A new device termed "Optomagnetic Micromirror Arrays" (OMA) is demonstrated capable of mapping the stiffness distribution of biomimetic materials across a 5.1 mm × 7.2 mm field of view with cellular resolution.
View Article and Find Full Text PDFMapping cellular activities over large areas is crucial for understanding the collective behaviors of multicellular systems. Biomechanical properties, such as cellular traction force, serve as critical regulators of physiological states and molecular configurations. However, existing technologies for mapping large-area biomechanical dynamics are limited by the small field of view and scanning nature.
View Article and Find Full Text PDFWe report a massive field-of-view and high-speed videography platform for measuring the sub-cellular traction forces of more than 10,000 biological cells over 13 mm at 83 frames per second. Our Single-Pixel Optical Tracers (SPOT) tool uses 2-dimensional diffraction gratings embedded into a soft substrate to convert cells' mechanical traction force into optical colors detectable by a video camera. The platform measures the sub-cellular traction forces of diverse cell types, including tightly connected tissue sheets and near isolated cells.
View Article and Find Full Text PDFThe recent emerging alternative to classic numerical Fast Fourier transform (FFT) computation, based on GHz ultrasonic waves generated from and detected by piezoelectric transducers for wavefront computing (WFC), is more efficient and energy-saving. In this paper, we present comprehensive studies on the modeling and simulation methods for ultrasonic WFC computation. We validate the design of the WFC system using ray-tracing, Fresnel diffraction (FD), and the full-wave finite element method (FEM).
View Article and Find Full Text PDFUnlabelled: We report a large field-of-view and high-speed videography platform for measuring the sub-cellular traction forces of more than 10,000 biological cells over 13mm at 83 frames per second. Our Single-Pixel Optical Tracers (SPOT) tool uses 2-dimensional diffraction gratings embedded into a soft substrate to convert cells' mechanical traction stress into optical colors detectable by a video camera. The platform measures the sub-cellular traction forces of diverse cell types, including tightly connected tissue sheets and near isolated cells.
View Article and Find Full Text PDFWe demonstrate a novel platform for mapping the pressure distribution of complex microfluidics networks with high spatial resolution. Our approach utilizes colorimetric interferometers enabled by lossy optical resonant cavities embedded in a silicon substrate. Detection of local pressures in real-time within a fluid network occurs by monitoring a reflected color emanating from each optical cavity.
View Article and Find Full Text PDFScar tissue size following myocardial infarction is an independent predictor of cardiovascular outcomes, yet little is known about factors regulating scar size. We demonstrate that collagen V, a minor constituent of heart scars, regulates the size of heart scars after ischemic injury. Depletion of collagen V led to a paradoxical increase in post-infarction scar size with worsening of heart function.
View Article and Find Full Text PDFIntroduction: Metastasis is a fundamentally physical process in which cells deform through narrow gaps and generate forces to invade surrounding tissues. While it is commonly thought that increased cell deformability is an advantage for invading cells, we previously found that more invasive pancreatic ductal adenocarcinoma (PDAC) cells are stiffer than less invasive PDAC cells. Here we investigate potential mechanisms of the simultaneous increase in PDAC cell stiffness and invasion, focusing on the contributions of myosin II, Arp2/3, and formins.
View Article and Find Full Text PDFA plasmonic micropillar platform with self-organized gold nanospheres is reported for the precision cell traction force measurement across a large field-of-view (FOV). Gold nanospheres were implanted into the tips of polymer micropillars by annealing gold microdisks with nanosecond laser pulses. Each gold nanosphere is physically anchored in the center of a pillar tip and serves as a strong, point-source-like light scattering center for each micropillar.
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