A cascade strategy for vinyl chloride-substituted BODIPYs with tunable photophysical properties.

An efficient and transition-metal-free method for the synthesis of unprecedented vinyl chloride-substituted BODIPYs has been developed through tandem Friedel-Crafts, enolization and chlorination reactions. This transformation offers high regioselectivity and stereoselectivity, enabling the synthesis of a variety of β-vinyl chloride-β'-acyl- and β,β'-divinyl chloride-substituted BODIPYs in a one-pot reaction at room temperature. Further functionalization gave a β,β'-divinyl chloride-substituted BODIPY with triphenyl phosphonium moieties, which showed favorable two-photon mitochondrion-targeting imaging capacity in living cells with intense deep-red fluorescence.

Biomimetic Nanoparticles Inhibit the HIF-1α/iNOS/NLRP3 Pathway to Alleviate Rheumatoid Arthritis.

Rheumatoid arthritis (RA) is a chronic autoimmune disease distinguished by inflammatory synovitis. Chrysin can alleviate the inflammatory response and inhibit the progression of RA. However, unfavorable physicochemical properties and nonselective biodistribution of chrysin make it difficult to achieve good therapeutic efficacy.

Involvement of the AVP/OT system and lateral septum in the modulation of anxiety/depression caused by 14-week and 20-week social isolation.

Introduction: Chronic social isolation (CSI) stress leads to numerous maladaptive changes in physiology and psychology, however, very little is known about the effects of longer time-scale CSI and there are divergent views regarding the underlying mechanisms. This study aimed to elucidate the common neuroendocrine mechanisms underlying the maladaptive changes caused by 14-week (14wk-) and 20-week (20wk-) CSI.

Methods: We investigated the impacts of 14wk- and 20wk- CSI on anxiety/depression-like behaviors in male C57BL/6N mice with classical behavioral tests, and the immunofluorescence method was used for quantification of the arginine vasopressin (AVP)/Oxytocin (OT) positive neurons in the PVN and screening out the differential activation brain regions (DABrs) on exposure of tail suspension.

Near-Infrared Twisted Polycyclic Arene-Fused BisBODIPYs through a Tandem Inter- and Intramolecular Scholl Reaction.

Five novel polyaromatic-ring-fused bisBODIPYs have been synthesized from corresponding readily available α-arylBODIPYs through a FeCl-mediated tandem inter- and intramolecular oxidative aromatic coupling reaction. These resultant bisBODIPYs show twisted planar conformations, strong absorptions (ε up to 1.8 × 10 M cm), and good fluorescence emissions in the near-infrared region (NIR, 660-734 nm) and good intersystem crossing efficiencies (Φ = 18.

Leucine 7 is a key residue for mutant huntingtin-induced mitochondrial pathology and neurotoxicity in Huntington's disease.

Huntington's disease (HD) is a neurodegenerative disorder caused by the abnormal expansion of CAG repeats in exon 1 of the HTT gene. Mutant huntingtin (mHTT) associates with mitochondria, resulting in mitochondrial dysfunction and neuronal cell death. However, the underlying molecular mechanisms remain unknown.

Multidimensional Integrated Architectonics for Hierarchical Hydrogels with Enhanced Thermal Conductivity for Effective Burn Healing.

To be the wound dressings that alleviate progressive thermal injury, hydrogels require to have high thermal conductivity, excellent mechanical properties, and outstanding biocompatibility, which are hard to achieve simultaneously. Inspired by the multiscale structure of spider silk, we developed a hierarchical hydrogel architecture. This architecture includes molecular-scale hydrogen bonds between oxygen plasma-treated graphene fibers (OPGFs) and poly(vinyl alcohol) (PVA), as well as within PVA molecular chains.

Mechanically Resilient and Highly Efficient Flexible Perovskite Solar Cells with Octylammonium Acetate for Surface Adhesion and Stress Relief.

Flexible perovskite solar cells (FPSCs) have advanced significantly because of their excellent power-per-weight performance and affordable manufacturing costs. The unsatisfactory efficiency and mechanical stability of FPSCs are bottleneck challenges that limit their application. Here, we explore the use of octylammonium acetate (OAAc) with a long, intrinsic, flexible molecular chain on perovskite films for surface adhesion and mechanical releasing.

Regulating astrocyte phenotype by Lcn2 inhibition toward ischemic stroke therapy.

Astrocytes can be reacted to "reactive astrocytes" after ischemia-reperfusion injury, in which A1 phenotype causes neuronal and oligodendrocyte death, whereas the A2 phenotype exerts neuroprotective effects, thus regulating reactive astrocyte to A2 type is a potential target for stroke therapy. Lcn2 level is highly associated with the phenotypic polarization of astrocytes. We found that silencing the Lcn2 gene by adeno-associated virus (AAV)-Lcn2 shRNA adenovirus resulted in a dramatic decrease in A1-type astrocytes and increase in A2 astrocytes in MCAO mice.

Upregulated astrocyte HDAC7 induces Alzheimer-like tau pathologies via deacetylating transcription factor-EB and inhibiting lysosome biogenesis.

Background: Astrocytes, the most abundant glial cell type in the brain, will convert into the reactive state in response to proteotoxic stress such as tau accumulation, a characteristic feature of Alzheimer's disease (AD) and other tauopathies. The formation of reactive astrocytes is partially attributed to the disruption of autophagy lysosomal signaling, and inhibiting of some histone deacetylases (HDACs) has been demonstrated to reduce the molecular and functional characteristics of reactive astrocytes. However, the precise role of autophagy lysosomal signaling in astrocytes that regulates tau pathology remains unclear.

Double-Helix Electrode Ion Funnel: A New Ion Funnel Design with an Extended Mass Range.

The development of an atmospheric pressure interface (API) with a high ion transfer efficiency and wide mass range is advantageous for the performance improvement of mass spectrometry (MS) instruments. In this work, a novel ion guide, namely, the double-helix electrode ion funnel (DHE-IF), has been developed to enhance the ion transmission over a wide mass range in the rough vacuum region. The DHE-IF consists of two funnel-shaped helix electrodes.

A PDMS/chitosan/MPMs composite film based on multi-field coupling enhancement for African swine fever virus P72 protein detection.

African swine fever (ASF) is an acute hemorrhagic disease in pigs caused by the African swine fever virus (ASFV), which has a high mortality rate and brought great damage to global pig farming industry. At present, there is no effective treatment or vaccine to combat ASFV infection, so early detection of ASFV has become particularly important. Therefore, the PDMS/chitosan/MPMs composite film was proposed to detect ASFV P72.

Effect of Ar on Temperature and Flow Distribution in Monocrystalline Graphene Growth: Inert Gas Is Active.

Monocrystalline graphene growth has always been an intriguing research focus. Argon (Ar) is merely viewed as a carrier gas due to its inert chemical properties throughout the whole growth procedure by the chemical vapor deposition method. In this work, the influence of Ar on temperature and flow fields was investigated in consideration of its physical parameter difference among all involved gases.

Human ANP32A/B are SUMOylated and utilized by avian influenza virus NS2 protein to overcome species-specific restriction.

Human ANP32A/B (huANP32A/B) poorly support the polymerase activity of avian influenza viruses (AIVs), thereby limiting interspecies transmission of AIVs from birds to humans. The SUMO-interacting motif (SIM) within NS2 promotes the adaptation of AIV polymerase to huANP32A/B via a yet undisclosed mechanism. Here we show that huANP32A/B are SUMOylated by the E3 SUMO ligase PIAS2α, and deSUMOylated by SENP1.

Adaptive bipartite time-varying formation tracking control for heterogeneous multi-agent systems with DoS attacks.

Denial-of-service (DoS) attacks and antagonistic interactions may exist in complex networks, which will destroy cooperative communication between agents and thus cannot realize collaborative tasks. Therefore, this paper studies time-varying formation tracking (TVFT) of heterogeneous multi-agent systems (HMASs) with DoS attacks and cooperative-antagonistic interactions. It aims to ensure system communication connectivity and allow followers to achieve distributed secure bipartite TVFT.

Twisted Dipole Ion Guide (TDIG) for Flexible Ion Transfer in Atmospheric Pressure Ionization Mass Spectrometry.

In ambient mass spectrometry, the performance in direct in situ analysis applications has been hindered by the lack of efficient ion-transferring technique between the atmosphere pressure ionization source and the mass analyzer. Building upon the hybrid concept of a stack ring ion guide and multipole ion guide, this study proposes the concept of a reconfigurable twisted dipole ion guide (TDIG) that enables flexible ion transfer between atmosphere and vacuum. Initially, theoretical and numerical studies were conducted to understand the basic ion confining principle of the twisted dipole ion guide, revealing its unique merits in long-distance flexible ion transmission.

O-GlcNAcylation stabilized WTAP promotes GBM malignant progression in an N6-methyladenosine-dependent manner.

Background: Interactions between mesenchymal glioblastoma stem cells (MES GSCs) and myeloid-derived macrophages (MDMs) shape the tumor-immunosuppressive microenvironment (TIME), promoting the progression of glioblastoma (GBM). N6-methyladenosine (m6A) plays important roles in the tumor progression. However, the mechanism of m6A in shaping the TIME of GBM remains elusive.

CCN1 Promotes Mesenchymal Phenotype Transition Through Activating NF-κB Signaling Pathway Regulated by S100A8 in Glioma Stem Cells.

Background: The presence of glioma stem cells (GSCs) and the occurrence of mesenchymal phenotype transition contribute to the miserable prognosis of glioblastoma (GBM). Cellular communication network factor 1 (CCN1) is upregulated within various malignancies and associated with cancer development and progression, while the implications of CCN1 in the phenotype transition and tumorigenicity of GSCs remain unclear.

Methods: Data for bioinformatic analysis were obtained from The Cancer Genome Atlas (TCGA) and Chinese Glioma Genome Atlas (CGGA) databases.

Breaking the Thermodynamic Equilibrium for Monocrystalline Graphene Fabrication by Ambient Pressure Regulation.

Developing high-quality monocrystalline graphene has been an area of compelling research focus in the field of two-dimensional materials. Overcoming growth cessation presents a significant challenge in advancing the production of monocrystalline graphene. Herein, methods for sustaining a steady and consistent growth driving force are investigated based on the single-crystal growth theory.

Identification and Characterization of Linear Epitopes of Monoclonal Antibodies Against African Horse Sickness Virus Serotype 1 VP2 Protein.

African horse sickness (AHS) is an acute, fatal, contagious disease of animals of the family Equidae and is caused by infection with the African horse sickness virus (AHSV). Based on the outer capsid protein VP2, AHSV is classified into nine serotypes (AHSV-1 to -9) with little or no serological cross-reactivity between them. In 2020, AHS outbreaks caused by AHSV-1 were reported in Thailand and Malaysia, marking the first occurrences of AHS in Southeast Asia.

A benzofuran-[]-fused BODIPY trimer enabled by dual TBET and PET mechanisms for high-performance two-photon fluorescence imaging.

A benzofuran-[]-fused BODIPY trimer has been efficiently synthesized, featuring a unique structural design that harmoniously integrates TBET (through bond energy transfer) and PET (photo-induced electron transfer) mechanisms. This trimer boasts exceptional optical properties, including a large pseudo-Stokes shift of 100 nm, an impressive fluorescence quantum yield ( = 0.931), an outstandingly high extinction coefficient (182 100 M cm), a remarkable FEF (fluorescence enhancement factors, 22.

Intra- and inter-network connectivity abnormalities associated with surgical outcomes in degenerative cervical myelopathy patients: a resting-state fMRI study.

Resting-state functional MRI (fMRI) has revealed functional changes at the cortical level in degenerative cervical myelopathy (DCM) patients. The aim of this study was to systematically integrate static and dynamic functional connectivity (FC) to unveil abnormalities of functional networks of DCM patients and to analyze the prognostic value of these abnormalities for patients using resting-state fMRI. In this study, we collected clinical data and fMRI data from 44 DCM patients and 39 healthy controls (HC).

Can urban digital intelligence transformation promote corporate green innovation? Evidence from China.

Can urban digital intelligence transformation (DIT) facilitate corporate green innovation (CGI)? This research uses the staggered difference-in-differences (DID) approach to study how urban DIT, represented by the Artificial Intelligence Innovation Development Pilot Zones (AIIDPZs), affects CGI. Regression results indicate that urban DIT promotes both the quantity and quality of CGI. Mechanisms analysis reveals that urban DIT promotes CGI by facilitating digital transformation, increasing firms' investment in R&D, and facilitating information sharing.