system (T3SS) effector SseK3 is a glycosyltransferase delivered directly into the host cells to modify host protein substrates, thus manipulating host cellular signal transduction. Here, we identify and characterize the Arg-GlcNAcylation activity of SseK3 inside bacterial cells. Combining Arg-GlcNAc protein immunoprecipitation and mass spectrometry, we found that 60 bacterial proteins were GlcNAcylated during infection, especially the two-component signal transduction system regulatory protein PhoP.

Evaluation of Small Molecule Combinations against Respiratory Syncytial Virus In Vitro.

Respiratory syncytial virus (RSV) is a major pathogen that causes severe lower respiratory tract infection in infants, the elderly and the immunocompromised worldwide. At present no approved specific drugs or vaccines are available to treat this pathogen. Recently, several promising candidates targeting RSV entry and multiplication steps are under investigation.

Auditory versus visual spatial stimulus-response mappings in tracking and discrete dual task performance: implications for human-machine interface design.

A discrete four-choice response task with auditory signal presentation and a joystick-controlled visual tracking task was used to investigate how spatial compatibility influences the dual-task performance of different display-control settings. It was found that the more incompatible the stimulus-response mapping, the longer the delay for both tasks, presumably because of the longer stimulus encoding time required for the incompatible conditions. A comparison of the findings of this study with those of past experiments on visual visual setting shows that the dual-task performance in a cross-modality (auditory visual) setting was significantly better than that in an intra-modality (visual visual) setting because of visual scanning required in the intra-modal dual tasks.

Arg-GlcNAcylation on TRADD by NleB and SseK1 Is Crucial for Bacterial Pathogenesis.

Death receptor signaling is critical for cell death, inflammation, and immune homeostasis. Hijacking death receptors and their corresponding adaptors through type III secretion system (T3SS) effectors has been evolved to be a bacterial evasion strategy. NleB from enteropathogenic (EPEC) and SseK1/2/3 from serovar Typhimurium ( Typhimurium) can modify some death domain (DD) proteins through arginine-GlcNAcylation.

Arginine GlcNAcylation of Rab small GTPases by the pathogen Salmonella Typhimurium.

Salmonella enterica serovar Typhimurium, an intracellular Gram-negative bacterial pathogen, employs two type III secretion systems to deliver virulence effector proteins to host cells. One such effector, SseK3, is a Golgi-targeting arginine GlcNAc transferase. Here, we show that SseK3 colocalizes with cis-Golgi via lipid binding.